what bacteria groes brite yellow in simmons citrate agar?
487689
It might be micrococcus.
310739
Here is a short answer for you.  :)\n\nGram negative bacteria have a lipopolysaccharide (LPS) molecule attached to their outer cell membrane.  LPS is an endotoxin that triggers an immune response in the form of fever and eventually shock as the toxin accumulates in the bloodstream.  \n\nGram positive bacteria do not have LPS.\n\nP.S.  There is no such species as Pseudomonas aureus  :)
469700
there are lots of ways to test water.  plating a water sample on EMB or MacConkeys agar will show if bacteria present are enteric bacteria.  if the sample has very few bacteria present you may have to filter a larger volume through a sterile 0.2 micron filter to collect enough bacteria to examine.
116081
OK.  I've searched all of my micro stuff and I can't find mention of a PCR anywhere.  You'd figure someone who could answer this question would know what a PCR was, but not this someone.\n\nAll I can say is that for years people relied on bacterial morphology (Spherical, Rods, Curved, Clusters, Chains, etc. . .), grams stain characteristics (+ or -), colony morphology, atmospheric growing conditions (O2, no O2, increased CO2), nutritional requirements, and biochemical tests (Peroxidase, Indole, Oxidase) to differentiate different species of bacteria from each other.\n\nI hope this is what you're looking for.
684712
I'm not sure I understand the question, but I'll try anyway.  :)  If you are trying to determine IF you have both Pseudomonas and Klebsiella on in your culture, you can streak a MacConkey agar plate with the culture.  Pseudomonas cannot ferment lactose, so it will grow on the plate but remain colorless.  On the other hand, Klesiella can ferment lactose, so it will also grow on the plate but turn bright pink.  That's probably the easiest way to distinguish the two from a mixed culture.\n\nAs for the "local disinfectant" part of the question...are you trying to pull out these bacteria and then do disinfectant susceptibility testing on them?  If so, you can streak out the plate as mentioned above, then subculture each so you can get a pure culture.  Then, you do disinfectant tests on the bacteria separately by the disk diffusion method, etc.\n\nI hope that helps some!
599698
Try using a pro-biotic product. The bacteria in the product digests waste. They use this in commercial ponds for cleaning before restocking, and also in oil spills, because it can digest petrol. Ask your local house and garden supplies shop, or if you live near farms ask them.
745174
E coli loves a basin or the floor or basically anything that has food for it. I suggest you go to a store that sells scientific equipment and buy a petri dish with agar gel in it. Agar gel is a substance that enables bacteria to grow on it. You should take your sample and gently rub it on the agar gel, but be sure that you do not let the dish be exposed too long to the air, because bacteria is everywhere and you might get some unwanted cultures on your dish.\nYou must then keep the dish at room temperature for at least three days. \nThe E.coli will be visible on the agar gel, but for best viewing of the culture, a microscope is needed, because without it you will only see a yellow line where you rubbed the sample.\nA microscope is a scientists best friend, because with that we can see worlds that other people did not even know existed. you definitely need a microscope.
852371
1) Basic\n2) BTB turns yellow in a base\n3) HCI + LiOH -> H2O + LiCI\n\nI think these should be right.
822279
The bacteria use the starches and carbohydrates in the agar to multiply. The purpose of agar is to be a universal growth medium for all bacterial colonies to grow
204997
FastMedia™ LB is a LB based medium supplemented with selective antibiotic used for propagation of resistant bacteria in shake-flasks.\n\nFastMedia™ X-gal is a LB based agar medium supplemented with 100 µg/ml X-gal, 100 µg/ml IPTG andselective antibiotic used for detection of blue-white resistant colonies.
654500
Many of them oxidize H2S\n\nso its like water but using sulphur, thats why a lot of these bacteria are purple. \n\nheres an abstract on how they work, or at least will give you a general idea ans some examples!\n\nSeven strains of heterotrophic iron-oxidizing acidophilic bacteria were examined to determine their abilities to promote oxidative dissolution of pyrite (FeS2) when they were grown in pure cultures and in mixed cultures with sulfur-oxidizing Thiobacillus spp. Only one of the isolates (strain T-24) oxidized pyrite when it was grown in pyrite-basal salts medium. However, when pyrite-containing cultures were supplemented with 0.02% (wt/vol) yeast extract, most of the isolates oxidized pyrite, and one (strain T-24) promoted rates of mineral dissolution similar to the rates observed with the iron-oxidizing autotroph Thiobacillus ferrooxidans. Pyrite oxidation by another isolate (strain T-21) occurred in cultures containing between 0.005 and 0.05% (wt/vol) yeast extract but was completely inhibited in cultures containing 0.5% yeast extract. Ferrous iron was also needed for mineral dissolution by the iron-oxidizing heterotrophs, indicating that these organisms oxidize pyrite via the "indirect" mechanism. Mixed cultures of three isolates (strains T-21, T-23, and T-24) and the sulfur-oxidizing autotroph Thiobacillus thiooxidans promoted pyrite dissolution; since neither strains T-21 and T-23 nor T. thiooxidans could oxidize this mineral in yeast extract-free media, this was a novel example of bacterial synergism. Mixed cultures of strains T-21 and T-23 and the sulfur-oxidizing mixotroph Thiobacillus acidophilus also oxidized pyrite but to a lesser extent than did mixed cultures containing T. thiooxidans. Pyrite leaching by strain T-23 grown in an organic compound-rich medium and incubated either shaken or unshaken was also assessed. The potential environmental significance of iron-oxidizing heterotrophs in accelerating pyrite oxidation is discussed.
1075584
This is a great source for you:\nhttp://www.blackwellpublishing.com/ogunseitan/student.asp\nEnjoy the microbes!\n;-D
264070
What is meant by "Type" ?\n\nMicrococcus luteus, is a yellow very small circular bacterium.  They are gram-negative I believe. (50/50 shot and I don't have my chart at easy access)
725203
I can't remeber off the top of my head but there is a wonderful book out there called Bergey’s Manual of Determinative Bacteriology 9th Edition that should be able to answer this question for you.
418502
Title: SEAWEEDS FARMING : Bureau of Fisheries and Aquatic Resources (BFAR) Regional Office IX\n\nBrief Description: Manufactured Phytocolloid Carageenan come from the extract of Eucheuma (Goso). The raw material contains high ash that resembles agar requiring only higher concentration to form gel. Apparently used to manufacture surgical jellies, salves and ointment. Also an essential ingredients in pudding, frosting and baker jellies for emuLsification and improvement of its texture. An important stabilizer for ice cream, confectioners, syrups and thickener for soup and related products \n\nHope this helps.
934765
The TSA color change indicates a pH change. \n\nHere is a cut/paste from an online lab manual:\n\nTSIA slant tubes contain lactose and sucrose in 1% concentrations and glucose in a 0.1% concentration (which allows for the detection of the utilization of this substrate only).  It is used to differentiate among the different genera of Enterobacteriaceae, and to distinguish the Enterobacteriaceae from other Gram-negative intestinal bacilli.  This differentiation is made upon the basis of differences in carbohydrate fermentation patterns and H2S production.  The acid-base indicator (Phenol Red) is also incorporated.  A color change from an orange-red color to a yellow color indicates the presence of acids.  An alkaline slant (red) and an acid butt (yellow) indicates that only glucose fermentation occurred.  An acid slant with an acid butt indicates that lactose and/or sucrose fermentation has occurred.  No color change (red) indicates that no carbohydrate fermentation has taken place.  \n\nTSIA also contains sodium thiosulfate.  This substance may be metabolized to produce H2S and ferrous sulfate.  Following incubation, only cultures of organisms capable of producing H2S will show an extensive blackening of the butt due to the precipitation of the insoluble ferrous sulfide.
183362
they have a website...or are you looking to buy one? try trying in the name of the bacterium into a search engine. most college's have at least one.