What is chromatography?
116691
Chromatographyis a way to seperate out different parts of a mixture.  The idea is that you pass a fluid (either a gas or a liquid) down a long path, and the different molecules will move at different rates.  THat's the long and short of it.  So the molecules that move fastest get to the end first, and the slowest come out last.  You seperate things by making them race, and similar chemicals will take a similar amount of time to finish the race.\n\nI'm assuming that you're getting confused by things like "solid phase" and "mobile phase" and "bind affinity" and such.  The idea though is that you set up different types of obstacles depending on which things you want to win the race.  Sometimes this is dine by using something with a lot of positive charges, so that things with negative charges will get stuck and the things that are neutral or positively charged will win the race. Sometimes you sort things by size, by using a gel as an obstable with a bunch of little pores in it: little guys get lost inside and the fat guy wins the race.  With gases, you can just make the race really, really, really long, and the lightest parts will move the quickest while the hevy boys lag behind (this is a way that uranium is enriched for nuclear reactors and bombs).
1110554
I believe you mean "titration".\n\nTitration is a way to measure the concentration of a dissolved substance by seeing exactly how much of a neutralizing substance you have to add to the mixture to bring it to a neutral concentration.  \n\nThis is usually done by adding drops of a neutralizing substance of known strength until the original solution becomes neutral.  By calculating exactly how much neutralizer was needed, you know the strength of the original mixture.
433591
Gas-liquid chromatography is useful for a wide range of non-polaranalytes, but poor for thermally labile molecules. \nAtomic absorption spectroscopy reveals identity and concentration of atoms and ions,it does not provide any other information about molecules,bonds etc.
919511
Chromatography is used mainly to quantify or measure purity and the concentrations of impurities present in a sample.\n\nTLC is used to used in many Quality Laboratories to measure the impurities present in raw materials.  Most amino acids, along with some other active pharmaceutical ingredients, used in the pharmaceutical/biotechnology industry require impurity testing by TLC.
534433
Because chromatography just separates different kinds of molecules. It doesn't break apart the molecules themselves.
721022
Well, I do not know that syllabus.  You will need to sift useful answers.\n\nI would use:\n\ndifferent solubility in solvents\ndifferent boiling points\nrecrystallise one out of a mixed solution\n\nYou might be at too junior a level to know about ion exchange resins, and other chromatography techniques.
448305
That's where you scrape the TLC coating where your analyte of interest makes a spot.  Then you can dissolve the analyte using a suitable solvent and centrifuge to let the TLC solid stationary phase settle to the bottom.\n\nThis process allows you to separate an analyte of interest from other compounds it is mixed with.
947826
You could check theses sites:\n\nhttp://www.liv.ac.uk/Chemistry/Links/reforganic.html\n\nhttp://matematicas.udea.edu.co/~carlopez/index2a.html\n\nhttp://www.anachem.umu.se/cgi-bin/jumpstation.exe?Chromatography\n\nhttp://www.organicworldwide.net/
892793
Metabolism is the sum of all chemical reactions within the body.  the basics can be broken down into 2 groups.. Catabolism and anabolism.  Catabolism is the chemical processes that break down complex molecules to release energy.  Anabolism is the process to build up complex chemical compounds from simpler chemical compounds using stored energy.  \nIf your want to know more you should run a search on Krebs cycle, Glycolosis, lipid metabolism, and Protien Anabolism/Catabolism
888975
There is no such thing as "mass chromatography"\n\nThere is 2 other options that you can be refering to  LC/GC/MS systems that separate the elution and atomize the solvent as it comes off the column, and send it to the quadrapole in the MS for detection of mass.\n\nThere is also GPC (Gel Permeation) Chromatography which is perhaps more what you might be looking for.   This type of system is similar to HPLC, but is lower pressure (100 bar) and instead of separation by Polarity, this particular column will separate by molecular mass; with the larger molecules having the shortest elution time, and the smaller molecules having the longer elution time.  This works best if used with a Refractive index detector.
507988
Since your question refers to only the types of detectors used in HPLC, I assume that you understand how HPLC works so I will not go into that.  Each chemical refracts light differently.  As the various components elute from the column, a beam of light is passed through the eluted flow.  The components come out as bands of individual components.  Each band or component refracts the light and this refraction is measured by a detector.  The signal from the detector is amplified and sent to a recording device which records the refractive index of that component.  From the refractive Index, the component can be identified and by the strength of the signal, the amount can be determined.  \n\nThe UV/Visible detector simply uses the different wavelengths of light to determine the various components being eluted.  Each component absorbs light rays differently.  The wavelength Can be varied depending on the class of compounds being examined.  This allows for maximum sensitivity of the detector.  As each component is eluted, it passes through the detector.  The absorbence of light gives the identification of the component and the amount of light absorbed is directly related to its concentration.
673219
Flocculation is the term given to the process of getting small particles to clump or aggregate together to formlarger particles and then to settle to the bottom.  Flocculation is often used to purify mixtures.  A chemical flocculant is added to a suspension, the suspended material then flocculates and settles to the bottom where it can be removed by filtration.\nPaul
460267
A mono Q column is loaded with very small beads that seperate molecules, usually proteins, by anion exchange- im not sure what level of detail you want.
1161990
Pharmacokineticsis a branch of pharmacology dedicated to the study of the time course of substances and their relationship with an organism or system. In practice, this discipline is applied mainly to drug substances, though in principle it concerns itself with all manner of compounds residing within an organism or system, such as nutrients, metabolites, endogenous hormones, toxins, etc. So, in basic terms, while pharmacodynamics explores what a drug does to the body, pharmacokinetics explores what the body does to the drug.
677394
Here are some of the more common applications of these techniques:\n\nGas Chromatography - Flame Ionization Detector, also called GC-FID.  This method is used to analyze mixtures of substances which have an appreciable vapor pressure and are capable of burning.  GC-FID is often used to periodically check a reaction mixture to see how far a particular reaction has gone.  It is also used to check for purity of the final product and to check for undesirable impurities.  \n\nHPLC can also be used in a similar way to GC-FID except that it is better at analysis of non-volatile compounds.  HPLC is often used to analyze the final purity of a product, or to analyze the concentration of a pharaceutical product in a physiological sample (blood, plasma, urine, etc).  \n\nUV-vis is good at measuring the concentration of a UV-absorbing compound in a matrix which contains no other UV-absorbers.  Sometimes you can take your final product and dissolve in non-uv absorbing solvent and measure the UV-vis absorbance to determine concentration or assay.\n\nThe above are just some of the more common uses.  There are many more uses for these techniques in the Pharma industry.
234588
It is called biochemestry
856165
Ion-exchange chromatography, or the more general ion chromatography, is a process that allows the separation of ions and polar molecules based on the charge properties of the molecules. It can be used for almost any kind of charged molecule including large proteins, small nucleotides and amino acids, with the experimental solution to be separated collectively known as the analyte. It is often used as a first step in protein purification\n\n\n\n\nThe ion chromatography is used for analysis of aqueous samples in parts-per-million (ppm) quantities of common anions (such as fluoride, chloride, nitrite, nitrate, and sulfate and common cations like lithium, sodium, ammonium, and potassium) using conductivity detectors. The chromatography also has the capability to analyze aqueous samples for parts-per-billion (ppb) quantities of hydrazine, monomethylhydrazine (MMH), and unsymmetrical dimethylhydrazine (UDMH).\n\nIon chromatography is a form of liquid chromatography that uses ion-exchange resins to separate atomic or molecular ions based on their interaction with the resin. Its greatest utility is for analysis of anions for which there are no other rapid analytical methods. It is also commonly used for cations and biochemical species such as amino acids and proteins. Most ion-exchange separations are done with pumps and metal columns.