several culture media are in use in microbiology lab,discus with basis of their continous uage?
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Some media grow most bacteria.  Blood Agar\nSome media usually used for anaerobic bacteria.\nChocolate Agar (not really chocolate, that's just the name)\nSome media of various types are used when the bacteria or fungus will only grow on certain nutrients.\nSearch on Mycobacteria Culture Media\nSearch on Fungus Culture Media\n\nThe most commonly used media other than the above are called Selective Culture Media\nhttp://myweb2.search.yahoo.com/search?fr=ks&p=Selective+culture+media\nThese grow some bacteria while inhibiting others\na typical one is Mannitol Salt Agar for culturing staphylococci\n\nSometimes liquid media are used for studying bacteria, also for saving them in a safe place on the way to the lab.\n\nThere are many different types of the latter.  Also used for studying how fast they grow.\n\nA complete answer is so long evryone would be turned off and no one would read it, probably including you.\n\nSo go to the url above and read, that is a good starting place.\n\nAnaeorbic bacteria are grow in an oxygen poor enclosure placed into the incubator.\n\nBadly run microbiology labs are a great source of potentially infective hospital bacteria.  They need to be well coordinated with the Infection Control personnel, thus aiding this effort.\n\nUsed media and cultures must be totally sterilized, especially where antibiotic sensitivity testing is involved.\n\nThat's the lab part in reducing environmental pollution and controlling the spread of antibiotic-resistant bacteria.
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Yeast can grow on all sorts of agar plates.  They don't need fancy cell culture conditions like mammallian cell lines do.\n\nhttp://www.midsci.com/docs/qbio/ChapterPDF/EU_04.pdf#search='media%20for%20yeast%20growth'  those guys sell some yeast growth media.  \n\nA kind I've used before is SAB (per liter: 40g glucose/10g peptone/5g agar).\n\nI would go with agar plates instead of liquid culture because it will be easier to insure uniform exposure.  The yeast should also remain viable for longer on plates than in liquid culture. \n\nThis sounds like a clever idea for an experiment, good luck.
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Several companies make packs that generate CO2 and N once opened.  They also bind atmospheric oxygen (O2).  If you plate your specimen on an anaerobic media, then place the media in an airtight container with one of these packs, you should acheive the appropriate environment. (You can use an anaerobic indicator to make sure that you've acheived the right air mix.)\n\nWhile plating the organisms, be sure to be quick.  Some organisms are killed by O2, so the longer it takes you to plate them, the more chance there is for O2 to get in and damage them.
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Microbiology is the study of microorganisms, which are unicellular or cell-cluster microscopic organisms. This includes eukaryotes such as fungi and protists, and prokaryotes such as bacteria and certain algaes. Viruses, though not strictly classed as living organisms, are also studied[1].\n\nAlthough much is now known in the field of microbiology, advances are being made regularly. The most common estimates suggest that we have studied only about 1% of all of the microbes in any given environment. Thus, despite the fact that over three hundred years have passed since the discovery of microbes, the field of microbiology is clearly in its infancy relative to other biological disciplines such as zoology, botany and entomology.\n\nYou could get more information from the link below...
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Microbiology is a very broad field. what are you interested in? doing the investigatory would be more fun and learning would be easier if you were into it. anyways, here are some sites. go check them out. \n\nhttp://www.microbes.info/forums/index.php\nhttp://www.suite101.com/welcome.cfm/microbiology\nhttp://www.asm.org/\n\nI had microbiology last semester. we tested the antimicrobial property of some plants. my friends tested the effectivity of some of the facial wash available on the market. some tested the effect of hair gel on microbial growth. you can do lots of things. i read a study where they tested the plastic degrading activity of microbes found in mangroves. still there are those who go to dumpsites and tests the microbes degrading activity. then there are those microbes that are used as air filters. the list goes on and on.
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you can surely rule out some things based on the type of plate you are using.  assuming that you have covered that base, you can separately pick a colony that you think may be a contaminant and look for differences in growth patterns, color, morphology, gram stain, growth on other media, and if all else fails, you can sequence it to find out exactly what it is.
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You have to produce the specimen on your own.  Reading/viewing materials are often available in a private room.
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to avoid  contaminating the sample
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To isolate it, take a sample, dilute it then take a sample from that & spread it on a sterile petri-dish.\n\nYou can then take a sample from a pure culture.
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multicellular organisms
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You can get disposable, sterile swabs.  These would probably work the best.  You should be able to get them via online... google "lab equipment swabs".\n\nopen your swab, being careful to not touch the tip.  Run the swab over the section you want to culture.  Then, simply smear the swab onto the petri dish.  \n\nFor the petri dishes, you probably want more than just an empty dish.  You want an agar plate.  An agar plate is a gelatinous material on the bottom of the dish that provides food and nourishment for the bacteria.\n\nYou could probably use gelatin as a stand-in.  Buy plain gelatin and make a batch twice as concentrated as it should be.  This will make the gelatin harder and less likely to melt at room temperature.\n\nGood luck!
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For someone just finnishing a microbiology course, he/she knows very little about it. Microbiology is the study of microscopic organisms. These include bacteria, fungi, viruses, and parasites such as amoeba and helminths (a type of worm) as well as eukaryotic single celled organisms like yeast.\n\nIt is the greatest subject to studying in biology.\n\nThis website has some good information on it related to microbiology and virology (study of viruses). Im really enjoying my degree, and if you are thinking about taking it, then i recommend you do, and come to Uni of Leicester!!\n\nhttp://www-micro.msb.le.ac.uk/
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There are both culture-dependent and culture-independent ways of doing this...\n\nCulture-dependent: you can centrifuge a soil sample and then plate out the supernantant onto various types of agar.  If you are looking for bacteria, you can use agars that enrich for certain species like Streptomyces or Actinomyces Isolation Agar, or you can use more general agar like Tryptic Soy Agar.  Just be sure to include cyclohexamide in the media to prevent fungi from taking over!  If you are looking for viruses, you will have to do a virus/phage prep from the soil supernant and then plate onto lawns of bacteria or fungi, depending on what types of viruses you are looking for.  If you are looking for fungi, you can use the same supernatant but use media for fungi enrichment, such as Potato Dextrose Agar or Worts Agar.\n\nCulture-independent:  you can isolate DNA from the soil and do sequence analysis to see what kind of bacterial/viral/fungal commuunity there is in the soil.  There are various kits you can buy to do this, dependding on which organism's DNA you want.  This is a much faster method than growing them on agar, but you miss out actually seeing the organisms, as well as enumerating them.
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Procedures used to prevent the introduction of fungi, bacteria, viruses, mycoplasma or other microorganisms into cell, tissue and organ cultures. Although these procedures are used to prevent microbial contamination of cultures, they also prevent cross contamination of cell cultures as well. These procedures may or may not exclude the introduction of infectious molecules.
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there are many biological tools. few of them are,\n\nIncubators : which are used for growing microbes on laboratory media...they are maintaiined at different temperatures for bacteria, fungi and algae.\n\nLaminar air flow and saftey cabinets: used for carrying out microbiological work aseptically.\n\nAutoclave : Used for sterilization of microbiological medias\n\nBalances : Used for weighing media's\n\nhope that's enough for you.
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A cell culture is just a vial/petri dish/growth chamber with living cells it it.  You infect the cells with your virus and the virus will multiply and lyse the cells on its own.  Eventually you will have lots of virus and no cells. The amount produced will depend on the number of cells in the medium and the number of copies the virus makes per cell.
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bacteria, viruses, cyanobacteria or fungi?  or do you mean specific examples