What is a sterile method to transfer bacteria?
827835
Do it in a laminar flow hood. Follow aseptic techniques.\nDepending on your use, you may suspend your bacterial culture in an appropriate medium.\n\nIf you dont have a laminar flow hood, do it the traditional way...\nwash your hands, then put alcohol.\nwork on a clean table - clean it with alcohol.\nlight an alcohol lamp in the area to clean air.\nopen your culture tube near the light.\ntransfer aseptically the bacterial culture to cuvette\n  - do it near light to ensure no contaminants from air\ncover the cuvetter near the light.
916415
Firstly, is the culture tube to be a nutrient broth or a nutrient agar?\n\nWhichever it is, both will have to be autoclaved to remove all other organisms. After autoclaving, don't open until you want to insert the sample. Make sure you have a naked flame in front of you. The area just around the flame is your sterile area. \n\nFor a nutrient agar, heat your innoculating loop in the flame until it is red-hot then remove from the flame and flick it to cool it. Test on one side of the agar to make sure it is not too hot. If too hot, the agar will hiss. Collect your sample of E. coli with the innoculating loop and use your other hand to hold the culture tube. Open the culture tube with as few fingers as possible. Streak the E. coli on the nutrient agar making sure to spread it evenly. Always keep the mouth of the tube near the flame. The innoculating loop should also always be near the flame until the process is complete. Close the culture tube and heat the innoculating loop again.\n\nIf it is nutrient broth, then follow the same steps as above except that you insert the innoculating loop into the broth and shake it. Then close as above.\n\n*Remember... when opening the tubes, always keep the mouth of the tube close to the flame and always sterilise the innoculating loops.*\n\nEDIT: Answer to the replied question.\n\nYeah a micropipette would be easier. You'll need to autoclave the micropipette tips before using and like I said, all the procedures have to be carried out right beside the flame. The mouth of the culture tube has to be in or right beside the flame so that no contamination happens.\n\nIn any case, the innoculating loop or the pipette can be used because the nutrient broth is there for the bacteria to develop colonies and grow so the end result will still be the same.
965939
If you apply a hot wash cloth to them first, then gently push out the pus, but not the blood. Yea, that should help. You can also lance them with a pin that you first sterilize by holding a match to  the pin until it turns red hot.
458497
unless you have an infection, piss is actually sterile...
303081
Purify it. And then have it in a closed system where nothing can get to it
741317
This link is one of the better microbiology websites that I've used.\n\nhttp://www.textbookofbacteriology.net/
702124
You can find practical information from Qiagen website if you use their kits which are quite popular these days.  \n\nIt depends on which methods you use for isolation.  The alkali lysis (see link below) was commonly used to rupture bacteria.  Plasmid DNA (smaller in size and more soluble) is separated from baterial genomic DNA (larger and less soluble).  The plasmid DNA is then separate from proteins by phenol extraction where the proteins are removed to the phenol phase while plasmid DNA stays in the aquous phase.  The plasmid DNA is then precipated with ethanol to complete the purification.\n\nQiagen's kit and others overcome the use of phenol (which is toxic and smelly) by using a solid phase that bind DNA to seperate the contaminating proteins.  The DNA is later eluted from the solid phase to complete the purification.\n\nHere are additional links:\nhttp://biotech.biology.arizona.edu/labs/DNA_isolation_plasmid.html\nThe concept of alkali lysis method of plasmid DNA isolation is shown here:\nhttp://a32.lehman.cuny.edu/molbio_course/plasmid_protocol.htm
279956
Its called barbicide, it is some form of antiseptic/ sterilising solution
1068775
by tranfering them duh
902431
Use RNase that is certified free of any DNase activity. Make sure you use the proper stuff and as a rule for most DNase or RNase use a fresh bottle and keep it to yourself. That way you avoid some of the contamination issues.
483055
Wear your PPE (Personal protective equipment). The safest way would probably be to dispense it in a fume hood, with secondary containment (e.g. inside a container with boundaries). You'll want the secondary containment to contain something that can neutralize the base, such as a weak acid. This is for the liquid.\n\nThe solid sample should be dispensed in a fume hood, using a scoopula to transport the sample after it has been poured out.
171384
bleach\namonia(dont mix with bleach)\nAlcohol(pocibly boose and beer)\nAnti bacterial soap\nSpit/dog slober(ensymes)\nurin(amonia(eewww nasty))\nhot water\nsomething involving a microvave toster or some other heating apliance\nsalt water\nor you could shoot them(aim carefully, bacteria is small)
187254
yes, the bacteria can travel to your fallopian tubes and you can get PID? it's also caused by STD's
265719
kill bacteria and viruses in the bloodstream
265671
Depends on what kind of cloning you are talking about.\n\nThe term "cloning" can be used to describe inserting a foreign gene into a bacteria... or creating a genetically identical copy of organism.\n\nTo create an identical copy... you would require an oocyte (an egg) and a somatic cell.  You'd need to remove all the DNA from the egg and the somatic cell... then inject the DNA into the egg.  Along with other factors and after growing... you will have an organism gentically identical to the orriginal.\n\nCloning genes into bacteria involves a plasmid (circular piece of non-chromosomal DNA).  You would need to open up the plasmid using restriction enzymes (DNA scissors basically) and cut the gene of interest out with the same enzyme.  Mix them then insert into your bacteria by your favorite method (electroporation, chemical method, heat/cold shock, micro-injection).  Let the bacteria grow (all bacteria in a colony are gentically identical to the parent... for the most part)
392177
some bacteriophages were radiolabelled with radioactive phosphorous and sulhur. the sulphur bound to the external  protein coat hile the phosphorous bound to the nucleic acid inside. now, these pahges were allowed to infect a bacterial colony. the infected bacterial colony was then separated and subject to centifugation. it revelaed that while the radioactive sulphur was leftbehind in the phages, the bacterium had contracted the radioactive phosphorous. it was suggested that this was because once the phage infected the bacterium, they transfered their DNA into the bacterium. this is called transformation. this has now been verified by other methods. transformation is a very important method for genetic mutation studies.
410456
I can give you the reference for the procedure that is easy and very effective. Use this article to get an idea:\nhttp://www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=Retrieve&db=pubmed&dopt=Abstract&list_uids=10829079&query_hl=1&itool=pubmed_docsum