what is an antisense regulation?
109654
http://opbs.okstate.edu/~melcher/mg/MGW2/MG2443.html\nhttp://mendel.biol.mu.edu/faculty/munroe.html\nhttp://www.pubmedcentral.nih.gov/articlerender.fcgi?tool=pubmed&pubmedid=9841677\n\nit's a bit complicated to try to explain so i hope these sites help
498173
Yes I have worked with RNA before. The main difficulty with all of the procedures is making sure you have gotten rid of the RNAses. They are very tough and stable, so you have to make sure everything has RNAse inhibitors (DEPC). I dont know enought about guinea fowl to know if they use similar polyA tails that humans, etc do. If not, the oligo-dT resin wouldnt have much to bind to. I dont know what your goal is but you could consider a PCR strategy to amplify what you are looking for. If you are looking for some tissue expression, you could look into buying premade mRNA from a company, which we used successfully before. hope that helps!
176863
A multiple cloning site (MCS) is a section of DNA, usually 20+ restriction sites.  These restriction sites are different areas that a gene can be inserted into, and represent complimentary ends of DNA sequences that will adhere to the section of DNA to be inserted.\n\nRestriction sites can have blunt, or 'sticky' ends (like an overhang), and are produced using restriction enzymes that cut the DNA in a specific way.  The DNA to be inserted is then cut with the opposing sequence, so that a join can be formed.\n\nMCS are typically found within engineered plasmids (circular double stranded DNA molecules), where they allow sections of inserted DNA be expressed.\n\nAn example of this would be the production of Insulin for sufferers of diabetes.  The gene for insulin is inserted into a the MCS of a plasmid.  The plasmid is then transfected into a bacteria or yeast cell, where the DNA within the plasmid is read, and expressed, leading to the production of insulin.
589633
1)there are 5 to 9 nucleotides on 5' mRNA named Shine Delgarno which are the match for 16SrRNA on 3' in the small subunit of ribosome.so 16SRNA conduct ribosome to AUG by binding to shine....and helps to initiate the protein synthesis.\n\n2)there are special sites on 16SrRNA which have a direct reaction with antisense on tRNA\n\n3)it binds to IF3(initiation factor3)
902807
the mRNA and tRNA are actually not controlled. The only thing controlled is the formation of RNA. It is formed from a complementary strand of DNA. That mRNA which goes through nuclear pores to the Ribosome. When it reaches to ribosome it attaches with the ribosome. The mRNA has a specific in a group of three like: A-U-T. Each code refers to a unique tRNA which then brings an amino acid to form a polypeptide chain. At the end there is a code which is subscribed as a STOP Code by the nucleus. This codes are what coontroll the process of protien synthysis. But the formation of mRNA from a complementaery strand is controlled by nucleus And the movement of mRNA as well as tRNA. is also controlled by nucleus (to some extent)\nHope this Helps
733994
DNA gel electrophoresis will tell you nothing about gene expression.\n\nGene expression means RNA polymerase is transcribing the DNA into RNA and ribosomes are translating the RNA to proteins.\n\nFirst we'll look at the RNA aspect.  \n\nYou can measure gene expression by constructs known as reporter fusions.  In a reporter fusion, the promoter sequences for the genes of interest are ligated into plasmids containing reporter genes (genes encoding easily measurable products, such as green fluorescent protein or beta glucuronidase).  You can then measure the differences in production of the markers in differen conditions.\n\nAlso using RNA you can perform microarray analysis, if the genome has been sequenced and arrays are available.  This technique has either whole genome PCR products, or long oligos specific to every ORF in the genome spotted on a glass slide.  You purify RNA from your cells in normal culture, and in the conditions you're curious about testing.  This RNA is then treated with reverse transcriptase to generate cDNA, which is fluorescently labeled and hybridized to the array.  A computer reads the relative intensities of the fluorescent tags and tells you the ratio of gene expression (I've left a lot out :P).\n\nYou can also do quantitative, real time, reverse-transcriptase PCR.  I'm not practiced in this method, but in general terms it uses reverse transcriptase for the first step of a PCR with your RNA as a template.  Then for successive rounds of PCR it monitors the amount of dsDNA, and then a computer estimates starting levels of RNA.\n\nThe other aspect is proteins.  You can run proteins on an acrylamide gel.  The simple way is just to run 2 samples, cells in normal culture, and cells in the experimental setup.  You'll see some sort of different banding pattern.  You can get fancy and purify differentially expressed bands, and send them off for edmond degredation for identification. \n\nYou can also be a little more hardcore about it and run a 2D gel, which gives considerably more resolving power for separating your bands.
237514
http://www.zymoresearch.com/protocols/protein/autol-instructions-4.pdf
849938
Just like the opposite of start codons, which initiate the translation process, these are the ones that terminate the process; i.e. the ones that tell the ribosomes to stop translation.\n\nAnd why they are that way depends on the type of protein being produced, and in which organism.
1121385
The answer is A, translation.\n\n\nDuring translation, the messenger RNA copies information from DNA to produce proteins. The information gets read by  ribosomes which facilitate the production of proteins.
976032
Most of them involve genetic "splicing," which involves removing a particular set of genes and splicing in a different set.  The purpose is to eliminate a particular disease, or make the plant/animal resistant, or to give a particular characteristic.
1172443
Insertions is  adding one or more extra nucleotides into the DNA. They are usually caused by transposable elements, or errors during replication of repeating elements (e.g. AT repeats). Insertions in the coding region a gene may alter splicing of the mRNA, or cause a shift in the reading frame (frameshift), both of which can significantly alter the gene product. Insertions can be reverted by excision of the transposable element.
197934
mRNA is messenger RNA contain codon with 3 bases to carry message from DNA for what type of Amino acid needed.  It in transcription.\nthe translation is called gene expression.  it makes proteins by using RNAs and AA in the cytoplasm.
46886
One start codon is used to provide tight regulation of translation (RNA to protein).  It also serves to limit damage that is caused if there is a mutation in the DNA.  It is less likely that a mutation would occur that would induce a start codon if there is only one of them and not three of them.
735010
The latest is a paper that got published in PNAS on transcriptional activation based on dsRNA.  The process is named RNAa for activation in contrast to RNAi for RNA interference.  This process is different from siRNA mediated post-transcriptional gene expression.  \n\nSmall dsRNAs induce transcriptional activation in human cells by Hideki Enokida et al\npnas. 006;103;17337-17342; originally published online Nov 3, 2006; PNAS
781789
mRNA is the RNA that carries information during transcription and translation. It has codons, which match up with the anticodons on tRNA. tRNA is the RNA that bonds to amino acids and transfers them to ribosomes, and mRNA. The reason RNA is used is because the cytoplasm is too damaging to DNA, so RNA is kind of a scapegoat. Hopefully, this helps.
1137561
http://www.pnas.org/cgi/content/full/102/19/7014
621230
At too low a temp. an enzyme remains inactive.\n\nAt too high a temp. It is killed or ineffective or destroyed.