what factors might the amount of bacterial growth in a tube of nutrient broth? tell me faster if we can pls .?
822535
temperature, pH, concentration of nutrient, type of nutrient, type of bacteria, amount of light
258097
Is a simple culture enough?\nthe anerobic won't grow in air the aerobic will grow and give off O2. Yeasts will give off CO2.\nYou may need an autoclave to maintain temperature.\n{sorry not to be more helpful}
687306
Check the Michaelis-Menten equation.It's too long to write it all down for you ;)
263670
Hmmm... judging by your interest in cleaning products and dishwashing detergents, I'm guessing you're in that industry or just a student looking for some answers for a project.\n\n-Insufficient data - \nAre you referring to dishwater in a more sterile kitchen or a dirty restaurant?  Is the piping clean or are we talking rusty piping?  Filtered water or non filtered water? Hmmm... so, at STP you want to figure out this. \n\nHere's what you would need.  First figure out the type of microbes that would typically exist in your sink.  Or if you don't have one, get some kind of bacteria.  Take the whole thing, perform your experiment.  Take some water samples.  Finally, send the water samples to a lab immediately for a count.  You could get a concentration of microbes per given volume.  My advice -- it's all I can do.
713335
There are many factors. I will list a few for starters.\n1) an energy source and suitable nutrients\n2) a waste removal system\n3) suitable pH\n4) suitable osmolarity\n5) suitable oxygen levels (ie aerobic or anaerobic)\n6) no toxic available to kill the cell\n7) suitable temperature\n8) suitable pressure\n9) suitable lighting (sometimes important)\n\nBut most importantly, a suitable medium.
445032
Click on "Add details," post the problem, and, as SMeghead said, someone might be able to help.\n\n(EDIT: You didn't provide too much information. From what you gave, about all we can say is that 12.5% of the solute - 1% from 8% - evaporated. If you need to know how many grams, milliliters, cubic centimeters, etc. evaporated, we'll need more information.)
917922
Well, if you want to measure their growth in milk you need:\n\nPure culture of the bacteria in question, sterile milk, sterile glass tubes and a spectrophotometer.  This will measure the density of the bacteria in liquid culture.  I don't know what equipment you have access too (pipetts, tubes, a spectrophotometer etc).\n\nIf you've isolated the bacteria from milk and want to just measure their growth in "whatever", you can use any sterile growth medium that supports the growth of the isolated bacteria.  Again, measuring growth is most easily accomplished by use of a spectrophotometer.  \n\nGrowing bacteria on a plate to decide which grows faster is fairly insane, unless they have significant differences in growth.  I mean, are you going to stare at an agar plate for 24 hours just to see that at 14 hours 5 minutes a tiny pinpoint on plate A was visible, but it took 15 hours and 22 minutes for a colony of the same size on plate B?  No, of course you're not, it's not even plausible.\n\nWith liquid culture, you can take readings every hour for 8 hours and at 24 hours or something.\n\nYou can also dilute and spread-plate the bacteria from liquid cultures onto agar plates if you have your heart set on using them and achieving an exact count for bacterial populations.  But use of a spec still allows for comparisons between species, even without an exact population number.
57968
If you are trained in microbiology you can tell by eye.  Plate out the bacteria (for single colonies) onto a universal media that is suitable for your target bacteria,  ie CLED, Maconkeys, Blood, Chocolate.  Incubate until there is a growth (usually 24hrs at 37 degreees celcsius is aerobic).  Look at the plate, are the colonies uniform in appearence.\n\nIf you are using liquid culture youu could use microscopy before plating out.  Either a gram stain or a direct (wet prep). This would let you know if you had all gram positives or all gram negatives or all rods or all  cocci or a mixture.  You would still have to plate out to make sure only one colony type grows.
1013746
So you'll need the right Bt strain - Bta or Btk; and not all commercial formulations of Bt contain living cells, so you’ll need some active colonies. I would guess that the nutrient broth that manufacturers use is proprietary. The trouble with entompathogens is that they lose virulence when grown outside of an insect. Manufacturers compensate by developing growth mediums that include insect parts or a blend of certain proteins, antibiotics, nutrients, etc. You can probably find some recipes in research journals but you’ll need to visit a university library or pay for a custom search. You can try Ward's Scientific for culturing supplies http://wardsci.com/  You may also need stirrers or shakers and a place suitable for sterile culture. If your broth gets contaminated with fungi or other bacteria you might just kill all of your bees.\n\nAfter you grow your bacteria, you’ll have to induce them into sporulating. The bacteria themselves do not affect insects; it’s the chemicals used in sporulation. After sporulation, you’re left with a bacterial spore, the husk of an empty bacterial cell and a crystal of toxin (it’s an insect stomach poison with no affect on humans). If done in the proper setting, you can harvest these crystalliferous remains, weigh them to calculate the proper dose and formulate it for easy application. It’s not a simple procedure.
516678
It depends on the food and how long it's left out. It will not rotten in the next 5 minutes, it may in a few hours. If the food has spices or salt it may last longer b/c it will be able to fight off the bacteria.  THis is way too general of a question.
687665
If it hits the floor its got crap on it. the longer there the more crap. I will occasionally use the 5 second rule, but still do a visual, since I'm not very fond of hair, feces, or other "dirts". There are a lot of stuff that you can't see. If you're not going to eat it right away throw it away. Bacteria grows pretty fast at room temperature.
415279
Nutrient broth ingredients:\n\nMeat extract 10.0 gm\nPeptone 10.0 gm\nSodium chloride 5.0 gm\nDistilled water 1000 ml\nMix the ingredients and dissolve them by heating in a steamer. When cool, adjust the pH to 7.5-7.6.\n\nTo make it agar add:\nTo the ingredients as in nutrient broth, add 15 gm agar per litre. Dissolve the agar in nutrient broth and sterilize by autoclaving at 121oC for 15 minutes. Prepare plates and slopes as required.
533720
See good ideas in\nhttp://www.uen.org/utahlink/lp_res/nutri375.html\nhttp://chsweb.lr.k12.nj.us/kshimp/cooking/chemwebquest.htm
346500
PBS usually comes sterile and it has a salt balance that can prevent any hyoer/hypotonic situations, but it is not always used to make growth media. Actually, I have used PBS in molecular techniques, but never in making media.
1114615
Well, apparently, they grow quite well on certain caterpillars ...\n\n:-P\n\n... but, seriously, AFAIK the bacterium is not too fussy, or (as we microbiologists generally call it) "fastidious" about its growth requirements.  I assume it will grow on any "minimal" medium, that is, maybe a bit of Yeast Exatract, + / or tryptone or meat extract, with or without addition of agar.  You did not say whether you wanted a liquid or solid growth medium.\n\nWe find Bacillus species as reasonably - common contaminants from the environment.  Our media are designed to isolate bacteria important to human health, so they are more nutritious than Bacillus thuringiensis would need (AFAIK).\n\nIf you do grow it, be careful with it; many members of this genus produce toxins.  I suspect that Bt toxin might not be very good for humans, too.  IMO, it is probably unwise to try to grow bacteria, unless you have appropriate training and laboratory equipment.  For example, you need to sterilise media and equipment, and properly dispose of wastes, in a safe fashion, and according to local bylaws etc.\n\nI am a microbiologist.
816577
I generally follow the recommendations provided by our safety officer at my academic lab.\n\nIf your liquid cultures can afford the extra volume, you can just add a lot of deionized h2o, that should stop the growing (by lysing the cells).  Then toss in the bio-hazard bag, for the autoclave.  \n\nOr, if you have access to an autoclave, then autoclave the culture, and you can probably dump down the drain.\n\nCheck with your safety officer!!\n\nIt was fun thinking about your question.  Pass on the good Karma!
178427
There is one general fact that all food cause "at some point" bacteria gathering on your food, if they don't have sugar, they might have fibers which still helps building cavaties..\nIf u'd just brushed and had that cucumber, gargle with water and salt or Listerin, after all the tooth paste protection was designed to last for some time in your mouth... and most importantly drink water.