How do you clone plants?
157058
Take clipping from original plant and plant the clipping.
802651
My God - seven prat answers - the question was HOW, not WHERE--------\n\nHowever - they are specially grown after years of scientific/botanic experimenting between the various types so that this seedless one is the result of many years' research. and are reproduced from root cutting as well as stem cuttings.
265671
Depends on what kind of cloning you are talking about.\n\nThe term "cloning" can be used to describe inserting a foreign gene into a bacteria... or creating a genetically identical copy of organism.\n\nTo create an identical copy... you would require an oocyte (an egg) and a somatic cell.  You'd need to remove all the DNA from the egg and the somatic cell... then inject the DNA into the egg.  Along with other factors and after growing... you will have an organism gentically identical to the orriginal.\n\nCloning genes into bacteria involves a plasmid (circular piece of non-chromosomal DNA).  You would need to open up the plasmid using restriction enzymes (DNA scissors basically) and cut the gene of interest out with the same enzyme.  Mix them then insert into your bacteria by your favorite method (electroporation, chemical method, heat/cold shock, micro-injection).  Let the bacteria grow (all bacteria in a colony are gentically identical to the parent... for the most part)
524082
If you change something it is not a clone anymore. It has to be an exact copy to be a clone. Your a bit obsessed with cloning aren't you?
466802
High-throughput DNA Isolation Protocol \n\nCTAB Method \n\n1.      Add 80 ul 2-mercaptoethanol to 40 ml CTAB buffer (see recipe below) right before use (0.2%) and mix. \n\n2.      Preheat CTAB DNA isolation buffer to 65°C in a water bath. \n\n3.      Grind freeze-dried leaf tissue (~100mg) in a Mixer Mill for 3-5 min at 30 time/sec with one 3mm steel bead in a 1.1 ml  tube strip from Qiagen\n\n4.      Carefully pour beads out of tubes and add 300 ul CTAB buffer into each tube with a multi-channel pipette (if a plate is used, you can leave beads in until the end of work)\n\n5.      Cap the tube strips and label each strip, caps and rack, incubate the samples in 65 °C water bath for 60-80 min, use shipping tape to secure the rack and lid to prevent caps from openning up while inverting the rack to mix samples.\n\n6.      Mix samples every 15 min by inverting rack 5-10 times to make sure plant tissue mixes well with the buffer. Watch for sample leaking from caps. \n\n7.      Take samples out of water bath, briefly spin sample box in a centrifuge for 1 min at 1500 rpm (balance box weight before spinning), separate caps from tube and put the cap strips on clean paper and arrange them in order so that they can be reused. \n\n8.      Add 300 Chl/IAA (25:1) into each tube with a multi-channel pipette, cap tube with original cap strips, put a plastic board between tube cap and rack lid to make sure every strip cap gets the same pressure from the lid, cover the box with lid, secure the lid with shipping tape. Mix the samples by inverting the box slowly for 15 min (Chloroform can create pressure and cause leaks of chloroform, so the tubes have to be tightly capped).\n\n9.      Centrifuge at 5,700 rpm  for 10 min. \n\n10.  Transfer the aqueous phase using a wide-bore pipette tip (about 200ul) into a labeled clean plate (650 ul plate), add 150 ul of cold isopropanol (-20 °C), securely cover the plate with a plate cover (or cap strips) and mix gently by inverting the plate several times to precipitate DNA. \n\n11.  Recover the DNA by centrifuging at 5,700 rpm (6100g) for 10 min. Discard the supernatant immediately by suddenly inverting the plate and dry it briefly on several layers of paper towels by briefly touching the plate on paper towels two to three times on different positions of towel to remove excess liquid. This step should be very quick (a few seconds). Then turn the plate back upright and air-dry samples to remove excess liquid for ~10 min.\n\n12.  Add 500 ul of 70% Ethanol directly to the pellet, cover the plate, and invert it gently several times to wash  for 5 min. \n\n13.  Centrifuge at 5,700 rpm for 8 min, and quickly discard wash buffer as described previously (check DNA pellet in each well to make sure DNA stayed in the bottoms of wells\n\n14.  Air-dry DNA pellet at room temperature for ~20 mins to remove excess liquid.\n\n15.  Re-suspend the DNA pellet in 200 u1 of ddH2O and leave it at 4°C overnight \n\n16.  DNA concentration will be ~100ng /ul and can be determined using either an agarose gel or a spectrophotometer. \n\n \n\n                                                                            CTAB  DNA Isolation Buffer (500 ml) \n\n \n\n2% (w/v) CTAB (sigma)\n 10.0 g\n \n1.4M NaCI\n 40.91 g\n \n20 mM EDTA\n 20 m1 of 0.5 M EDTA \n \n100 mM Tris. HCI (pH 8.0)\n 50 ml of 1.0 M\n \nAdd ddH2O to make a final volume of 500 ml \n \n\n \n\nSDS Method \n\n1.      1-2 young leaves (~100mg) are placed in a labeled 1.1-ml collection tube or in a plate well, freeze-dried, and ground for 3-5 min at 30x/sec to a fine powder using a Mixer Mill and one 3-4mm steel bead in each well. (To isolate DNA from dry seeds, half to 3 seeds are wrapped with a weighing paper, crushed with pliers, placed in a col1ection tube or   in a deep-well plate ).\n\n2.      Preheat extraction buffer to 65°C in a water bath. (If the tissue samples are stored at -20°C, allow them to warm up to room temperature).\n\n3.      Add 0.2% (v/v) ß-mercap
122360
design it just like any other plant, however this is realyl complicated.  I bet you 10 points nobody answers this correctly.
87726
The way it is treated will affect its life. Should you place the stem in a polystyrene cube, floating in a tank of aerated water, stocked with nutrients and maintained at 26 degrees celsius, you would begin generating a healthy "clone" of the original plant within three weeks.
565076
you could make antibiotic/antiviral resistant strains of viruses or bacterea. change the genetics of plants to create more of a certain toxin so you can get a greater yield of toxin per plant. The most efficient way to distribute the toxin would be through the water supply. many places either do not check their water or do not check it well enough.
648771
ro plants and spares ????? can u pls be spcific?
799301
hope you will get something useful in this link\nhttp://en.wikipedia.org/wiki/Human_cloning
55818
Human cloning is practically possible. That is ,it can be done. But the actual human cloning is at present; illegal.\nAt the same time animal cloning has not even been perfected. Only 1 or 2 offsprings are gotten from about 100 experiments.
1150426
Plant tissue cultures isolated from even a single cell can show variation after repeated subculture. Distinct lines can be selected with their own particular morphology and physiology. It suggests that the tissue culture contains a population of genotypes whose proportion can be altered by imposing an appropriate selection pressure. This variation can be transmitted to plants regenerated from the tissue cultures, and is called somaclonal variation. It provides an additional source of novel variation for exploitation by plant breeders.
225280
You likely won't find one on-line...your best bet would be to contact a plant and see if they will provide a copy of  their manual to you.
270273
I think you could only if you integrated the necessary necromantic rituals into the cloning process.  You could clone the tissue, but it would grow as a living creature.  You'd then have to euthanize it and then raise it from the dead.
996661
There are two primary ways to propagate roses. Asexual reproduction is usually used to produce a duplicate of the parent plant. Sexual reproduction, i.e. growing roses from seed, is primarily used to create new varieties of roses. \nCommon methods of asexual propagation of roses are softwood rooting, hardwood rooting, and bud grafting. Limited space permits only a brief description of softwood rooting. \n\nOld Roses, English Roses and Miniatures are generally good candidates for rooting cuttings because they usually grow vigorously on their own roots. Modern Roses such as Hybrid Teas and Floribundas are usually sold budded onto different rootstock. Some Modern Roses do grow vigorously on their own roots, while others do not. Below is a description of softwood rooting from Karen Baldwin with some changes. \n\nROSE PROPAGATION A LA ZIPLOCK BAGGIES \n\nMAKING THE CUTTING \n\nPreferably take a cutting on which the bloom is barely spent, so that all the petals have just recently dropped off. It is okay to take a cutting earlier, but at least make sure color is showing in the bud. These are indications of the maturity of the wood in the stem -- you want something in between the extremes of greenwood and hardwood. \nTry to have at least four separate leafsets under the bloom, and a five-leaflet set at the bottom of the cutting. (Each spot where the leafsets meet the stem forms a "node," where the bud eyes are, and from which roots can form. Hybrid teas tend to have fewer "nodes" spaced farther apart than Old World roses, and thus require a longer cutting, generally speaking). Make a clean bottom cut with a sharp, clean pruning tool 1" below the last node. Try to leave about 1/2" of cane above the top leafset. \nKeep your cuttings fresh in water while you gather more, until you're ready to plant them. \nPLANTING THE CUTTING: \n\nFill a 1-gallon zip lock baggie 1/4 to 1/3 full (about 3") with STERILE loose potting mix. (e.g., 1/2 peter's potting soil and 1/2 vermiculite). A 2-gallon ziplock baggie may be better since it will give the leaves more room, but use the same depth of soil you'd use in a 1-gallon baggie, since you'll be watching for roots growing through it, later. \nMoisten the mix but do not make it extremely wet. Use 1 tsp. miracle gro per 1 quart of water, to provide some initial nutrients (which may help avoid yellowing and leaf-drop). With your hands, firm the soil down well, within the baggie. The soil should be very damp, but there should be no standing water in the bottom. \nSnip off the stem a little above the top-most leaf set (i.e., remove the flowering part). Try to leave about 1/2" of cane above the top leafset. \nStrip off the bottom two sets of leaves (where the stem will be pushed into the soil). \nScore the bottom part of the stem along its length (vertically) for an inch or so. (An exacto-knife works nicely for this purpose, but fingernails will do fine.) Roots will form along this score. \nDip scored end of cutting into rooting compound, a couple inches deep. Knock off the excess (you can get too thick a layer). Stick the cutting a couple of inches into the soil. \nIf insects have eaten the leaves during previous rooting attempts, you may wish sprinkle a very small amount of diazinon or other insecticide on the soil surface. Be especially careful if you are using chemicals indoors. \nMist the cutting and the interior surfaces of the baggie with a spray bottle filled with the following mix (to avoid fungus and mildew growth in the closed "terrarium" environment). Do not use spraycan fungicides or insecticides ... in the closed environment, the chemicals can overwhelm then kill a new young plant. 1 quart water 1 tsp. miracle gro 1 tsp. baking soda (no more!) 2-3 drops dishwashing liquid (to make it cling) \nZip baggie almost shut. Breathe into it 'til it expands kinda like a balloon, and zip the rest of the way closed. (Keep it closed unles
908302
http://www.google.com/search?hl=en&sa=X&oi=spell&resnum=0&ct=result&cd=1&q=plant+propagation&spell=1\n\nhttp://forums.gardenweb.com/forums/load/propa/msg0212575212518.html?2\nhttp://www.sciencenetlinks.com/Lessons.cfm?DocID=22\nhttp://classroom.all-science-fair-projects.com/forum/showthread.php?t=1161
140134
some places store tissue which is capable of regrowing into the whole plant (e.g. meristem) in liquid nitrogen. They can get them regrowing after storage. \nBut every plant species (and even varieties) requires a different adapted protocol of how to best freeze them and thaw them. Usually freeze protectants (e.g. mannitol) are added before freezing them.