what do 2 barely basline resolved peaks say about an LC elution to separate 2 unknown acids?
151863
Either:\n\n(a) you're not getting a very clean separation, as the peaks should be vivid\n\nor\n\n(b) your detector is insuitable for the acids you're studying.\n\nAtleast, that's my analysis. Any A-Chem experts here?
125296
use spectrophotometric method(uv-vis spectrometry).Few things u shud remember.....\n1) Use Labert-Beer's law\n2) u shud know the molar extingtion coefficient value of that particular species\n3) concentration of the species\n4) Calculate lambdamax. using LB law\nIt is an indirect method.\n\nUV-VIS. Spectroscopy will give u the lambdamax value directly...alongwith absorbance
480845
The two should be separable by HPLC
908306
shouldn't you be doing your homework? all you need is the normal ranges for the pH, PCO2, PaO2, and bicarb to interpret this.
495941
We have no idea what data you [we] have to work with here.\n\nWhat did you do in your lab?\n\nI assume you had a saturated solution of Ca(OH)2 which you then titrated with a stron acid like HCl.  You then should have recored the volume and concentration of the acid required to neutralize the Calcium Hydroxide.\n\nWrite out the chemical reaction between Ca(OH)2 and your acid, find the mole ratio between the moles of acid used and the moles of OH- in solution, and solve.
774155
This dissociates into Cl- and\n(CH3)2NH2+\n\nCl- is an extremely weak base, so weak in fact that it is neutral\n\n(CH3)2NH2+ on the other hand is a regular weak acid that will make the solution acidic.
409824
When what is mixed is not fully disolved into the liquid medium.  It usually settles.  A cenrifuge will not separate out the components that are not part of the solution.
302188
The amino and carboxyl protons are very difficult to observe. Using 100% D2O will replace the carboxyl and amino protons with 2H, and you won't see anything. Use a different solvent and you can't be sure you are in the zwitterion form (pH is difficult to control in non-aqueous solutions and you will get different hydrogen bonding)\n\nIf you really want to see those protons your best bet would be to use 90% H2O / 10% D2O with either phosphate buffer or the perdeuterated buffer of your choice (you can get these from Cambridge Isotopes). Make sure the buffer concentration is large compared to your amino acid, and make sure you have adjusted the pH to the zwitterion range. When you aquire the spectrum you will need to use a water suppression sequence. Presat is the easiest, although I much prefer gradient selected 3-9-19. This still may not be enough to see the protons-- if they are exchanging the linewidth may become so broad as to disappear into the baseline.\n\nWhile it's difficult to observe the carboxyl and amino protons (they are *very* sensitive to solvent and pH) you should be able to see changes in the chemical shifts of the remaining protons, particularly the alpha protons.\n\nIf you are really interested, make a sample of your favorite amino acid and take spectra over a range of pH. Plot the chemical shifts of the visible protons as a function of pH and the shape of the curve should allow you to determine the pKa of the amino and carboxyl groups. Here you could get away with just using D2O, which makes the spectroscopy much simpler.\n\nGood luck and have some fun with this!
379862
each of the acids that you gave completely separate (or dissociate) into two ions.\n\nKCl -> K+ + Cl-\nAgCl -> Ag+ + Cl-\nHF -> H+ + F-\nHNO3 -> H+ + NO3-\n\nKCl, and HNO3 all dissociate completely in solution, so therefore they are strong electrolytes. \n\nHF is a weak acid, therefore is a weak electrolyte.\nAgCl is not completely soluble in water, so is therefore a weak electrolyte.
639146
It will be the pH of the sodium hypochlorite formed.  Determine that amount, look up the Ka of the acid and solve.
1024314
well, not an analytical fan myself, but here is what I know\n\n1) one strong and board absorption bend at 3650-3200 cm^-1 indicates -OH functional group\n\n2) No peaks just above 3000 cm^-1, no sp2 carbon\n\n3) peaks just right of 3000 cm^-1 indicates saturated carbon chain\n\n4) one peak, sharp, right of 1400 cm^-1 indicates methyl group.
840473
Need more specifics as to your problem.\n\nHowever, I assume you are trying to do a quantitation on a UV or vis absorbing substance.\n\nMake up  a set of standards of known concentration of your analyte in the solvent you will be analyzing in.  Then do some scans over the whole wavelength range. \n\nUsually pick the wavelength at the top of the largest absorbance peak, unless that is a wavelength which will have interferences from other substances present.  If so, then pick a wavelength with good absorbance that is not subject to such interference.
441718
Key points to look for include:\n\nInfrared\n-NO2 bonds: 1515-1560, 1345-1385\nAlkenes: C-H: 3020-3080 (moderate), 675-1000\n         C=C: 1640-1680(variable) \nIf wet: Large OH hump in the 3500 area... should obliterate most other peaks in the area.\n\nI am not as up to scratch with NMR.\n\nThis is a common compound and an IR and NMR spectra should be easily found on the internet. I recommend chaecking them carefully as jus tlooking for the peaks above will probably not be highly useful.\n\nEven better than the internet is a resource book, any laboratory or university library should have such resource books.\n\nPersonally I recommend that if you are doing much organic chemistry to get 'Organic CHemistry' by Morrison R.T. and Boyd R.N., published by Prentice Hall.\nThis book has an excellent guide for spectra.
1118024
Acidic \n\nbelow 6 acidic above 7 basic
860724
The link below gives a method and chromatogram.  Bear in mind that you will need to prevent the degradation of ascorbic acid during the analysis.  This may be done by adding EDTA and HCl to the solutions, but you will have to work out the concentrations.
703155
No surprise at all, the transition does not take place at an pH of exactly 7 but already before. Take a look here for a graph:\n\nhttp://antoine.frostburg.edu/chem/senese/101/acidbase/faq/indicating-strong-or-weak.shtml
235402
See comment above.\n\nYou do need to do several trials at various concentrations though. You can then determine it through a linear regression.\n\nI also suggest that you consult a book on Analytical chemistry, because Beer's law has limitations. For example, it loses linearity at higher concentrations.