how we can determine vitamin B1 by HPLC?
469125
A link to a protocol from Alpco Diagnostics:\n\nhttp://www.alpco.com/pdfs/30/30-2201.pdf
272412
Walmart!  Its in the vitamins section and its called Hair and Nail Vitamins.
709812
You can measure it in a number of ways depending on the protein.\n\n-- pH may vary directly with concentration\n-- optical rotation measurements may work for some\n-- adding a dye to the protein and reading on a colorimeter\n-- Another way would be to degrade or react the protein in solution and then to measure the byproducts of the degradation/reaction by something as simple as a titration
614096
The World Without Cancer website has a list of contacts where you can buy B17 from. You must be aware that even though you can buy it in the UK, it is banned in the US. The website also has a FAQ, which you might find useful for more information.
851323
Well, the B12 that they "add back" to cereal because they are processed, is a synthetic form that isn't as easily absorbed by the body...you would have to eat ALOT of cereal and thus probably gain weight and ruin your B vitamin balance...your best option is to go to the health store and buy METHYLCOBALAMIN...it is the ACTIVE form of B12 that will be the best absorbed by the body...I don't eat cereal...All cereals, including organic ones, go thru a machine that alters the natural grains, making them less nutrient dense and taking all the enzymes out so its harder to digest...then companies add synthetic vitamins back...only they don't add all the vitamins that naturally come with the grain.  Stick to whole grains like oats and quinoa.  And get that B12 supplement!
319798
vitamin B has a large content of yellow color. its generates yellow pigment and heat in the body. so your pee turns yellow after taking vitamin B. i feel shy answering this question ofcourse... so i couldnt make many points in public. contact on yahoo messager and i would give a clear note.
507988
Since your question refers to only the types of detectors used in HPLC, I assume that you understand how HPLC works so I will not go into that.  Each chemical refracts light differently.  As the various components elute from the column, a beam of light is passed through the eluted flow.  The components come out as bands of individual components.  Each band or component refracts the light and this refraction is measured by a detector.  The signal from the detector is amplified and sent to a recording device which records the refractive index of that component.  From the refractive Index, the component can be identified and by the strength of the signal, the amount can be determined.  \n\nThe UV/Visible detector simply uses the different wavelengths of light to determine the various components being eluted.  Each component absorbs light rays differently.  The wavelength Can be varied depending on the class of compounds being examined.  This allows for maximum sensitivity of the detector.  As each component is eluted, it passes through the detector.  The absorbence of light gives the identification of the component and the amount of light absorbed is directly related to its concentration.
601120
Vitamin B can make people repellent to bugs.  Check with your doctor and read about it online.
129704
Soo many good supliments out there. \nB-12 (cyanocobalamin) in food is an extrinsic factor, meaning there is B-12 intrinsic factor, located in the stomach, neccessary to make this vitamin usable.\nThat said, I stay away from the subligual B-12 suppliments. They get B-12 into the bloodstream without the need of intrinsic factor, and the body often stops producing what it doesn't need. I haven't heard of this ever having happened with sublingual supplimentation, probably because it would take a while for this to happen, not the usuall couple of months that people usually would try "megadosing" on B-12 with sublingual lounzes. (The need for intrinsic factor limits the amount of B-12 that can be absorbed by the stomach, the average person has enough intrinsic factor to absorb about an 80% RDA B-12 actually). \nLong term vegitarians who don't get B-12 eventially stop producing the intrinsic factor, and then they end up needing B-12 shots for the rest of thier lives, but this is the one and only water soluble vitamin that the body stores, if I remember right, shots are given every 6 months.\nAll that said, of the numerous things I use, Odwalla B Monsters are a tasty source, (I just drank a half). You can drink half, put the other half in the fridge to drink later in the day, and that way have well over your 100% RDA. \nMultivitamins usually have at least 100% RDA, and are cheaper to stock up on than Odwallas, but it might be better to get vitamins from food than pills. A lot of artificial meat on the market is also B-12 fortified, check out the nutritional labels.
1155659
Hello there\n\nVitamins A & B are found in sweet corn. For more info, check out the link below.
860724
The link below gives a method and chromatogram.  Bear in mind that you will need to prevent the degradation of ascorbic acid during the analysis.  This may be done by adding EDTA and HCl to the solutions, but you will have to work out the concentrations.
593464
Vitamin C\n"granduation"
72671
some people swear by B vitamins.
465424
It depends.  If your diet is deficient in B vitamins then a B12 supplement will boost your energy levels.  If you already get enough from your diet then the vitamin supplement will have no effect.  Personally, I find that a B vitamin taken 30 minutes before my morning workout gives me an energy boost.
770873
it is used to preserve food products.in human body (liver) it will convert to succinyl CoA(one of the enzymes that carry out this function need Vitamin B12 as coenzyme) which is krebs cycle intermediate!
784488
Vitamin B is a complex of eight water soluble vitamins, active in cell metabolism. The name arises because it was once considered a single vitamin, much like Vitamin C or Vitamin D. Since later research has shown it is in fact a complex of chemically distinct vitamins that happen to often coexist in the same foods, the name has gradually declined in use, being replaced by the generic term "the B vitamins", the vitamin B complex, or by the specific names of each vitamin.\n\nVitamin B1, (Thiamine)\nVitamin B2, (Riboflavin)\nVitamin B3, also Vitamin P or Vitamin PP (Niacin)\nVitamin B5, (Pantothenic acid)\nVitamin B6, (Pyridoxine and Pyridoxamine)\nVitamin B7, also Vitamin H (Biotin)\nVitamin B9, also Vitamin M and Vitamin B-c (Folic acid) - important for pregnancies\nVitamin B12, (Cyanocobalamin)\n\nOther substances which are very similar in structure and function to the B vitamins have been discovered. Many of them are "essential" vitamins to various plants and animals which cannot synthesize their own. (The adjective "essential" in the context of medicine and nutrition refers to a nutrient being required because it cannot be made by the body. Non-"essential" nutrients are still important.) None of these are "essential" vitamins to humans, because humans can synthesize their own, though some only technically so (choline, for instance, can be metabolized in humans by cannibalizing cells to make use of the choline they contain, killing the cells in the process).\n\nMany of the following substances have been referred to as vitamins because they were believed to be vitamins at one time. Most of them are vitamins with respect to certain plants and animals as well. While they are non-"essential" in that they may be synthesized by the body from other starting materials, they are still important.\n\nVitamin B4 (Adenine) –\nVitamin B7 "Vitamin I" of Centanni E. (1935) — more commonly called Vitamin B7 and also called 'Enteral factor' is a water and alcohol (ethanol & methanol) soluble Rice bran factor which prevents digestive disterbance in Pigions. It governes the anatomical and functional integrity of the intestineal tract. Later (1945) found in Yeast; Inositol, Nicotinic acid, & Biotin are possibles. Note: Carnitine has also been claimed but is not possible as it is insoluble in alcohol. Carnitine as Vitamin B7 was wrongly coped from Vitamin BT.[citation needed]\nVitamin B8 (Ergadenylic acid) – also known as adenosine monophosphate\nVitamin B10 (para-aminobenzoic acid, or PABA)– [1]\nVitamin B11 (Pteryl-hepta-glutamic acid) – Chick growth factor, which is a form of Folic acid. Later found to be one of five folates necessary for humans); (L-carnitine) in France.\nVitamin B13 (Pyrimidinecarboxylic acid) – Also known as Orotic acid, often misspelled erotic acid.\nVitamin B14 – cell proliferant, anti-anemia, rat growth, an antitumor pterin phosphate named by Earl R. Norris (biochemist of folic acid fame) isolated from human urine at 0.33ppm (later in blood), but later abandoned by him as further evidence did not comfirm this. He also clamed this was not Xanthopterin as the French do.\nVitamin B15 (Pangamic acid) – [2]\nVitamin B16 (dimethylgycine) – also known as DMG. (However Lipoic acid was discovered and named a B-Vitamin after B15 and before B17)\nVitamin B17 (Amygdalin) –\nVitamin B18 –\nVitamin B19 –\nVitamin B20 (Carnitine) –\nVitamin B21 –\nVitamin B22 – often claimed as an ingredient of Aloe vera extracts but also in many other foods. Claimed by one source to be Vitamin B12b-delta. first mentioned on internet in reference to Naturopath Linda Clark's book "Know your Nutrition"\nVitamin Bh – another name for(biotin) [3]\nVitamin Bm ("mouse factor") – also used to designate Inositol\nVitamin Bp (Choline) –\nVitamin Bt (L-carnitine) –\nVitamin Bv – a type of B6 but not Pyrodoxine\nVitamin Bw
899650
May be given either as IM or SQ.\nThe needles provided with the B12 and the script will decide how it is given. If IM needles are provided it will be IM. If SQ needles are provided it will be SQ.