How low pH phenol do we need to put into TRI reagent for RNA extraction ?
359472
Tri reagent is a mono-phase solution containing phenol and guanidine thiocyanate.  For RNA extraction, the pH of phenol should be 4 (which helps keep DNA in the interphase following chloroform addition).  Most manufacturers sell pre-mixed Tri reagent containing phenol, so it is likely that you will not need to add additional phenol to your solution.  Check this first.\n\nFor extraction of RNA from plant cells, your method of homogenization may be important and you may need to remove insoluble material by centrifugation prior to phase separation with chloroform.  Also, this is messy stuff and phenol is very dangerous, so I recommend adding "phase-lock gel" to your tube (made by Eppendorf, purchase via Sigma or VWR) to physically separate the organic (phenol) layer from the aqueous layer containing your RNA.
449926
The molecular formula is C6H5NO5P\n\nStructure (if in case you want it too) is as follows:\n\nDraw a benzene ring (phenyl group) minus 1 hydrogen (C6H5). To the Carbon without the hydrogen, attach a Hydroxyl group (-OH). \n\nOn the other Carbon positions, whether it is the ortho positions, meta positions, or para position, erase the hydrogen and attach a nitrate group (-NO2). \n\nOn the Oxygen with the double bond to Nitrogen on the Nitrate group, erase one of the bonds and attach this bond to the phosphate group (-PO3).\n\n4-nitrophenol phosphate is used in a substrate for a timed test for alkaline phosphatase turning colors for 4-nitrophenol
589633
1)there are 5 to 9 nucleotides on 5' mRNA named Shine Delgarno which are the match for 16SrRNA on 3' in the small subunit of ribosome.so 16SRNA conduct ribosome to AUG by binding to shine....and helps to initiate the protein synthesis.\n\n2)there are special sites on 16SrRNA which have a direct reaction with antisense on tRNA\n\n3)it binds to IF3(initiation factor3)
1122499
pH = pKa - log (.25/.4) ~= 4.3
532375
Phosphoric Acid, H3PO4.
471742
Ummm...You will have to figure out which is faster... but I can give an idea as to proceedure...First, try to answer this question: What are all the colored species in grape juice? You need to know that first. Hopefully there is only one. That would simplfy things. Then you need an extinction coefficient for that compound, if you can find one. Using that, you can take several absorbance measures and use Beer's law to establish the concentration of that species in your stock solution. Beer's law states that Absorbance = extinction coefficient x concentration x path length. Your path length is the width of your cuvette, which is usually 1 cm. Beer's Law will not be valid if you use a solution that is so concentrated that it gives you an absorbance value greater than 2. At Absorbance = 2, well over 99% of light is being absorbed. A reading over 2 is thus considered analytically unusable. Your juice can be diluted, if necessary, with DEIONIZED (aka distilled) water. Once you do that, I would roughly time each reaction by just looking at it with my eye, adding just a drop or so of bleach/peroxide initially, and increasing the amount only if necessary. Make sure to add exactly the same amount of bleach/peroxide to exactly the same volume of grape juice. Once you have the reaction basically set up to your satisfaction, then place your juice sample in the colorimeter and get ready to add your reagent. Remember to take a measurement before you add any bleach/peroxide for EACH of the two tests. And take your measurements on the same time frame (ex: every 30 sec.)Good Luck!!! :-)
574078
This might help :)\nhttp://www.chemicalforums.com/index.php?board=8;action=display;threadid=3263
367522
For pH3; Citrate buffer (Sodium citrate/citric acid) \n\nFor pH 8.75; Borate buffer (sodium tetraborate/boric acid), or TRIS [tris(hydroxymethyl)aminomethane]\n\nFor pH9.3; borate buffer, or carbonate buffer (carbonate/bicarbonate)
793817
Phenol.  If a proton attaches to the oxygen in Phenol, the positive charge can be stabilized in the aromatic ring by resonance.
172269
take an epindorph tube, place about 500 microlitres of blood, centrifuge, remove supernatent, wash with PBS buffer, repeat 3x, by an RNA isolation kit from roche and foolow the instruction!!
498173
Yes I have worked with RNA before. The main difficulty with all of the procedures is making sure you have gotten rid of the RNAses. They are very tough and stable, so you have to make sure everything has RNAse inhibitors (DEPC). I dont know enought about guinea fowl to know if they use similar polyA tails that humans, etc do. If not, the oligo-dT resin wouldnt have much to bind to. I dont know what your goal is but you could consider a PCR strategy to amplify what you are looking for. If you are looking for some tissue expression, you could look into buying premade mRNA from a company, which we used successfully before. hope that helps!
898647
The greater acidity of phenols is due to the stability of the phenoxide ion which is resonsnce stabilized.\nPhenoxide ion is a benzene ring with (O-) attached to it.( Note that this Oxygen atom has 3 lone pairs)\nyou get 5 resonance structures for phenoxide ion.\nMore the no of resonance strucutres, more resonance stabilized it is.\nwhereas in alcohols atmost 2 resonance structures only are possible which accounts for its less stability and hence less acidity.
853918
Tris buffers the solution at a given pH.  If it's not buffered, the tendency is for the buffer to become acid as the gel progresses.  If the pH becomes acidic, the gel looks like garbage.\n\nEDTA is a chelating agent, it will protect the DNA from the action of DNAses.\n\nBorate provides ions to the solution to provide for optimum conductance of the electric charge while the gel runs.
583395
hmmm! go to this site>>>\n Candidate Reagents for Dissolution of Hanford Site Tank Sludges ...File Format: PDF/Adobe Acrobat - View as HTML\nIn none of the 5 runs with these compounds was the complete dissolution of ... acidic or moderately alkaline media without perturbing the oxidation state of ...\n \n  >>>> www.pnl.gov/main/publications/ external/technical_reports/PNNL-14378.pdf
781203
2H3PO4(aq) + 3Mn(OH)2(s)  --> Mn3(PO4)2(s) + 6H2O(l)
745545
these might help..\n\nhttp://www.blackwell-synergy.com/doi/abs/10.1111/j.1365-2621.2006.01347.x\n\nhttp://www.co-lipidology.com/pt/re/colipidology/abstract.00041433-200502000-00009.htm;jsessionid=Fh8fr0yV02g1pkF1LGYLXyzy9lPXZ4Gn69QZKHJ1GG1vJYGw1czy!-1640309041!-949856145!8091!-1\n\nhttp://scholar.google.com/scholar?hl=en&lr=&q=cache:yYCPrA4fU54J:bmc.ub.uni-potsdam.de/cgi-bin/show.pl%3F1475-2891-4-33/1475-2891-4-33.pdf+list+of++biochem+experiments,+TEAC+assay+or+the+phenolics+test
889419
1) Decide what solvent you want to use: water or organic?\n2) Decide how much solution you want: 1L?\n3) Check the solubility of phenol in the solvent you choose. And calculate how much phenol you'll need by weight to make a saturated solution\n4) Find out the relationship between solubility and temperature.\n5) I assume you want to make a saturated phenol solution at room temperature. If the solubility increases as temperature goes higher, add a bit more phenol than needed (from the calculation above) to the solvent. \n6) Heat the flask or beaker that contains the solvent and phenol until all phenol dissolves.\n7) Let the container cool down to room temperature.\n8) If no phenol crystalizes the solution is over-saturated. You may rub the side of the flask or beaker to help phenol crystalize.\n9) You have your saturated phenol solution!!