what is the standard microbiology test methond in china?
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test method for what?
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International work is normally related to WHO standards.\n\nFor testing you need to go to a library that has a copy of what is called "Standard Methods"\n\nThe EPA version can be found on their publication site.\n\nScroll down to 600479020 Methods for Chemical Analysis of Water and Wastes
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Laboratory certification of what?
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Genus: Shigella\n\nGram negative rod\nEnterobacteriacae\nNon-motile\nFacultative anerobes\nNon-lactose fermenting\nColourless colonies on EMB or MacConkeys agar\nIdentified bye biochemical reaction of by serology wiht\nanti-O antibody in agglutinination test
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The greater the magnification, the more morphology you'll be able to see. 100x is preferable because bacteria are so small. Special stains help to see individual organelles inside bacteria, such as mitochondria, nucleus, cell membrane. Different bacteria take up different stains, which helps to identify them, such as gram positive and gram negative bacteria. The tubercle bacillus that causes tuberculosis will stain red on a blue background with the correct stain procedure.
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Checked my bacteria program on my PDA and all that came up where....neisseria gonorrhoeae, neisseria meningitidis, moraxella catarrhalis and veillonella sp. \n\nFirst off, all I recall about those organisms is that they are aerobic cocci that are gram-negative. The exception being viellonella sp. as it is an anerobe. \n\nSecondly, I only looked for those traits I just mentioned, no idea if any of these meet your other criteria. \n\nThirdly, haven't you been doing tests all semester to narrow it down? The pathogens are limited to what you have studied, so it is not as the scope is that large. Try making a list of all of the organisms you have studied, turn it into a graph with all of the traits possible from all of the various tests...start filling in the blanks and you should have no problem figuring out which organism it is. Our specimen was contaminated and I found no problem determining both the assignment and the contaiminant using this method. \n\nPerhaps it is one of the organisms I mentioned above, it's very likely if you have studied any of them in Microbiology. But if not, use the method I described above and it really is just a process of elimination. \n\nGood luck
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try going to www.aol.com and type in organism by biochemical tests that might help you
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http://www.microbeworld.org/\nhttp://www.bacteriamuseum.org/\nhttp://www.microscopy-uk.org.uk/mag/wimsmall/bacdr.html\nhttp://www.ucmp.berkeley.edu/bacteria/bacterialh.html
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Short Answer:\n\n\ngood Microneutralization test takes 3 days & checks for virus\n(microneutralization changes color when virus present)\n(bad microneutralization test doesn't work)\nHFMD is usually due to Coxsackie virus\nNo accurate microneutralization for HFMD, yet\n\n\nHFMD diagnosed clinically by physical exam (correct over 99%)\nbut areas with epidemics don't have enough physicians\nmost accurate lab test for HFMD is cell culture\n(culture takes 1-2 months & HFMD does not last 2 months)\nso lots of research in making faster accurate HFMD tests\n\n\nOnce patient recovers from HFMD they're immune for decades\ninfects kids because they're least immune, most exposed\nspread by infected mucus, spit, feces (infectious weeks)\nkids get dirty when playing & don't clean up much \n\nNEVER give kids with fever aspirin, it can cause Reye syndrome\n(Reye is rare but fatal liver disease)\n\n\n\n\nLonger Explanation:\n\nA good microneutralization test takes about 3 days & checks for virus\n(Hand Foot and Mouth Disease is due to a virus, usually Coxsackie Virus) \n(Note: a bad microneutralization test doesn't work at all)\n\n\nMicroneutralization is a form of Enzyme Linked ImmunoSorbent Assay or ELISA\n(an antibody reacts with an enzyme to change color once it binds its specific antigen)\n(basically the test changes color if what we're looking for is present)\n\n\nTo read more about ELISA\nSee:\nhttp://www.m.ehime-u.ac.jp/~yasuhito/Elisa.html\nhttp://www.medterms.com/script/main/art.asp?articlekey=9099\nhttp://www.medicinenet.com/elisa_tests/article.htm\n\n\n\nHFMD is most often diagnosed clinically (no lab test, doctor can just tell by physical exam and they are right over 99% of the time).  The most reliable lab test for HFMD is cell culture which can take 1-2 months (HFMD usually does not even last 2 months).   A rapid test would be beneficial, especially in areas experiencing epidemic outbreaks, that don't have sufficient numbers of physicians to see all the cases quickly.  This is the reason there is research into using the faster microneutralization test to diagnose HFMD, but right now it is not acurate enough.  \n\nTo read about microneutralization in detecting HFMD\nSee:\nhttp://www.pubmedcentral.gov/articlerender.fcgi?artid=120643\n(see materials & methods section)\nhttp://www.ijppediatricsindia.org/article.asp?issn=0019-5456;year=2005;volume=72;issue=1;spage=17;epage=21;aulast=Sasidharan\n\n\nYou should not give children with a fever aspirin, because it can cause a rare but fatal liver dysfunction caled Reye Syndrome\n\n\nTo read more about Reye's Syndrome\nSee:\nhttp://www.patient.co.uk/showdoc/40001425/\nhttp://www.healthy.net/scr/HealthHint.asp?Id=73\n\n\nTo read more about treating fevers in kids\nSee:\nhttp://www.ds-health.com/fever.htm\n\nHand Foot & Mouth disease affects mainly children under 10, because they are least immune to it and most exposed.  HFMD is spread by infected nose & throat discharges & feces. (these stay infectious for weeks,  even ater the patient that produced them has recovered)   Kids may not wash their hands much and often touch filthy things, including their own spit & mud.  Once someone recovers from HFMD they are usually immune, for several decades.  \n\nTo read more about Hand Foot & Mouth Disease\nSee:\nhttp://www.ibufamily.org/coxsacki.html\nhttp://www.ezyhealth.com/ezyhealth/medreslib/medconds/MedCondsDetail.cfm?islogin=false&ObjID=8B1212B4-674E-11D4-8DE900508B69ABDA\nhttp://www.webmd.com/hw/parenting_news/ty6231.asp?printing=true\nhttp://www.cdc.gov/ncidod/dvrd/revb/enterovirus/hfhf.htm\nhttp://www.healthtouch.com/bin/EContent_HT/cnoteShowLfts.asp?fname=07070&title=HAND%2C+FOOT%2C+AND+MOUTH+DISEASE+IN+CHILDREN+&cid=HTHLTH\nhttp://www.babycenter.com/refcap/toddler/toddlerills/todrash/11433.html
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Do some research on suppliers in China first. Prepare yourself a list of standard questions to ask, FOB pricing, minimum order quantity, etc etc.
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http://www.emedicine.com/\nJournal of Clinical Microbiology http://jcm.asm.org/\nhttp://www.atcc.org/\nhttp://www.cdc.gov/\nhttp://microbewiki.kenyon.edu/index.php/Microbial_Biorealm\nhttp://www.bact.wisc.edu/Microtextbook/index.php\n\ni know there's more ...
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Go to China and you ll find it
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Try these sites:\n\nhttp://mic.sgmjournals.org/\nhttp://mic.sgmjournals.org/contents-by-date.0.shtml\nhttp://microbiol.org/
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Here is info for China:
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This link is one of the better microbiology websites that I've used.\n\nhttp://www.textbookofbacteriology.net/
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is that a standardized test? dont cheat\ncheater\ncheater cheater pumpkin eater
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1.What bacteria are we talking about (E.coli? Which strain?)\n2.There is no such standard solution for any type of bacteria.\n3.Why on earth would you need such a dilution? \n4.What type of facilites do you have access to (You can isolate single cells by FACs provided you have a proper marker and this expensive equipment)