What is Enzymed-Link Immuno Sorbent Assay?
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ELISA(Enzyme Linked Immuno Sorbent Assay)- a type of blood test to detect the antibodies to a suspected infecting organism.
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www.austin.cc.tx.us/kotrla/immunohe.htm\nwww.irvingcrowley.com/cls/immuno.htm\nwww.lww.com/product/?0-397-55469-9
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Yes ther is .. just visit this http://labs.google.com/
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This depends on the test: What antigen are you testing for, how was it produced etc. Different ELISAs can have different sensitivity and specitivity.\nFor a better answer you have to give more details in your question.
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fluorescent microscopy will work for this.  First fix the cells (3% paraformaldehyde) then permeabilize (0.5% triton-x) and then stain with antibody to your protein of interest.
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There are several ways to do it.. \n\nYou can perform an ELISA (Enzyme linked immunosorbant assay) this technique involves the use of antibodies specific for the cytokine you wish to measure and then a secondary antibody to bind to the antibody against the cytokine which is tagged with an enzyme that can change colour when a certain chemical is added allowing the whole reaction to be quantified and measure the amount of cytokine being produced.\n\nAnother method to use is reverse transcriptase PCR - this measures the amount of mRNA made of the cytokine of interest.. However controversy exists over this method as it is not known if the amount of mRNA present is truly indicative of the amount of protein made.\n\nHope this helps..\n\nKatie
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Depends if you are doing flow cytometry testing, serology testing or DNA/PCR typing.  If you are doing serolgy testing, then usually you have pre-loaded serum panels on microplates that will be indicative of certain antigens.  You add the patients lymphocytes and incubate.  You then stain using a live dead stain, with fluorescent green indicating live cells (no cross reactivity) or red, indicating dead cells or a rejection reaction.  From these results you can decide which antige3ns the patient would potentially react (or not) with. That's the short answer as there are other lab protocols that encompass HLA testing.
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isoelectric point of hen egg white lysozyme: 10.7
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Iodoacetamide is an alkylating sulfhydryl reagent used for used for peptide mapping purposes. Its actions are similar to those of iodoacetate.
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The most popular sandwich assays are enzyme-linked immunosorbent assays (ELISAs), although newer assays, such as those using flow cytometry or based on amplification of the sample or its signal, are challenging their dominance.
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I believe you're asking about a monoclonal antibody. Perhaps when this was copied the "ocl" looked like a "d" and the "n" looked like an "r."\n\nhttp://www.bartleby.com/65/mo/monoclona.html
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Sounds like a typo.  Call the lab that performed the test.
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Hi - here are some ideas without seeing your actual data.  First - the system was too hot during transfer to  the membrane - be sure you aren't running it at too high  a voltage.   Second, not an issue with the  blot,  but with  the gel  - was it expired?  not fully polymerized? were the  wells free of debris when loading  samples?   sometimes with highly concentrated samples, especially cell lysates,  this  can  happen  because  of  viscosity  in t he  sample  (from  DNA for example). good  luck.
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Part of the cell mediated antigen-antibody defense system.
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High performance liguid chromatography (HPLC), very high density acrylamide gels, such as 16-20%, immunopurification using specific anitbodies are among the most common.
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Polyclonal\n\nPolyclonal antibodies are antibodies that are derived from several different t B-cell lines. The selections of antibody molecules bind to different parts of the antigen, with different binding affinities.  Within a polyclonal sample, some of the antibodies will be specific for the antigen with which the animal was immunised.  The rest of the antibodies have been obtained from meetings with other foreign antigens that the animal has been exposed to through its life time.\n\nThe production of polyclonal antibodies begins with the immunisation of an animal, usually a rabbit.  Once the rabbit has been immunised, it is restrained in a special cage and its ear is prepared for removing a blood sample from the animal.  A razor blade is used to cut the vein on the rabbit’s ear.  The blood is then collected in glass tubes so the blood can clot, as this will be more efficient and more serum can be obtained with minimal hemolysis.  When the blood clots a wooden applicator is used to rim the edges of the clot, this step is important, as it results in getting the maximum amount of serum from the blood.  The antibodies are then purified directly from the serum.\n\nThere are some advantages for using polyclonal antibodies, however there are also \ndisadvantages.  Some advantages of using polyclonal antibodies are:\n\n\nAdvantages\n\n* After as little as 2-3 months after the initial immunisation of the animal, multiple                   \n   subclass and high affinity polyclonal antibodies can be isolated.\n\n* Polyclonal antibodies are relatively inexpensive to produce.\n\n* From one immunised animal, large quantities of polyclonal antibodies can be          \n   produced. (up to 10mg/ml)\n\n* Polyclonal antibodies provide an overall look at an immune response, due to them  \n   containing an entire antigen specific population\n\n\nSome disadvantages from using polyclonal antibodies are:\n\n\nDisadvantages\n\n* To obtain the correct specificity, the antibodies have to be highly purified\n\n* The amount of antibodies that can be obtained are limited to the life span of the  \n   immunised animal \n\n* Polyclonal antisera recognises many antigenic determinants, making it difficult to \n   study individual parts of the complex antigens.\n\n* As a result of changes in antibody affinity, specificity, and subclass, different \n   bleedings have to be characterised individually\n\n\nMonoclonal\n\n\nUnlike polyclonal, producing monoclonal antibodies is a much more complex process.  Monoclonal antibodies are produce from one clone of cells rather than many different clones like polyclonal antibodies.  This results in the antibody molecules being exactly the same and they bind to the same antigenic site with identical binding affinities.  To produce monoclonal antibodies, clones of a single hybrid cell are formed in a laboratory.  This is achieved by fusing a B cell with a tumour cell.\n\nThe process involves repeatedly immunising an inbred mouse with an antigen.  After several immunisations the animal’s spleen is removed. The spleen is then removed as it is produces the B-cells. Due to the spleen cells not being able to be cultured, the cells are combined with myeloma cells. The combination results in the production hybridoma cells.  Hybridoma cells that produce antibodies that are not specific for the antigen are easily screened out with an ELISA, making it easier to find the antibodies specific to the antigen.  Once a selected, a single hybridoma line is injected into a healthy mouse.  The injection causes a tumour to grow inside the mouse.  This is due to hybridoma cells and myeloma cells being able to produce tumours.  As the tumour grows it produces a fluid which is full of monoclonal antibodies.  This fluid is known as ascites.  Monoclonal antibodies are produced due to the hybridoma cell being produced from a single B-cell.\n\nSome advantages of monoclonal antibodies are:\n\nAdvantages\n\n\n*   Antibodies with low–affinity b
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ChemDraw from CambridgeSoft Corporation may be help you. Look at www.cambridgesoft.com