how are virus cultivated in a cell culture?
603453
A cell culture is just a vial/petri dish/growth chamber with living cells it it.  You infect the cells with your virus and the virus will multiply and lyse the cells on its own.  Eventually you will have lots of virus and no cells. The amount produced will depend on the number of cells in the medium and the number of copies the virus makes per cell.
915822
Viruses themselves are not alive. Most virologists consider them non-living, as they do not meet all the criteria of the generally accepted definition of life. They are similar to obligate intracellular parasites as they lack the means for self-reproduction outside a host cell, but unlike parasites, viruses are generally not considered to be true living organisms. Among other factors, viruses do not possess a cell membrane or metabolise on their own.\n\ncompter viruses are not alive either.
613810
Antibiotics work on the protein in a cell's membrane, usually denaturing the proteins and causing the cell to rupture.\n\nVirus do not have a cell membrane. They are not actually living, but instead take over the operations within a host cell. In order for an antibiotic to work on a virus, it would have to rupture the cell membrane of the host cell. In that case, the antibiotic would be toxic, and probably more deadly than the viral infection.
888659
the difference is how they make the cells produce the new genetic material for the viruses. RNA viruses does not require the cells' DNA to reproduce.
1027318
Yes, diseases can transfer through those mediums. Actually, the virus or bacteria can stay alive and active even if the fruits or vegies are well-rinsed.
902971
I'm not sure exactly what you are asking, but I'm assuming you want to know how microbiology is similar to stem cell cultivation.\n\nMicrobiologists use media (agar) that has different types of nutrients embedded into it that promote the growth of the organism they want to isolate.  These plates are placed into incubators that also promote the growth of the organism they are trying to isolate.  Sterile technique is used throughout the process to ensure that the media and specimen is not contaminated.  Colonies with desired characteristics are selected and replated on media several times until only the desired organism is isolated.  It is then tested to determine its species and subspecies before resulting the test and making recommendations.\n\nTissue cultures are cells that have also been plated on media that promote the growth of the desired tissue.  In this case, heart valves.  The innoculated media is then placed into incubators.  Desired tissue is tested and placed on new media in order to create a pure tissue source.  Once the desired tissue is grown, it is tested for the presence of the desired antigens, etc.
75558
Plant tissue culture, also called micropropagation, is a practice used to propagate plants under sterile conditions, often to produce clones of a plant. Different techniques in plant tissue culture may offer certain advantages over traditional methods of propagation, including:\n\nThe production of exact copies of plants that produce particularly good flowers, fruits, or have other desirable traits. \nTo quickly produce mature plants. \nThe production of multiples of plants in the absence of seeds or necessary pollinators to produce seeds. \nThe regeneration of whole plants from plant cells that have been genetically modified. \nThe production of plants in sterile containers that allows them to be moved with greatly reduced chances of transmitting diseases, pests, and pathogens. \nThe production of plants from seeds that otherwise have very low chances of germinating and growing, i.e.: orchids and nepenthes. \nPlant tissue culture relies on the fact that all plant cells have the ability to generate a whole plant (totipotency). Single cells (protoplasts), pieces of leaves, or roots can often be used to generate a new plant on culture media given the required nutrients and plant hormones.
425941
No, they use the entire machinery of the host cell to do that.Viruses do not have their own enzymes.
885960
Technically and scientifically it is called as tissue culture which is the development of tissues from a single parental cell under aspartic labotary condition which leads development of a full organisms also.
508821
no one cultures for viral respiratory infections , culturing is usually done for bacteria . viruses can be detected by doing blood test and checking for antibodies ( rarely done and expensive to do ), very little you can do to fight viral respiratory infections except rest , fluids and mild analgesics . antibiotics dont do anything for viral infections
138736
Viruses are infectious agents that require cells for their reproduction.  The replicate by controlling a living cell’s material independently of the cell’s DNA.  This cell is called a Host.  The viral genetic material can be double stranded DNA, single stranded DNA or RNA.  A virus out side of a living cell is called a virion and is metabolically inert.  (It is also covered by a protein or other coating).  They do mutate readily.\nOnce inside a living cell, the virus sheds it’s coating and deactivates much of the genetic material of the cell, only allowing that which it needs or which do not interfere with their replication.  They then force the cell to replicate their genetic material as new virions which will be released when the cell ruptures.\nOpite gave good info on the size of the virus in terms of linear measurements.  Size, in terns of the number of bases runs from as few as 5,000 bases (single strand DNA) for the H-1 parvovirus to about a half a million base pairs in the largest.\nViruses are not considered to be alive.
641618
"Plaque" is the term used to a population of virus-infected cells growing on a cell-culture plate. \nCells multiplicate on a cell-culture plate until they form a monolayer. This can be seen by the naked eye as a uniform translucid layer spanning the whole surface of the plate. \nBut if a bunch of lytic viral particles (viruses causing cell lysis) were spread onto the monolayer, you´ll see a clear halo where a bunch of infected cells are. Virus are passed from one cell to its neighbours as cells become destroyed by the virus itself. So, after a while, there will be a discrete area in the monolayer, where cells were lysed by viruses passing fromo one cell to another. And the area of these "plaques" grow with time (´cause viruses get to reach more and more cells in the periphery of the plaque). If you grow your culture for too long, the complete monolayer will be destroyed, and you won´t be able to distinguish any plaques.
753317
Bored at work:\n\nYou could look for reverse transcriptase activity in infected cells.  This is a sure sign of RNA being turned into DNA.\n\nYou could purify virus from the aftermath of infected cells.  Then set up experiments to determine the nature of what you've isolated and what its properties are.
359498
A virus (not bacteria) is made up primarily of DNA and a protein coat.  They invade the cells of a living organism and destroy the host's DNA, then inject their own DNA into the host cell.  The result is that the infected cell produces new viruses which then exit the host cell and are released to infect others.  This is done because viruses do not contain cells with the orrganells and compounds necessary to reproduce, so they must get cells from the host to do the work for them.  Of course, the host cell is no longer functional as an intact cell once the virus leaves.
364585
D.\n\nIt produces more viral particles up to the point where the cell cannot hold anymore and bursts. The amount of viral particles released is called the "viral burst size" and depends on the virus species. The released particles infect new cells and continue the cycle. This is why virus growth is an exponential curve.
1040546
the virus likes to mutate. so even if they did have one.. it wouldn't work for very long
1003762
Hi.  I am a scientist who works in the pharmaceutical/biotech industry.  In my field, we do a wide variety of things:\n\nIn terms of cell-based drug discovery assays, we can take cells from a person, grow them in petri dishes and test new drugs that we've developed on them to see if the cells live or die in response to the drug. (Of course, this is in very VERY general terms; it's much more complicated than that).  If we want to test a variety of cell types against a variety of chemical compounds, we can use automated technology called High Throughput Screening... In HTS, very sophotisticated robots carry out 96 or 384 experiments at a time.   \n\nMolecular biologists can change cells by adding viruses or DNA plasmids and allowing the virus or plasmid to assimilate itself into the host cells's normal DNA.  This process is called transfection.  This is useful if you want to overexpress a gene or condition that you want to study or model/test a drug against.  Molecular biologists can also clone these plasmids and grow more of them for large-scale experiments.  Two other common techniques that molecular biologists use are PCR (in which we can look at an organism's  genes) and Western Blots (that allows us to look at certain proteins that are produced / expressed within cells).\n\nHistologists can take tissues and use immunohistochemistry (IHC) to detect the presence of certain proteins or other characteristics of the cells.  In IHC, antibodies which targe a certain cellular protein are applied to a preserved tissue and that antibody can then be detected by fluorescence or an enzyme-based coloration process.  \n\nThese are just a few of the techniques that we use to develop new drug therapeutics.  Be aware that the process to develop one drug from start to finish takes about 15-20 years and about $1billion, which might give you an idea of exactly how complicated it can get once you get into detail on the topic.