What is culture media in biology?
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culture media is the media in which necessary elements for the growth of cells or organism. \n\nthese media are designed to give appropriate nurients to the organism for their development. the various parts as carbohydrates(usually a sugar glucose or fructose or complex media), amino acids, various salts etc are present.\n\nthe culture media can be classified in many ways as:\n\n1. complex or defined media: - \ncomlex is the one in which extracts of various organisms are used\ndefined media are those in which each component is added to make the final media.\n\n2. selective media and general media:-\nselective media is one in which we have added somethings which can be used by some perticular organisms only.\nwhereas general media are general for a large population.as the LB(luria bertani) is a general media.\nand many more classifications are there.\n\nso in a way culture media are the way to grow and develop organisms in artificial conditions for being used for studied.
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Try the websites www.protocol-online.com & www.cellculturechat.com\nThe first has protocols for most scientific applications, the second is a forum specifically for cell culture scientists. Good luck!
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multicellular organisms
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Cell lines are produced when you take cells from an organism, propagate it and maintain them in an environment that mimics their natural state. A monolayer is a single layer of cell that cover a culture dish.
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Depends if you want to grow pure cultures of microbes or not. If you don't have to have pure cultures, just put your leftovers in the fridge and forget'em. ;-)\n\nIf you want to grow pure cultures, you will need to know aseptic technique and what each type of microbe likes to eat. \n\nSome will go on a defined medium. That is one that you know exactly what it in there. Some have to have something complicated that we don't know exactly what all the components are, like yeast extract, beef heart infusion, that sort of thing.\n\nThen you need to know the temperature and humidity requirements of your bug and any special atmosphere conditions it need, like low oxygen or no oxygen.\n\nIt also helps to know how to store samples of your bug for future use. Can you freeze it? or do you have to lypholyse it?\n\nHope this helps.
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there are many biological tools. few of them are,\n\nIncubators : which are used for growing microbes on laboratory media...they are maintaiined at different temperatures for bacteria, fungi and algae.\n\nLaminar air flow and saftey cabinets: used for carrying out microbiological work aseptically.\n\nAutoclave : Used for sterilization of microbiological medias\n\nBalances : Used for weighing media's\n\nhope that's enough for you.
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http://www.biologymad.com/master.html?http://www.biologymad.com/a2chqus/a2chqus.htm\n\nhttp://www.salisbury.ac.uk/Login/old_intranet/Learning_Resources/old%20a%20level/PDFS/SCREENS/S30A.PDF#search='A2%20Human%20Biology'
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Procedures used to prevent the introduction of fungi, bacteria, viruses, mycoplasma or other microorganisms into cell, tissue and organ cultures. Although these procedures are used to prevent microbial contamination of cultures, they also prevent cross contamination of cell cultures as well. These procedures may or may not exclude the introduction of infectious molecules.
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sounds like they are all subcultures of cell culture?
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It is the developing of technologies on the molecular scale in the field of biology. That's really the only way to describe it, I think. Why not look it up on wikipedia?
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Microbiology is the study of tiny organisms...be they bacteria, algae, fungi, helminths, etc.\nMicrobio knowledge can be used in biotechnology....for instance, knowledge of DNA and how it works can be applied or made useful to various situations via biotechnology...be they genetic engineering, DNA restriction analysis to determine human relationships, etc.
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YOU WILL LOVE THIS SITE.\nIT IS INTERACTIVE, ANIMATED & SOOO FULL OF KNOWLEDGE.\nTRUST ME!\nhttp://www.mhhe.com/biosci/esp/2002_general/Esp/default.htm
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here u find article for tissue culture
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a positive culture...need antibioltic
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M. tuberculosis is grown on egg based media in special environmental conditions. The culture emdia also contains glycerol and malachite green.
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Plant tissue culture, also called micropropagation, is a practice used to propagate plants under sterile conditions, often to produce clones of a plant. Different techniques in plant tissue culture may offer certain advantages over traditional methods of propagation, including:\n\nThe production of exact copies of plants that produce particularly good flowers, fruits, or have other desirable traits. \nTo quickly produce mature plants. \nThe production of multiples of plants in the absence of seeds or necessary pollinators to produce seeds. \nThe regeneration of whole plants from plant cells that have been genetically modified. \nThe production of plants in sterile containers that allows them to be moved with greatly reduced chances of transmitting diseases, pests, and pathogens. \nThe production of plants from seeds that otherwise have very low chances of germinating and growing, i.e.: orchids and nepenthes. \nPlant tissue culture relies on the fact that all plant cells have the ability to generate a whole plant (totipotency). Single cells (protoplasts), pieces of leaves, or roots can often be used to generate a new plant on culture media given the required nutrients and plant hormones.
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Even single celled organisms engage in an exchange of genetic material, aka s e x.  One organism extends a pili and genetic material is exchanged between it and another microbe that lacks a pili.