How do you use an inoculating loop?
815083
You need to sterilize the loop before you do the transfer (pass through the flame of a lit Bunsen burner), the you need to let it cool before you do the transfer, or you will fry all the little bacteria that you want to transfer. Then you can actually do the transfer by getting a sample on the loop from the cuvette, and transferring to the cuvette. Keep the cuvette closed until just before you do the transfer, and close it right again after, to avoid airborne contamination. The same goes for the culture tube if it will be used again.
593312
I'm not sure what the question is? Are you looking for someone to write an entire grant proposal and post it here? I'm not sure if such a grant would be funded, because the means of preventing culture contamination are pretty well known: Work in a sterile environment (tissue culture hood). Spray everything with ethanol before you put it in the hood. Use disposable pipets, etc.
214979
You can check out this site, it explains a step by step technique:\n\nhttp://www.pall.com/biosepra/34696_7290.asp
706549
Try to read the common inside the code.(inside the "/*.... */")\n\nThis function do couple of things:\n1. It is to check the input command.\n2. Set the parent thread to wait. \n3. Create a child thread.\n4. Use the child thread to run the command.
1002331
isolate the pathogen...
762657
use the ftp to upload it and use the loop option\n\n:> peace
237595
PLEASE TRY A SAFER METHOD!!
495637
Soak in the ground.\nEvaporate.
72719
This sounds so excessive! What a crazy teacher you must have. :)\n\nAlright - well...\nThe gravel is the easy part. Use the screen as a sift and hopefully the screen will be fine enough that none of the gravel will get through. \n\nNow the plan of action is dump the powder and salt in the water. [Which I guess will be done in Cup #1.] You'll filter out the powder. The salt will dissolve in the water, and you will evaporate the water later. The question is how to do this with what you have.\n\nI'm thinking the craft sticks are to hold up the funnel. Arrange them over the top of the cup [Cup #2] so that they make a triangle and see if it'll hold up the funnel. \n\nPut a filter paper in the funnel. Pour the mixture in and the powder will collect in the funnel. When you're done, take out that first filter paper and set it aside. Then set up your funnel in Cup #3 with the second piece of paper and filter the mixture a second time just to make sure you got all of the powder.\n\nI was trying to think of what the syringe could possibly be for, and the only thing I can think of is to take the salt-water out of Cup #3 and into the evaporation dishes.\n\nNo idea what the magnifying glass is for.\n\nBut the basic idea is that you sift out the gravel, mix the powder&salt in water, fliter out the powder, then dissolve the water.
816577
I generally follow the recommendations provided by our safety officer at my academic lab.\n\nIf your liquid cultures can afford the extra volume, you can just add a lot of deionized h2o, that should stop the growing (by lysing the cells).  Then toss in the bio-hazard bag, for the autoclave.  \n\nOr, if you have access to an autoclave, then autoclave the culture, and you can probably dump down the drain.\n\nCheck with your safety officer!!\n\nIt was fun thinking about your question.  Pass on the good Karma!
741875
500,000 cells to start.\n1:10 dilution will give you 50,000 cells\nif you do the serial dilution 4 times,\nyou will end up 500,000/10,000 = 50 cells left.
1889
I have included several links that show how to do this.  The first one is the most helpful, I think. The others are just to give you a little more background info or other examples.
848385
Use a plunger.  Seriously.  My friend did it out of exasperation, and it actually worked!
339889
You will get a error because you need a loop condition it cant be just 1. for example to print god 5 times make loop condition like for(i=0; i <=5;i++) then it will print "god" five times.
617896
Anybody who has Kids as a Homeopath I will advise them to use ARSENICUM ALBUM 200 three drops of it in a sip of water will stop it from becoming full fledged flu if given as soon as a kid starts sneezing and you know its gonna develop into Flu just three drops will do the trick !\nA STITCH IN TIME SAVES NINE !\n\nTake care and God Bless you !\nFeel Free to ask me Homeopathic remedies whenever you need to.
336637
Use Abreva from the onset.
297783
you start with one, than add zero.  Add one, add zero.  Add one, add zero.  Continue until you get tired.  Than repeat.