How would you use sequence info for a a gene in Drosophila to identify/localize a similar gene from a mouse?
145915
Stick the sequences in a computer and tell it to look for stretches of a certain length where a certain percent of the nucleotides match.  How close a match and how long a sequence depends on exactly what you're looking for.
844734
mRNA transcripts carry the genetic information of specific genes to the ribosomes where they are translated into polypeptide sequences which later become proteins.
452878
From my understanding tDNA is important, at very least for tagging but I believe it is also important for the transfer of the intesert into the A. thaliana genome.\n\nYou can still detect the insertion of the transgene because you would usually insert an antibiotic resistance gene with the gene of interest. You can then use this to select for plants that have the gene inserted.\n\nThen, to find out where in the genome the transgene has inserted, you need to map it. There are many techniques for doing this, but if you know the sequence of your gene of interest or the antibiotic resistance gene, you should be able to use fragments of the genes as probes on gene libraries etc etc etc, there are probably also fancy ways you could use genomic to achieve this too.
230683
I respectfully disagree with the previous answer.\n\nGranted, X-Ray crystallography was used to discover the structure of DNA, but it was not used to sequence DNA.  Educated guesswork would not come close to sequence some billions of base pairs.  What is used is a "shotgun" approach, developed by Craig Venter (I hope I spelled that right) of Celera Genomics.  Essentially, it sequences random fragments of DNA, and a computer puts these fragments together into a sequence.  \n\n1. Between two random humans, ~99.9% of their DNA sequence will align.  Aside from the fact that sequencing one person's DNA would be impractical, one person probably wouldn't represent a "human" genome particularly well.  A group of volunteers was actually used in the Human Genome Project.  In other words, the current sequence used today is not from one person, but from many people.\n\n2. The genome was sequenced not as a random series of G's, A's, C's, and T's (I guess in a way it was), but it was sequenced with the intent to study various functional units --genes.  Funnily enough, most people's genes are pretty much the same.  Even more odd is the fact that a human gene that codes for a particular protein is typically nearly identical to homologous genes in other species.  Some proteins that we have even have homologies with bacteria and plants!  (Conclusion: DNA sequences between humans, and even between species, like chimps, are really really similar)\n\n3. This is not to say that everyone's DNA are exactly identical, but they are close enough where groups of volunteers could be used.     \n\n4. Just in case you're curious, even though our DNA and a chimp's DNA are almost identical, I don't know about you, but I'm not a chimp.  The reason for this (and the focus of much biology research today) is how our genes are regulated versus how a chimp's genes are regulated.  \n\nSorry for the long answer, but I really like this stuff.  It's what I do.
135811
gene I think is what you are looking for. Not sure the question makes sence.
1035639
Well the size of the gene wont help us much to identify your PCR product. It all depends where your primers are located. You can get the rough sequence at the ncbi's website and align you primers with it so you know the size of your amplicon.
1134584
Here you can find.\n\nhttp://www.bioinformatics-india.com/public/drupal/index.php\n\nIf you need more info, feel free to contact me.
729453
K.C.'s answer works if you have a selective media that will work. Also (or in addition to that) you may want to plate out colonies and then PCR out a unique sequence or two.\n\nThis wouldn't be definitive but if you design your primers right it may work, but also may be very time consuming.\n\nAlso you may want to try restriction digests to remap your gene. This would work well, if you have a good map.\n\nGood luck, what a bummer
413968
http://dev.biologists.org/cgi/content/full/130/17/4123?ck=nck
698858
FIrst, using redundant coding, you find the gene by using redundant PCR primers which have the codons coding for the amino acid sequence.  Actually, nowadays, because the human genome is known, you can input the amino acid sequence to the NIH site (and others) and the codon sequence (and gene) which codes for that protein will be determined.  You use specific primers to pull out copies of the gene, and insert them into bacterial vectors- insert coding vectors with the gene into bacteria, and allow the bacteria to produce it in quantity-  Purify it through HPLC, and there you go.
6224
Sequence profiling tools in bioinformatics refer to all those software tools (web-based/downloadable) that provide a brief overview on all related information about an input sequence.\n\nThe advent of post-genomics era has given rise to a range of web based tools and software to handle the deluge of biological information. A simple web-search returns any number of such services and software tools ranging from the popular public domains e.g. NCBI, ExPASy, Ensembl, PDB etc to private research group sites e.g. eF-site, Dipole moment server, SuperPose, REBASE. However, most of these remain obscure/under-explored to a bench-top molecular biologist who most often would like to quickly know which record in the BLAST report is useful for further analysis (given that the first few hits are already known) OR like to have a guess at what would a newly found ORF look like after translation.
441727
These might help-\nhttp://www.itools.com/research/\nhttp://www.mun.ca/biology/scarr/2900_Species.htm\nhttp://www.biocourse.com/mhhe/bcc/domains/olc_toc.xsp
614425
youve got promoters, enhancers, introns and exons, insulators, terminators..is that what you were asking?
940086
Hi:\n\nConserved sequences are those segments of the genome that vary little or not at all over different species, genera, or classes that help us identify the particular organism. For example, all bacteria have conserved sequences that are conserved only among bacteria. Within bacteria, you have sequences that are specific to particular genera, say Pseudomonas has specific sequences that is specific only to Pseudomonas and not to Bacillus, or Staphylococcus. Within Pseudomonas aeruginosa, for example, sequeces are conserved that is not found in, say, Pseudomonas fluorescens. 16s rRNA is one such example. This has been conserved among organisms and the DNA sequences are so different that they hold taxonomic value.\n\nConsensus sequences, on the other hand, are related to gene expression. They are specific sequences, usually a few bases to a few hundred, that serve as recognition sites for the transcription enzymes identify the start of a gene for transcription. This includes the promoter region found a few tens of bases before (upstream) of the start codon (ATG). One common example in prokaryotes is the TATAAT box, also called the TATA box, present a few bases before the transcription start. This has nothing to do with classification of the organisms.\n\nHope this helps.\nCheers!!
931137
It really depends on the lab.  In my lab, they extract DNA and run markers to find what genes the animal has.  In other labs, you may find the actual sequence of the DNA (A-C-T-G).  Genetics is a large field and no two labs do the same thing.
810303
Observing the Development\nof Drosophila in Apple Juice Agar\n--> http://www.accessexcellence.org/AE/AEPC/WWC/1994/observing.html
957887
Each chromosome in your body has an "identical pair"- there are two chromosomes that code for the same functions- one from your mother and one from your father.\n\nBecause they come from two sources, they are coding for the same functions, but may code differently for them (ie blue eyes as opposed to brown eyes).\n\nDuring prophase one, each chromosome duplicates into two sister chromatids joined by a centromere, and then the double stranded chromosome finds it's partner which is coding for the same thing (just came from the opposite parent), and is also double stranded.  Now you have a 4 stranded tetrad.\n\nGenetic variability comes from crossing over.  This is where the two chromatids closest together in the tetrad exchange some genetic information.\n\nFor example, two of the chromatids code for blue eyes, brown hair and shortsightedness.\nThe other two chromatids code for brown eyes, red hair and normal vision.\n\nDuring crossing over one chromatid from each pair forming the tetrad literally have ends "crossing over" and bits of information are exchanged.\n\nAfter crossing over, you have 4 chromatids with unique codings.  Using the example above, we may now have:\n-blue eyes, brown hair, shortsightedness\n-blue eyes, red hair, normal vision\n-brown eyes, brown hair, shortsightedness\n-brown eyes, red hair, normal vision\n\nWe now have genetic variability in each gamete produced from this meiotic division.