what are the procedures for histocompatibility?
365428
Depends if you are doing flow cytometry testing, serology testing or DNA/PCR typing.  If you are doing serolgy testing, then usually you have pre-loaded serum panels on microplates that will be indicative of certain antigens.  You add the patients lymphocytes and incubate.  You then stain using a live dead stain, with fluorescent green indicating live cells (no cross reactivity) or red, indicating dead cells or a rejection reaction.  From these results you can decide which antige3ns the patient would potentially react (or not) with. That's the short answer as there are other lab protocols that encompass HLA testing.
441197
a small volume (1 to 10 ml) of blood is taken, cultured in medium containing a mitogen(to induce WBCs to divide)and placed in a 37 c incubator for about 72 hours. Then a mitosis-arresting agent is added. After treatment with a hypotonic solution like 0.075M KCL(so that the cells will swell and chromosomes spread)the cells are fixed with a fixative (usually made from one part acetic acid to three part methanol)and mixed thoroughly.then a drop of this cell suspension is put on a slide and placed on a hotplate(or in an oven overnight).Harvesting means the step after KCL treatment, when you add fixative.
131060
http://ca.expasy.org/tools/\nhttp://www.biochem.ucl.ac.uk/bsm/biocomp/
163691
For the purpose of "designing" a baby, in vitro fertilization is performed. Then DNA of the obtained embrios is tested, and only embrios that match certain genes of the sibling are implanted. The newborns therefore are genetically very close to their siblings and can donate stem cells, bone marrow etc which will not be rejected by the recipient's organism.
604807
In regards to what? Blood? Sex typing? Genes? Cars? You need to be more specific!!
656205
Try the websites www.protocol-online.com & www.cellculturechat.com\nThe first has protocols for most scientific applications, the second is a forum specifically for cell culture scientists. Good luck!
246421
cloning\nstem cells\ngenetic manipulation
74238
Try the site linked below, "INTRODUCTION/HISTOLOGICAL TECHNIQUE."
717624
Protocols\n\nGood Luck\n\nJimmy
390712
By vaccination for hepatitis A & by believing in God!!!\nwell,\n1)Eat right\n2)Avoid dehydration\n3)Avoid drugs\n4)Try to control itching\nThanks.\nSumiya
278332
I did a google search and found lots of lab write-ups and discussions of research, but not an easy educational source. \nYou might want to try wikibooks.\nHere's a link to one of the searches I did for you.\nhttp://en.wikibooks.org/wiki/Immunology_-_Experimental_Methods_in_Immunology
1108995
getting ready to reproduce
763473
Any doctor or hospital will do it. I remember we did it in biology class in high school. It was a very simple procedure.
1115957
Not with current freezing techniques. Too much cell damage. In the future I think that a process will be possible. Will it be ethical or moral in a rapidly overpopulating world will be the ultimate question.
223927
ANTIBODIES,T C CELLS,T H CELLS,PHAGOCYTOSIS,MHC,CD,COMPLEMENT SYSTEM,APP,CRP,INTERLEUKIN\n,INTERFERON,\nMACROPHAGES,\nNK CELLS,\nCATHELICIDIN,HYPERSENSITIVITY....10 DONE...\nTHERE IS MUCH MORE....BODY IS A BIG TIME FIGHTER...\nINFECTIONS DONOT MESS  WITH IT
479427
Dye-labeled antigens for specific variants of MHC?
561843
The most interesting question of the day, so I had to do some research. While I thought it was impossible because of the antigens present in blood types, this article seems to indicate that it can be done. That sure will relieve blood supply issues.