I need to make a 3-D model of a phospholipid bilayer and also an embedded protein and need help?
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Here is the one I recently made:\n\nTake a transparency sheet and take ear buds (used for over head projectors)- lots of them. Dip the top of cotton part, of a bunch of ear buds, in glue and place them vertically on the transparent sheet. Go on doing this to make a square tightly filled with ear buds.  Note: you will have to give support to the ear buds until they are glued completely. \n\nNow you can apply the glue on the other side of ear buds and place another transparent sheet. Paint the central region of the ear bud-sticks with black colour. If required, cover the sides. It now looks like a square piece taken from a region in the plasma membrane.\n\nThe cotton ends now look like the hydrophobic layers on either end while the central region looks like the hydrophilic ends of phospholipids. If you want to elaborate, you can include proteins in the bilayer! You can use colour papers, ribbons etc (you can stiffen them by dipping in some glue and drying in the shape you want; I have also seen hair-bands available that form specific ion-channel like structures. Goodluck!
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http://www.fhcrc.org/science/shared_resources/proteomics/peptide_synthesis/basics/spps.html\nTry this one. I hope it helps
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You can get online tutorials. A great one is HTML Goodies. It explains in modules each concept with concrete examples.
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You're probably referring to the plasma membrane. The interior is hydrophobic, while the exterior is hydrophillic. The phospholipids can translocate horizontally within the bilayer, but can't cross from one layer to the other. So, the membrane behaves like a fluid.  See MIT's website for more info. the link is below
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Since they are the same size and charge a normal gel would not work, and I'm not sure that a 2D gel would work either. I think isoelectric focusing is supposed to be a very sensitive method for separating similar proteins, so you might look into that.
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check out this tutorial at this really cool site...it's illustrated and it worked for me ....good luck
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Eukaryotic cell--use modeling clay.
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1 ribosomes\n2 stimuli\n3 enzyme\n4 cytoplasm
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I jsut did the same project with my 13 year old son.\nThis is what we used\n\nhttp://www.enchantedlearning.com/subjects/plants/cell/anatomy.GIF\n\nwe had to make a model
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◙ use this tool to make the html\n\n► http://cit.ucsf.edu/embedmedia/step1.php
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http://www.webreference.com/3d/ Try that, good luck with the assignment.
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The Development of a Scientific Fact \n\nThe fluid-mosaic membrane model as a scientific fact \nWhat the model says \n\nThe fluid mosaic model of a biological membrane defines a phospholipid bilayer mixed with (integral) membrane proteins. The lipid bilayer, therefore, is not a continuous layer, but contains non-lipid molecules, in particular proteins. \n\nThe significance of the model \n\nThe fluid mosaic model is the only accepted proper description of a biological membrane. Its publication in 1972 by Singer and Nicolson (Science 1972 Feb. 18 175:23 720-31) put an end to other existing and competing models of biological membranes. These competing models assumed triple layers, with a lipid core layer and two protein layers, one on each side, but the proteins would not penetrate the lipid core, i.e., did not span the membrane. The fluid mosaic model, based on thermodynamic and functional considerations, postulated instead that proteins as the active components of signal transduction and transport,  must and can span the entire thickness of a lipid bilayer. Protein-lipid interaction was thought to be stabilized through hydrophobic (fat soluble) contact points on the surface of the proteins.  \n\nToday, the fluid-mosaic model is the only accepted model of the structure of biological membranes, including those of animals, plants, and microorganisms (see figure above for membranes of the cell wall of the bacteria Escherichia coli). the use of the same structure among all living organisms is consistent with the idea of a common ancestor organism (or organisms) from which all modern life forms have evolved. Since 1972 no modifications of the model have been necessary, also individual membranes differ in molecular composition, lipid types, or lipid to protein ratios.  \n\nTo understand the stability and form of membranes one needs to understand that they are solely determined by the aggregation behavior of phospholipids. These lipid types in effect form extensive, planar bimolecular films separating two aqueous compartments.  \n\n \n\nThe hydrophobic core of such a thin film functions as an electrical insulator (D=2; D stands for dielectric constant; a value of 2 represents hydrophobic environment, a value of 80 water) allowing diffusion of small hydrophobic solutes across membranes, but inhibits the diffusion of charged and hydrophilic, i.e., water soluble molecules. The incorporation of protein into these bimolecular films provides the necessary pathways for all charged and hydrophilic molecules. Cells have thus complete control over their membrane permeability because they can control protein function, but not the diffusion of small hydrophobic molecules across membranes. Cell membranes are thus clearly specific and selective.  \n\nTo understand the current model of biological membranes, one has to know the history of the study of cell membranes, both from a structural and functional point of view.  \n\n\nEarly developments \n\nThe first experimental indications for a bilayer structure have been obtained by measuring the size of a water surface that can be covered by phospholipids extracted from red blood cells. This area was about twice as large as the total surface area of red blood cells used to extract lipids. It turned out this first calculation was a lucky shot and with more accurate measurement, the two surface ratios would not have been 2:1 due to the presence of membrane proteins. This original finding, although not reproducible, stimulated membrane research and guided it into the right direction.  \n\nNeurobiologist started to characterize the function of neuronal cells by measuring action potentials which are the result of electrical currents flowing across cell membranes (called excitable membranes of nerve and muscle cells). Macroscopic current recordings of isolated nerve cells in the 1940s allowed the prediction of underlying molecular units called ion channels (the electric charges are carried by ions, charged atom
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I'm a biology teacher, and a cell model is a project every year.\n\nUse a HARD beachball about the size of a basketball....or a cheap dimestore basket ball. Cut in in half and use thin wires to suspend the various organelles inside.
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A model of a cytoplasm? Are you sure thet's what you want? Cytoplasm is the stuff that the organelles in the cell float in. I suppose it would be jelly-like. So you could get some clear gelatin, put it in a cell-shaped mould (simple cuboidal epithelia are probably the easiest to shape) and allow it to harden. If you want it to look like a real cell, then stick some organelle-shaped pieces of colored jelly in before the stuff cools.\n\nGood luck!
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You can check out this site, it explains a step by step technique:\n\nhttp://www.pall.com/biosepra/34696_7290.asp
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Search Google and SourceForge :)\n\nRawlyn.
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There's a great tutorial/newsletter on using the RAD Video Tools to do just that.  I've included the link below.  It's a free download and the steps are well explained by PapaJohn.