how can we improve RNA QUALITY?
373884
RNA are the transporter of DNA. DNA therefore depends a bit on the RNA condition. Both are in the chromosome which condition also depends on the condition of the cells. Cell condition depends on what kind of food and drink you take. The Quality of RNA can be in its best if your body is in good shape and in a good biological condition. Take good food, balanced diet and best of all take plenty of water cause it is the primary component of everything inside.
873476
Hi friend,\nSince your question is a bit technical, i assume that you are from a microbiology/biochemistry background. Hence the answer too is given with technical details. If you can't digest it, i may be able to give a simpler explanation. Just leave a comment alongside the answer.\nIt is also assumed that ur querry is about rRNA differences between EUBACTERIA and Archaea.\n\nGenerally speaking, unlike Bacterial RNA, archaean  RNAs are not capable of catalysis in the absence of other enzyme components. The structural basis of this deficiency must be in the  differences in  bacterial and archaeal sequences or higher order structure. \n    Secondary structure in Archean RNA contains all but one helix present in that of Bacterial structure, the 2 bp helix P11. This structure is adjacent to a large region in which the structure remains unknown even in bacterial RNase P RNAs. \n\n           In Eubacteria, rRNA modification is achieved exclusively by site-specific enzymes while in archaea and eukaryotes the formation of 2-O-methylriboses and pseudouridines is guided by numerous snoRNA that direct a catalytic machinery to the target sites on the pre-rRNA.        \n         \nMore over the GC content of the paired stem regions of the 16S rRNA genes in Archaea is very high and it correlates somewhat with the high  temperatureconditions they grow (or evolved in)\n\nThat's what i know of the differences. Hope thats clear,\nBye..
961312
Well, that is the Holy Grail of biochemistry and we've been dreaming of that ever since Dr. Pauling had me researching metabolic diseases, Lesch-Nyhan syndrome in particular.  The problem has been 1) finding the precise genetic coding of the fouled up part of the DNA and 2) devising a mechanism to clip out that nucleic base and replace it with the correct one (or series of bases).  Precision is, of course, vital or you'll kill the experimental animal (or, ultimately, human patient).
781789
mRNA is the RNA that carries information during transcription and translation. It has codons, which match up with the anticodons on tRNA. tRNA is the RNA that bonds to amino acids and transfers them to ribosomes, and mRNA. The reason RNA is used is because the cytoplasm is too damaging to DNA, so RNA is kind of a scapegoat. Hopefully, this helps.
1140466
Improve your spelling.
643728
http://www.hr-rna.com/RNA/Spider%20pages/Green%20Lynx%20page.htm\n\nhttp://www.biosurvey.ou.edu/okwild/misc/glspider.html
498173
Yes I have worked with RNA before. The main difficulty with all of the procedures is making sure you have gotten rid of the RNAses. They are very tough and stable, so you have to make sure everything has RNAse inhibitors (DEPC). I dont know enought about guinea fowl to know if they use similar polyA tails that humans, etc do. If not, the oligo-dT resin wouldnt have much to bind to. I dont know what your goal is but you could consider a PCR strategy to amplify what you are looking for. If you are looking for some tissue expression, you could look into buying premade mRNA from a company, which we used successfully before. hope that helps!
8687
mRNA  \nCapped messenger RNA is produced directly from your plasmid that contains a T7 or SP6 promoter. It is purified by our proprietary PUREmessenger™ technology. PUREmessenger™ is a chromatography that not only eliminates traces of DNA and proteins but also allows the selective recovery of the mRNA of the correct size. Shorter abortive transcripts as well as longer contaminating mRNA that are produced during in vitro transcription are eliminated. Capped mRNA will be purified by PUREmessenger™, quantified, re-suspended at 1mg/ml (except if otherwise specified) and aliquoted at 50 µl/aliquot (except if otherwise specified) in sterile conditions. It will be sent to you on cooling blocks together with its documentation: formaldehyde/agarose gel documentation picture. The capped mRNA that is sent to you ready-to-use (dissolved in water or the buffer of your choice) is 100% of your mRNA molecule of interest.\n\nCapped mRNA is produced in clean room facilities for research or clinical applications.\nYour benefit\nHighly purified, efficiently capped mRNA that has a superior efficacy compared to standard mRNA produced with in vitro transcription kits and simply purified by precipitation thus contaminated with alternative transcripts and traces of proteins and DNA fragments. The high quality of mRNA is reproduced from batch to batch. This guarantees the consistency of your results. Capped mRNA can be produced for research or for clinical utilisation: "documented" to be used as Active Pharmaceutical Ingredient or GMP-certified.
109472
Let's start with something simple.\n\nIf you can't even undertsand how to write complete sentences and employ basic punctuation what makes you think you will understand anything about nucleotide sequemces?
1026036
Degradation rates of t-rnas in blood stains in different environments.  Crime investigation applications are endless.
592029
rna is copy of the dna used in transcription (i think) then it's translated back into dna somewhere else in the cell
653085
RNA is necessary for protein synthesis.  I would expect that the cells that have the highest rate of protein synthesis, (rate of amino acid addition, as opposed to rate of protein completion would be a better metric) to have more RNA out in the cytoplasm doing its thang.\n\nThe granules  in granulocytes are chock full of enzymes (a kind of protein) for digesting mean ol' invading microorganisms. What I don't know is if the enzymes are constantly being sythesized anew within each granulocyte, or if they are produced in a burst when the granulocyte first forms.  In the latter case, I would not expect much difference in RNA concentrations except maybe when they the cells are new.  In the former I would expect more in the granulocyte. \n\nI know next to nothing about white blood cell formation, so I can't say more than this.
803356
As you know, mRNA has copied the protein-making instructions from the DNA. Well, tRNA brings the required amino acids to build the primary structure of the protein, as is stated by the instructions on the mRNA.
1171167
Try this , it really helped me in my first few lectures in cell bio , such as DNA and RNA . Loads of animations and stuff ! \n\nhttp://www.johnkyrk.com/DNAtranscription.html
602170
RNA is short hand for Ribonucleic Acid.  RNA has a similar structural appearance to DNA (Deoxyribonucleic Acid).  You can read about the chemical structure at the site referenced below. \n\nRNA crops up in several different cell locales from the nucleus to the ribosomes and appears to serve in a variety of cell roles.  The basic function most often noted is that of a messenger or transfer template upon which proteins are built.  The information for this construction comes from the DNA which unzips long enough for these RNA templates to be formed. \n\nThis is seriously cooked down discussion of a key chemical player in cellular dance of life.  If you want to know more, I suggest a visit to your local library.  The subject area you want is biochemistry or molecular biology.
198401
ummm... let's see.... the transcription of RNA means that it is it's encoding, or if you want to call it, program. It is the set of chemical codes used for producing DNA. Translation, on the other hand, I am not still not quite sure of. I believe that it might be the refined form of RNA, like trying to create a vaccine for somthing. I hope this helps!
676152
All the programs listed here work well, but if you want higher quality you might just try turning up the quality in Windows Media Player.