How could you test to see if a bacterium has taken in a human growth hormone plasmid?
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human don't have plasmid. but if you're asking how to test a bacterium for human growth hormone (hgh) recombination into its plasmid, you can perform is PCR test for the HGH mRNA. that should be the most direct proof.\nor you can do a DNA sequencing, but that takes longer.
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http://www-sequence.stanford.edu/group/techdev/plasmid.html
445089
Yes ther is .. just visit this http://labs.google.com/
57968
If you are trained in microbiology you can tell by eye.  Plate out the bacteria (for single colonies) onto a universal media that is suitable for your target bacteria,  ie CLED, Maconkeys, Blood, Chocolate.  Incubate until there is a growth (usually 24hrs at 37 degreees celcsius is aerobic).  Look at the plate, are the colonies uniform in appearence.\n\nIf you are using liquid culture youu could use microscopy before plating out.  Either a gram stain or a direct (wet prep). This would let you know if you had all gram positives or all gram negatives or all rods or all  cocci or a mixture.  You would still have to plate out to make sure only one colony type grows.
394349
This article might help if you reproduce their test system:\nhttp://www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=Retrieve&db=PubMed&list_uids=15159161&dopt=Abstract
490917
The phenotype is the physical expression of the genotype. In bacteria transformed with the pBLU plasmid and plated on the growth media containing IPTG and X-Gal will grow as either white colonies or blue colonies. pBLU is a plasmid that contains part of a gene called beta-galactosidase. The portion of the plasmid that contains this gene is called the multiple cloning site (MCS). (If you want to introduce foreign DNA into the plasmid, this is where you would introduce it.) The plasmid also has a gene for antibiotic resistance. The bacteria you use for transform with this plasmid must not already have this type of antibiotic resistance. If the bacteria are transformed with pBLU and plated on media with anibiotics only the bacteria that recieved the plasmid will grow. Those that were not transformed will die. If no DNA was inserted into the MCS of the plasmid then the for producing beta-galactosidase will be intact. This gene is called Lac Z. The transcription of this gene must be induced with a chemical called IPTG. So if the agar plate contained IPTG then the Lac Z gene will be induced and the protein beta-galactosidase will be produced. If this protein is produced it will cleave the chemical X-Gal (which was also on the plate) causing the X-gal to change from clear to blue.This causes the colonies to have a blue phenotype.\nIf the Lac Z gene was interrupted by foreign DNA in the MCS then no beta-galactosidase will be produced and the colonies will grow white.
262489
There is nothing for it in alternative medicine. You may qualify for human growth hormone in the judgement of your endocrinologist.
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I wouldn't think so since blood agar is already a semi-solid. A coagulase test really should be done to a liquid to see if it turns into a semi-solid.
195100
Apparently he gets tested and has always passed, but there's no test for Human Growth Hormone which he allegedly used.\n\nSee the article below for more information.
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1) Collect your microbial agent\n2) Inoculate it into enriched media\n3) Place 3 mm size ring of ointment onto the agar\n4) Check in 24 hours and measure the inhibition zone
615040
Its a genuine question; I suggest you to post ur question in google answers; u can get researched answers; remeber it is paid service. What I know about is it is produced by recombinant technique which word itself is self explanatory; meaning a complicated genetic process GH like proteins are made with the knowledge of its DNA structure. It has been used successfully in several conditions which can produce short stature. They are genetic short stature, Turners syndrome, Prader willi syndrome, Chronica renal failure. The subjects are shown to be better in height velocity and has achieved a target height height 50 th percentile in a significant number of cases. It is an injection through subcutaneous route; intermittent ie once in few weeks; and requires monitoring of height BP and some blood tests to look for side effects. No significant harm has been reported till now. You can get these details in medical text books like; Harrison's internal medicine; Nelson's text book of paediatrics and various books of endocrinology. For the website you can do a google search with key words Human Growth Hormone, Treatment with growth hormone, adverse effects Human growth hormones etc and select appropriate articles according to ur knowledge level..Look for emedicine article; if u r a medical professional u can look for pubmed article..
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figure out the efgfect of the enzyme. Or you can test DNA structure. Inhibitary enzymes have a blacked actionsite whereas activation enzymes have activation sites open to substrates
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here are lists and pictures\n\nhttp://www.buddycom.com/bacteria/gpc.html
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Plasmids often contain genes for drug resistance and are used in genetic engineering, since they can be transmitted between bacteria of the same and different species.\n\nThat's the only one with any survival qualities.
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your teacher needs to work bettering his/her questions. Or you need to re-phrase the questions so their is more detail.\n\nI was given a similar question on a take home exam, but I think it contained some more information, so I actually learned something.\n\nWith the information given, the one thing you can find out about this plasmid is what gene it posseses that allows its antibiotic resistance.  \n\nYou would do this by plating out the plasmid on agar plates that contain different antibiotics i.e. kanamycin, ampicillin.\n\nYou can pick the colonies that grow, and test for the "glo" gene.  This would verify the presence of the antimicrobial gene from which the colony was picked from.\n\nIn other words, the colony which has the "glo" gene also contains the gene resistant to the antimicrobial agent it was on  grown on.\n\n\nI hope this helps.  Pass on the good Karma!
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I think you might be refering to plasmids.\nA plasmid is a small circular piece of DNA found in bacteria. A plasmid will often include genes which encode a protein that confers resistance to antibiotics. They can be transferred fromn one bacterium to another and replicate separately from the bacterial genomic DNA. One bacterium can contain many copies of a particular plasmid.\n\nLook it up & see if it is relevant to your enquiry.
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The mRNA transcripts from the hypothalamus are processed transcripts with their introns are deleted. So we use reverse transcriptase to convert the hGH mRNA to cDNA that represent the gene of hGH but without introns ( just exons). Because bacterial cells don't have introns we use cDNA to introduce foreign genes into bacterial cells to produce recombinant products.