extract DNA from an onion and justify its protocol?
150996
remove outer skin, peel of a layer, pulverise it in a pestle and mortar, actually look at the website, its a really good explanation!
542747
It depends on how long the lysate was thawed...anything over 5-10 minutes and I would throw it out.  Also, if it's thawed/frozen again more than once, I would throw it out.  If you were going to extract DNA it wouldn't be so critical, but with RNA...well, you know how fragile it is!!
855215
Sure.  You say that the cells are competent.\n\nJust clone your cDNAs into your plasmids, and then perform transformation (chemical or electrical, your choice).\n\nElectrical transformation's more fun, though I have caught a specimen on fire that way.\n\nBacteria aren't picky about which plasmids they intake, just make sure that the plasmid contains a bacterial replication promoter so that the bacteria knows to duplicate it while performing mitosis.\n\nAlso, plasmids in excess of 12kb length have trouble getting into bacteria, so be warned.\n\nHope this helps.
1102163
For ssDNA, a dimer (a two base sequence) is the answer...this would be the smallest DNA sequence possible from a PCR reaction. The organic extraction part is not the limiting factor until the number of bases in the sequence becomes very large. Extraction regime should be desalting, followed by Ethanol precipitation, and then either Gel electrophoresis or Liquid Chromatography.
355375
Go here. http://www.dnacenter.com/\n\nYou can't make them get it done, but you can trick them into giving you DNA by collecting a straw they have used or a cigarette butt or, obviously, a semen sample. Anything with hair, skin cells, or spit will also work. There is no age or time limit.
372921
http://faculty.clintoncc.suny.edu/faculty/Michael.Gregory/files/Bio%20101/Bio%20101%20Lectures/DNA/dna.htm
540271
Investigate cloning. Clone some plants
393656
You have to send in a sample.  You wouldn't have to travel personally to a lab.  I'm assuming you mean some sort of DNA fingerprinting profile.  We're a long way from mail-in complete genomes.
1133910
DNA has already been cloned, so your question is meaningless. This was done in bacteria over 40 years ago, and is how human insulin is now produced en masse.
320780
At this point in time it isn't really possible.  in theory it is though.  we can manipulate chromosomes in bacteria and viruses and engineer plants to contain more vitamins or be bacteria resistant but humans are different.  tampering with humans brings up a whole array of ethical questions.  If we were to 'engineer' humans to be faster, smarter and such we would have to select for those traits we want from people who possess these qualities and 'insert' that trait into the chromosome of the subject.  in theory we could do much more with this technology.  Currently this technology is a near vision at best.  \n\n~Chris~
702124
You can find practical information from Qiagen website if you use their kits which are quite popular these days.  \n\nIt depends on which methods you use for isolation.  The alkali lysis (see link below) was commonly used to rupture bacteria.  Plasmid DNA (smaller in size and more soluble) is separated from baterial genomic DNA (larger and less soluble).  The plasmid DNA is then separate from proteins by phenol extraction where the proteins are removed to the phenol phase while plasmid DNA stays in the aquous phase.  The plasmid DNA is then precipated with ethanol to complete the purification.\n\nQiagen's kit and others overcome the use of phenol (which is toxic and smelly) by using a solid phase that bind DNA to seperate the contaminating proteins.  The DNA is later eluted from the solid phase to complete the purification.\n\nHere are additional links:\nhttp://biotech.biology.arizona.edu/labs/DNA_isolation_plasmid.html\nThe concept of alkali lysis method of plasmid DNA isolation is shown here:\nhttp://a32.lehman.cuny.edu/molbio_course/plasmid_protocol.htm
842192
Is this from The Onion?
1136128
No its impossible. The DNA is completely different, and plus cloning was banned by the Geneva Convention a couple of years ago I believe. But if your like the other groups and you want to try it, have at it. But if you do clone it together, dont tell the press or you might just end up going to prison.
132431
You may need watson - creek structure\nDNA structure
882103
Ok, what I normally do is to digest the plasmid with RE, digesting as much as possible for long time (even over-night). Then, without any purification I treat my digestion with CAP (calf alkaline phosphatase) for one hour at 37C. This phosphatase is generally versatile and works in any buffer, but you might want to check with the manufacturer's instructions for buffer compatibility.\nAfter de-phosphorylation, I make a 1% agarose in TAE with a comb with big wells. If you do not have one, you can tape 2 "teeth" together to make a bigger well. Make sure you add Ethidium Bromide to the gel. I run all my digest on the gel for 1 hour or so at medium volt (so that the gel does not become hot) and then cut the band  and purify with a gel purification kit (Qiagen has a very good one). The kit has columns that bind DNA from which your plasmid can be eluted - If you don't have a lot, elute with a small volume of H2O (DNAse-free!).\n\nThe advantage of running the gel and purifying the band is that you get rid of all un-digested plasmid which would be useless in the ligation but that would definitely be transformed into your E.coli (quite well, as well!).\nAlso, if you are cutting and purifying an insert from a different plasmid, you can run vector and insert side by side to see in which proportion to organise your ligation. If you don't have a lot material, try "Rapid Ligation Kit" by Roche. It works at room temperature within 1 hour.\n\nHope this helps!\nGood luck
91264
deoxyribonucleic acid-but you dont really need to know that
1147606
http://biotech.icmb.utexas.edu/search/dict-search.html