subject :-gene expression & cell models?
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Response: Do your own fricken' homework
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"spatial" implies space/location and "temporal" implies temporary. \n   Thus, spatial and temporal gene expression signifies the gene/s which expresses their product/s according to their location in the body parts/organ(s) and also the duration for which they are expressed. so, these gene/s won't be constantly expressed as the housekeeping genes (needed for normally regulating the body functions) are.\n These genes are generally expressed according to their need for the proper function to be maintain. Once the sufficient amount Of product is formed and the purpose is solved the gene/s get silenced (BY MECHANISM OF FEEDBACK INHIBITION OR FEEDBACK SUPRESSION)
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I cant really write a story!\nThe cell membrane and lysozymes guard the cell from the things which cause harm.  The DNA in the nucleus determines what type of cell it is could be equivelent to religion or rules in society.  RNA transcribe from DNA and make 1000's of proreins which do 10000's of different thing in the cell.  This is a bit like a production line in a factory.  The proteins are made in steps using different types of RNA.  The proteins go through postage and packaging in the endoplasmic reticulum.  This is a bit like a post office because they get marked for postage for different regions in the cell.  The mitochondria would have to be the powerstations!\nThe cell cytoskeletion is like the motorway and roads.  \n\nThe DNA could also be thought of as a library and the RNA thought of as the students.\n\nCant think of anything else!
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Transformation\nNucleotides\nBase
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DNA gel electrophoresis will tell you nothing about gene expression.\n\nGene expression means RNA polymerase is transcribing the DNA into RNA and ribosomes are translating the RNA to proteins.\n\nFirst we'll look at the RNA aspect.  \n\nYou can measure gene expression by constructs known as reporter fusions.  In a reporter fusion, the promoter sequences for the genes of interest are ligated into plasmids containing reporter genes (genes encoding easily measurable products, such as green fluorescent protein or beta glucuronidase).  You can then measure the differences in production of the markers in differen conditions.\n\nAlso using RNA you can perform microarray analysis, if the genome has been sequenced and arrays are available.  This technique has either whole genome PCR products, or long oligos specific to every ORF in the genome spotted on a glass slide.  You purify RNA from your cells in normal culture, and in the conditions you're curious about testing.  This RNA is then treated with reverse transcriptase to generate cDNA, which is fluorescently labeled and hybridized to the array.  A computer reads the relative intensities of the fluorescent tags and tells you the ratio of gene expression (I've left a lot out :P).\n\nYou can also do quantitative, real time, reverse-transcriptase PCR.  I'm not practiced in this method, but in general terms it uses reverse transcriptase for the first step of a PCR with your RNA as a template.  Then for successive rounds of PCR it monitors the amount of dsDNA, and then a computer estimates starting levels of RNA.\n\nThe other aspect is proteins.  You can run proteins on an acrylamide gel.  The simple way is just to run 2 samples, cells in normal culture, and cells in the experimental setup.  You'll see some sort of different banding pattern.  You can get fancy and purify differentially expressed bands, and send them off for edmond degredation for identification. \n\nYou can also be a little more hardcore about it and run a 2D gel, which gives considerably more resolving power for separating your bands.
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there are databases with which you can do homology searches. best to know what genes you are looking for though as genome wide homology may be difficult to search for\ntry the ensemble database:\nhttp://www.ensembl.org/index.html\notherwise a broad literature search might be better suited. odds are someone else has already done a similar comparison.\n\nfound this old review\n\nhttp://www.sciencedirect.com/science?_ob=MImg&_imagekey=B6VS0-45765F9-2J-1&_cdi=6248&_user=911953&_orig=search&_coverDate=06%2F30%2F1997&_qd=1&_sk=999929996&view=c&wchp=dGLbVtz-zSkWA&md5=03f894feac6d92afb553850237f34bcf&ie=/sdarticle.pdf\n\nmight be of some help. if the link doesnt work, can be found using a Pubmed search.
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check out this link \n\nhttp://en.wikipedia.org/wiki/Cell_biology#Notable_cell_biologists
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http://diwww.epfl.ch/lami/biovision/ \n\nread this hear
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http://www.dent.ucla.edu/pic/members/microbio/mdphome.html
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YOU WILL LOVE THIS SITE.\nIT IS INTERACTIVE, ANIMATED & SOOO FULL OF KNOWLEDGE.\nTRUST ME!\nhttp://www.mhhe.com/biosci/esp/2002_general/Esp/default.htm
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1) DNA\n\n2) Chromosomes\n\n3) Genes
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Hmm Many great websites on biology have you looked any up? Start there does your text book have any resources for online help? Try that!! Cell = Tissue = Organ?
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Here ya go!  Good luck!\n\nhttp://www.emc.maricopa.edu/faculty/farabee/biobk/BioBookPLANTANAT.html
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You're trying to say you have a synthetic chromosome. I would check all the experiments for contamination, and check the cells for endogenous expression before jumping to that conclusion. There are a lot of hurdles to leap, as mentioned in the other posts.
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Try this:\nhttp://en.wikipedia.org/wiki/Cell_(biology)\n\nhttp://health-pictures.com/images/Human-cell.jpg
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i dont think you'll be able to access this without logging in to my school, so i'll paste it below in case. try publications at your school as well, pubmed is always good, as ia google.\n\nTransgenics\nThe production of transgenic mice is a gain-of-function approach to manipulation of the mouse genome (Clarke, 2002; Nagy et al., 2003; Hedrich, 2004). Transgenic mice can be designed for numerous purposes including: (1) identification and characterization of specific regulatory sequences; (2) ubiquitous or tissue-specific overexpression of a transgene; and (3) cell lineage ablation achieved by coupling a tissue-specific promoter to a sequence encoding a toxin which kills cells that express it. Transgenic mice are typically generated by microinjection of the transgene construct into the male pronucleus of fertilized oocytes, which are then reimplanted into the oviducts of foster females. Expression of the transgene is dependent on its site of integration, with individual insertion sites typically containing multiple copies of the transgene. It is important to keep in mind that transgene copy number and expression levels of the transgene do not necessarily correlate. When chromosomal integration occurs in the fertilized oocyte, the developing animal will be fully transgenic, since all nucleated cells in the animal will contain the transgene; however, if integration occurs later, at a postzygotic stage, the animal will be a mosaic, with some cells containing the transgene and others lacking it. Founders are identified through analysis of tail biopsies, and copy number and transgene integrity are then evaluated. To establish a transgenic line, a founder is bred to mice of a defined genetic background and offspring are characterized for expression of the transgene. Generally at least two founders should be maintained for experiments. Due to the nature of random integration, no two transgenic lines will be identical. Details on the identification and analysis of founders and the establishment of transgenic lines can be found in UNIT 23.9.\n\nTransgenics carrying large segments flanking a gene of interest isolated as bacterial artificial chromosomes (BACs) have been used to minimize the abovementioned variation due to the effects of different integration sites (UNIT 23.1). The versatility of this strategy has been significantly increased by the development of homologous recombination–based strategies to mutagenize and manipulate the BAC constructs (UNITS 1.16 & 23.11) and the use of regulated transgenics (e.g., with tetracycline; UNIT 23.12). In the GENSAT project (http://www.ncbi.nlm.nih.gov/projects/gensat; Gong et al., 2003), for example, an atlas of gene expression in the mouse central nervous system is based on large numbers of BAC transgenics in which the protein-coding sequences of genes expressed in the brain are replaced by an EGFP reporter.
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try this\n\nhttp://en.wikipedia.org/wiki/Epigenetics