i want to make up 200ml of low melting point agarose in TBE buffer. i don't know how much agarose/liter it is
99069
what for? for a DNA gel to separate 1000 bp to 10000 bp I'd try a 0.7 % gel (i.e. 1.4g in 200 ml). and run it for 30-45 min (watch the bromophenol blue) this is about 500bp
963455
Copper C-145000\n\nCopper  Includes Ag + Te  \nPhosphorus  Other deoxidizers may be used  \nTellurium  0.4 - 0.7
676978
you can only dilute it w/v as it is a solid. Just take 10gr and dissolve it in about 50ml distilled water, then make up to 100ml when it has cooled.
1039142
On a lab bench in a non-pressurized (variable volume) vessel at standard atmospheric pressure:\n\n21.07 * (44 *0.48 + 80 + 100 * 1 + 540 + 41 * 0.48) = 16030 cal\n= about 16 Kcal\n\nMore precisely, since the isobaric specific heat of liquid water (cwp)  and steam (cvp) are not constant over temperature, the real answer is calculated by integrating cwp and cvp over the temperature change. This more accurate value is 16083 cal\n\nIn a fixed volume container (isochoric), you will need to supply more or less heat depending on the volume. A 30 liter container will require the same amount of heat as the isobaric case. Volumes down to about 17 liters will require more heat, and volumes over 30 liters will require less.
616680
your solutions looks strange to me, mixing DiNaEDTA with Tris-HCl may be difficult, usually EDTA needs very high pH for getting dissolved and and Tris-HCl solutions has pH around 6, so maybe your problem is pH. Sorry about amounts, can´t say if don´t have the molecular weights of the reagents you are using. Also check your NaCl, 2.5M is a bit more than saturated for NaCl at room temperature.\nAnd, just wondering, this doesn´t look like a lysis buffer... looks just like a buffer.
831122
Use the smallest mechanical pipette for the purpose, i.e., if you need to pipette 150 microliters, then use a 200 microliter pipette with tips to match. (I think these are correct scales; it has been awhile since I used the mechanical pipettes). I find a greater accuracy than trying to use a 1 ml pipette to aliquot 50 microliters of solution. I used a multiple mechanical pipette for ELISA plates; however, they often have one or more channel that does not fill properly on the first try. Therefore, I suggest, taking up, releasing and retaking the solution in the pipette until you visually verify the liquid levels in all tips are approximately equal. \n\nOn the whole, I have found that the retaking up of solution (take up aliquot, release, then refill the tip 1-3 times) makes for the most accurate takeup of the proper measured amount on the mechanical pipette.
1041907
There are always instructions on the bottle the powder comes in. Remember if you want the plates to solidify, check that the powder contains the agar (ie - look at the ingredients) or else look at the agar ratio (1.5%) and determine how much you need to add.
1180455
TFM %:79 Min \nMoisture %:11-14 Max \nFree Alkalinity % (as NaoH):0.05 Max \nGlycerine %:0.4 max \nSalt % (as Nacl):1.0 max \nFe Content:30 PPM Max \nAcid Value:208 Min \nIodine Value:38-44 \nTitre (Deg Cent):42 Min \nColour in Lovibond 5 1/4" cell:20Y+ 3R max \nFatty Acid Blend:80:20 (Palm Oil: Kernal) \n  thats all i got
83196
I've usually made up a concentrated stock of various buffers and dilute it to the concentration I need.  From a 1M Tris stock it's a 1/100 dilution to 10mM and from a 1M MgCLs it's a 1/1000 dilution.  I would decide how many milliters I wanted to make, measure out the cesium powder then pipette out the amount of each Tris (1/100 of the total solution vulume) and Mg (1/1000 of the volume) then bring to the final volume with distilled water.  This works fine if the concentrated solutions don't interact and cause precipitates or something else like being nearly a saturated solution.
1071665
I think it is 200ppm but i am not for sure, it depends on what you use it for...you should contact the manufacturer (ecolab, right?) to get proper test strips to be sure
983150
Well, if you're using agarose you're looking at DNA.  THe higher concentrations of agarose separate smaller pieces of DNA more completely.\n\nLower concentrations generally cause smaller pieces to run together but allow for differentiation of bigger pieces.\n\nI tend to run 1% gels in TAE routinely, as measured by the DNA ladder we use (bands at 10k/8k/6k/5k/4k/2/k/1.5k/1.k/ 900/800/700/600/500/400/300/200/100)  that resolves everything pretty well except for maybe the 100-300 range, which can run off the gel if you're not careful.\n\nHowever, if I'm looking for size differences in smaller pieces, I'll run a 2% TAE gel (can tell 150 from 200 clearly, for example).  If I'm looking for size differences in bigger pieces, 6kb vs 6.2kb, I'll run a 0.7% TAE gel.\n\nThe molecular cloning manual has a chart for agarose concentrations and size range separations.
774925
Boil ¼ cup of water. Add one teaspoon of gelatin and one teaspoon of\nsugar. Stir for one minute until everything is dissolved.\n2. Cool the solution for ten minutes.\n3. Fill a petri dish to a little below the top edge.\n4. Close the petri dish by putting on the cover immediately. Let the\ncovered petri dish stand for at least four hours. Don’t touch the\ngrowth medium or you’ll add your own germs!\nThis recipe will fill two, small petri dishes. If you and your class plan to fill\nmore petri dishes, make more germ food by increasing the amount of your\ningredients proportionally.\n*Adapted from Gross But True Germs by Luann Colombo. See details in bibliography. If you don’t\nhave the materials needed for the experiment, you can purchase Ms. Colombo’s book, which includes\nall the supplies needed to conduct the experiment.
739325
According to Sigma-Aldrich the solubility of MgCl2.6H2O is 1M at 20C (or 20.3030 g/100ml solution), so it shouldn't be possible to prepare 4.5 M MgCl2 in just water. If there are more solutes involved which might increase the solubility of MgCl2 try dissolving those first and then the MgCl2.\n\nYet I saw some papers using 4.5 or 2.5 M...\n\nI don't know why there is this discrepancy between sigma-aldrich and the solubility provided in the link in the above answer which would correspond to 8 M if you consider 100ml of solution and not water. In reality you would have a significant change in volume when you add so much salt and the solubility would be much less but I don't know exactly how much. If you consider 167g of MgCl2.6H2O then you are adding 88.8 g of water which means that the total volume is at least 189 ml and thus the maximum concentration is 4.3 M.\n\nWikipedia gives a different number; 54.2g/100ml at 20C, without specifying if it is for the anhydrous or hexahydrate... so it would be 5.6 or 2.6M respectively... \n\nWhat a mess !!!\n\nMaybe you should also post the question in biology, since someone who has been doing such purifications can clarify the issue.\n\nAnother idea would be to prepare a 100ml saturated solution according to the lowest reported solubility (that of sigma-aldrich) using a 250 ml volumetric cylinder, and then from a pre-weighed amount of MgCl2.6H2O keep adding powder slowly until it doesn't dissolve any more. Weigh the powder that is left in order to find how much you dissolved, measure the final volume and calculate the molarity. If you achieve higher than 4.5 M, dilute.\n\nOR\n\nprepare a 4 M if the amount of powder that you couldn't dissolve is very small.\n\nor \n\nprepare 450ml of 1M and evaporate to 100ml of solution while praying that there will be no precipitation...\nThat's very desperate but I am running out of ideas...\n\nI must say the whole story is very fishy (finding very different solubility values)\n\nI'd go mad too...
839641
depends on how long you run it, gel type and conc, voltage, buffer
955628
For the reaction:\n\nMgO + 2 HCl  -->  MgCl2 + H2O\n\nThe temperature should go up, not down.  Only the initial and final temperature are needed.\n\nTypical values for students are between 110 an 150 kJ/mol MgO.  This is typical, not the best that can be done.
222016
I am in a Microbiology college course. The closest I can come to your question from my text is about 50 NM (nanometer).
47527
it depends how many bands you are likely to get. If you only expect one protein (one band-eg a purified protein) i found that loading about 3-6 micrograms was enough for a 12% acrylamide gel (I have not tried agarose for proteins). It will increase with  the number of proteins you might have (ie more bands, you need to load more) and the staining or visualisation method you will use.