what is step of treatmenr scid gene therapy?
371540
The doctors isolate blood stem cells from the bone marrow.  They treat the cells with a retroviral vector containing the normal gene for the γc interleukin receptor subunit, and return the treated cells to each donor.
462921
Sure it WOULD work if there were means to deliver it to each cell, ensure 100% restriction efficiency & specificity, and ligate the DNA nick afterwards. So far it is sci-fi; prenatal diagnostics is the way to go for now.
683631
The problem with AIDs is that it requires complete gene therapy.  All of the DNA that has been changed by the disease has to be fixed.  That is pretty tough!  It takes years just to fix one strand of DNA from cancer.  The best thing they can do right now is drugs, to trick the DNA and slow down the process.
191938
pcr, gene therapy, antisense drugs... umm
765843
There is a very cool way to demonstrate the S phase of the cell cycle.  There is a chemical compound BrdU (Bromodioxy uridine) that can act as a replacement for genetic thymadine.  If you inject the animal undergoing S phase with Brdu, it will be incorporated into the replicated DNA.  You can then label that chemical with either Immunofluorescence or Immunohistochemical reagents that will either make the label visible because it fluoresces under specific light conditions or will stain a dark color and be visible in a light microscope.
984896
Center for Regenerative Biology - maybe??\n-MM
394349
This article might help if you reproduce their test system:\nhttp://www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=Retrieve&db=PubMed&list_uids=15159161&dopt=Abstract
718916
◙ use this tool http://cit.ucsf.edu/embedmedia/step1.php
13361
Cystic fibrosis is an the most common autosomal recessive condition in caucasian children.  It has an occurrence of 1/2000 and 1/22 are carriers in the caucasian population.  It is characterized by abnormally elevated sweat chloride levels, pancreatic insufficiency in 85% of patients and blockage of the airway by thick, heavy mucus.  The later contributes to chronic airway infections.  Half the patients die before the age of 30.\n\nThe gene responsible, CFTR on chromosome 7q31 encodes for a chloride ion channel.  A 3-base-pair deletion in the first nucleotide binding domain is a typical cause for the disorder.  \n\nRecent gene therapy trials have attempted in vivo GAT using recombinant adenoviruses or liposomes to deliver the CFTR gene to the respiratory epithelium have been attempted.  The spice tumeric has also been indicated as potentially limiting the severity of the disease.  Finally, liver transplantation may be a possibility.
692973
There are only two broad methods for gene therapy. I believe what you are looking for are the two main methods of somatic gene therapy.\n\nSomatic gene therapy can be broadly split in to two categories: ex vivo (where cells are modified outside the body and then transplanted back in again) and in vivo (where genes are changed in cells still in the body.)\n\nSo technically speaking the three you are looking for are:\n\n1. Germline Gene Therapy\n2. Somatic In Vivo Gene Therapy\n3. Somatic Ex Vivo Gene Therapy\n\nFor clarification of the response below mine there are actually four gene therapy vectors:\n\n1. Retroviruses (such as HIV)\n2. Adenoviruses\n3. Adeno-associated viruses\n4. Herpes simplex viruses
949412
Yes, try to this!\n\nhttp://www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=Retrieve&db=PubMed&dopt=Abstract&list_uids=3906008
1023535
http://www.wcmc.org.uk/biodev/reports/guiding_principles.pdf
606876
talk to your doctor for treatment.
820948
You might want to read this\n\nhttp://www.patient.co.uk/showdoc/23069180/\n\nthe treatment part is towards the bottom of the page
172269
take an epindorph tube, place about 500 microlitres of blood, centrifuge, remove supernatent, wash with PBS buffer, repeat 3x, by an RNA isolation kit from roche and foolow the instruction!!
616031
No it has not.  I would imagine even if we know the location of the gene (I bet its multiple genes) it often does not target treatment...yet.  There are a number of diseases that have been mapped but the treatments have not been affected.  Its one thing to know the where.  It is an entirely different thing to know the how.  \n\nOne day I would hope we might even be able to insert recombinate DNA to cure or treat these disease processes.
16728
The only way to do that would be either Gene therapy or to wait for a mutation causing more receptor sites on the cell membrane of the cell