I Proteus Vulgaris an acid fast stain or a non acid fast stain?
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No.  Proteus vulgaris is not one of the acid fast organisms.  It'll stain just fine with Gram's stain.\n(Gram Negative rod)
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If you are trained in microbiology you can tell by eye.  Plate out the bacteria (for single colonies) onto a universal media that is suitable for your target bacteria,  ie CLED, Maconkeys, Blood, Chocolate.  Incubate until there is a growth (usually 24hrs at 37 degreees celcsius is aerobic).  Look at the plate, are the colonies uniform in appearence.\n\nIf you are using liquid culture youu could use microscopy before plating out.  Either a gram stain or a direct (wet prep). This would let you know if you had all gram positives or all gram negatives or all rods or all  cocci or a mixture.  You would still have to plate out to make sure only one colony type grows.
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You can also perform classical staining according to Gram, B. subtilis is G+ rod, than you inoculate meat-extract agar plate, incubate it for several days and old culture you can check for endospores microscopically after staining according to Wirtz and Couhlin. Spores are typically located.\nSome experiences are needed to identificate Bacilli this way.
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The staining was a factor, but now with triple-antibiotic creams, they seem to work even better, without poisoning the skin/body\n\nPS Iodine, does a similar job, I think.
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Usually gram negative bacteria willl always stain Gram negative.  The property of the Gram Negative bacterai that makes them stain Gram negative is their thin cell wall (which is composed of peptidoglycan).  \n\nI believe what you intend to ask is under what conditions willl a Gram Positive bacteria turn into a Gram Negative bacteria.  \nThe reason that Gram Positive bacteria stain gram positive is becuase their thick peptidoglycan cell wall retains a purple stain in the gram staining process.  When Gram positive bacteria are grwon in a single culture, such as a test tube, for a long period of time without adding any additional nutrients to the broth then the broth will have a low nutrient concentration.  When there is a low amount of nutirents present then the Gram positive bacteria will only make a thin cell wall.  By making a thin cell wall the gram positive bacteria will not retain the purple stain and will stain gram negative.\n\nJust remember that the bacteria is still Gram positive but just composes a thin cell wall that makes it stain gram negative.  It is for this reason that it is suggested to only Gram stain a fresh bacterial culture as this will usually prevent a false gram negative stain
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lol, last minute lab write ups. afaik enzymes dont change colour, as they are not supposed to change so id bet there is something else that the enzyme works on. better put up the other reagents to get a better answer
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This article answers your question.\n\nhttp://www.hhmi.org/cgi-bin/askascientist/highlight.pl?kw=&file=answers%2Fgeneral%2Fans_043.html
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does this help?\nhttp://www.emedicine.com/med/byname/proteus-infections.htm
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Ah, AP biology class. Let me guess, pureed beef liver and hydrogen peroxide? I hated that lab, but I remember writing the lab report.\n\nYour results do not mean enzymes work harder if there is more substrate around. First, visualize the solution on a molecular level and picture a lone enzyme molecule floating in water with substrate. The probability of an enzyme molecule bumping into a substrate molecule and doing its thing in any given microsecond increases if there are more substrate molecules to bump into!\n\nDid that help?
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Bacteria are translucent so they must be stained to visualize with a light microscope. You can use numerous stains (crystal violet, methylene blue, safrinin) to color them so they can be seen.
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Please visit:\nhttp://www.homeofpoi.com/articles/colouredflames.php
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Rapid Plasma Reagin -- it's a test for syphilis.
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It is Gram positive (stains purple after a Gram stain), has endospores (can tell from a simple stain or from a Schaffer-Fulton endospore stain), and is rod-shaped.
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Gel Electrophoresis is a biggie\nYou can take advantage of their isoelectric points\nYou can take advantage of their acid/base chemistry \nYou can selectiviely stain them (ninhydrin comes to mind)
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You need to specify what kind of stains you are using. If you are using a Mito-Tracker stain, you would see the mitochondria. If you are staining them with FITC-Phalloidin, you can see the actin filaments. If you use DAPI, you will stain the nucleus. \n\nSo it depends on your stain.
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Any chemical that will react with oxygen can give a color change; it just depends on how fast a color change you want.  Generally, anything that reacts quickly will also be fairly dangerous to handle.  Most plastics also have antioxidents mixed in to limit their degradation in air, which will also limit your color change.\n\nThe chemical with the fastest reaction that comes to mind is phosphorus - which will ignite in oxygen.\n\nYou could also try iron or copper, which would be a much slower reaction, but also much safer.  You can mix a weak acid (like acetic acid - vinegar) in with the plastic to accelerate the reaction. \n\nBy the way - for this to work, you will need to prepare the plastic in a nitrogen (or other non-oxygen) atmosphere.
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Primary stain - Carbofuscin.\nDecolorizer - Acidified alcohol\nCounterstain - Methylene blue.\n\nAcid fast bacili retain the primary stain and are Red.\nNon-acid fast bacili stain blue.