Does anyone know what is the methodology for doing a Bacteria Culture?
360421
well,a bacterial culture,or microbial culture,is a method of growing a microbial organism to determine what it is,its abundance in the sample being tested,or both.it is one of the primary diagnostic methods of microbiology.it is often used a tool to determine the cause of infectious disease by letting the agent multiply (reproduce) in predetermined media in laboratory.\nthe most common method of microbiological culture uses Petri dishes with a layer of agar-based growth medium in them to grow bacterial cultures.this is generally done inside of an incubator.\nthe term culture can also,though infrequently and informally,be used as a synonym for tissue culture,which involves the growth of cells or tissues explanted from a multi-cellular organism.
593312
I'm not sure what the question is? Are you looking for someone to write an entire grant proposal and post it here? I'm not sure if such a grant would be funded, because the means of preventing culture contamination are pretty well known: Work in a sterile environment (tissue culture hood). Spray everything with ethanol before you put it in the hood. Use disposable pipets, etc.
741317
This link is one of the better microbiology websites that I've used.\n\nhttp://www.textbookofbacteriology.net/
128285
Maybe this will work:  Innoculate the inside of a zip-lock storage bag with your germ specimen. Then seal it and place it in a refrigerator. Scrub hands with betadine sugical scrub for 15 minutes.  Maintain sterile field and technique while putting on sterile gloves.  Keep hands gloved between contacts with the inside of specimen bag, which you will do nine times. Put on a new pair of gloves after each contact, always maintaing sterile precautions.  Hazardous waste management procedures would work well, I think.  Good luck.
916415
Firstly, is the culture tube to be a nutrient broth or a nutrient agar?\n\nWhichever it is, both will have to be autoclaved to remove all other organisms. After autoclaving, don't open until you want to insert the sample. Make sure you have a naked flame in front of you. The area just around the flame is your sterile area. \n\nFor a nutrient agar, heat your innoculating loop in the flame until it is red-hot then remove from the flame and flick it to cool it. Test on one side of the agar to make sure it is not too hot. If too hot, the agar will hiss. Collect your sample of E. coli with the innoculating loop and use your other hand to hold the culture tube. Open the culture tube with as few fingers as possible. Streak the E. coli on the nutrient agar making sure to spread it evenly. Always keep the mouth of the tube near the flame. The innoculating loop should also always be near the flame until the process is complete. Close the culture tube and heat the innoculating loop again.\n\nIf it is nutrient broth, then follow the same steps as above except that you insert the innoculating loop into the broth and shake it. Then close as above.\n\n*Remember... when opening the tubes, always keep the mouth of the tube close to the flame and always sterilise the innoculating loops.*\n\nEDIT: Answer to the replied question.\n\nYeah a micropipette would be easier. You'll need to autoclave the micropipette tips before using and like I said, all the procedures have to be carried out right beside the flame. The mouth of the culture tube has to be in or right beside the flame so that no contamination happens.\n\nIn any case, the innoculating loop or the pipette can be used because the nutrient broth is there for the bacteria to develop colonies and grow so the end result will still be the same.
258097
Is a simple culture enough?\nthe anerobic won't grow in air the aerobic will grow and give off O2. Yeasts will give off CO2.\nYou may need an autoclave to maintain temperature.\n{sorry not to be more helpful}
684712
I'm not sure I understand the question, but I'll try anyway.  :)  If you are trying to determine IF you have both Pseudomonas and Klebsiella on in your culture, you can streak a MacConkey agar plate with the culture.  Pseudomonas cannot ferment lactose, so it will grow on the plate but remain colorless.  On the other hand, Klesiella can ferment lactose, so it will also grow on the plate but turn bright pink.  That's probably the easiest way to distinguish the two from a mixed culture.\n\nAs for the "local disinfectant" part of the question...are you trying to pull out these bacteria and then do disinfectant susceptibility testing on them?  If so, you can streak out the plate as mentioned above, then subculture each so you can get a pure culture.  Then, you do disinfectant tests on the bacteria separately by the disk diffusion method, etc.\n\nI hope that helps some!
1075584
This is a great source for you:\nhttp://www.blackwellpublishing.com/ogunseitan/student.asp\nEnjoy the microbes!\n;-D
76056
take your soil sample and suspend some in a little sterile water; plate out the suspension on agar plates in the usual way and isolate the colonies as a way to do a preliminary sort. Then maybe culture them ... Then do your in-depth analysis.
729453
K.C.'s answer works if you have a selective media that will work. Also (or in addition to that) you may want to plate out colonies and then PCR out a unique sequence or two.\n\nThis wouldn't be definitive but if you design your primers right it may work, but also may be very time consuming.\n\nAlso you may want to try restriction digests to remap your gene. This would work well, if you have a good map.\n\nGood luck, what a bummer
130049
Find a petri dish and some bacteria culture.\n\nSwab your mouth.\n\nRub it across the culture.\n\nLet it sit in the incubator for 24 hours.\n\nAnd voila!\n\nAll the microogranisms in your mouth.
956408
First we have to set up the experiment\n1. Get several pieces of bread 6 or 8 or 12 ?...make them the same size\n2. using an eye dropper put the same amount of milk on them to start the mold (or are you going to let them mold on their own??)\n--make sure they are in an enclosed area that bugs and animals can't get to\n3. once mold is started - put down how many drops of bleach you put on each one\n..for example - \nA. piece - one drop - \nB. piece - two drops\nC. piece - three drops\nof course, if C piece accidentalyy gets 4 drops - swap it for D piece and make D- then C - and put three on the new C (hehehehe)\n4. Now closely watch and monitor your bread\n5. Note which mold was killed - and then you know how much bleach was needed\n...all mold on pieces D E F G H were killed - showing that 4 drops of bleach killed the mold
902971
I'm not sure exactly what you are asking, but I'm assuming you want to know how microbiology is similar to stem cell cultivation.\n\nMicrobiologists use media (agar) that has different types of nutrients embedded into it that promote the growth of the organism they want to isolate.  These plates are placed into incubators that also promote the growth of the organism they are trying to isolate.  Sterile technique is used throughout the process to ensure that the media and specimen is not contaminated.  Colonies with desired characteristics are selected and replated on media several times until only the desired organism is isolated.  It is then tested to determine its species and subspecies before resulting the test and making recommendations.\n\nTissue cultures are cells that have also been plated on media that promote the growth of the desired tissue.  In this case, heart valves.  The innoculated media is then placed into incubators.  Desired tissue is tested and placed on new media in order to create a pure tissue source.  Once the desired tissue is grown, it is tested for the presence of the desired antigens, etc.
668397
Depends if you want to grow pure cultures of microbes or not. If you don't have to have pure cultures, just put your leftovers in the fridge and forget'em. ;-)\n\nIf you want to grow pure cultures, you will need to know aseptic technique and what each type of microbe likes to eat. \n\nSome will go on a defined medium. That is one that you know exactly what it in there. Some have to have something complicated that we don't know exactly what all the components are, like yeast extract, beef heart infusion, that sort of thing.\n\nThen you need to know the temperature and humidity requirements of your bug and any special atmosphere conditions it need, like low oxygen or no oxygen.\n\nIt also helps to know how to store samples of your bug for future use. Can you freeze it? or do you have to lypholyse it?\n\nHope this helps.
1009128
Most of the bacteria that can be cultured represent only a tiny fraction of what's out there. Bacteria exist in practically every niche observable but in general, the best way to cultivate bacteria is to simulate their natural environment. That's why many infectious bacteria are grown on agar (a sugar similar to the ones found in our body) and incubated at 37 degrees celsius (the temperature of the human body)
205493
It's just a bacterial culture, pretty simple at that.
567354
You can get disposable, sterile swabs.  These would probably work the best.  You should be able to get them via online... google "lab equipment swabs".\n\nopen your swab, being careful to not touch the tip.  Run the swab over the section you want to culture.  Then, simply smear the swab onto the petri dish.  \n\nFor the petri dishes, you probably want more than just an empty dish.  You want an agar plate.  An agar plate is a gelatinous material on the bottom of the dish that provides food and nourishment for the bacteria.\n\nYou could probably use gelatin as a stand-in.  Buy plain gelatin and make a batch twice as concentrated as it should be.  This will make the gelatin harder and less likely to melt at room temperature.\n\nGood luck!