Can acetone be used as a disinfectant for cell culture instead of 70% ethanol?
101932
acetone might dissolve the plastic.  it can ruin a synthetic gun stock.
184227
Manganese dioxide (MnO2) dissolved in a suitable organic solvent, such as acetone or dichloromethane, are more purposeful in the oxidation of benzylic alcohols.  Primary and secondary alcohols may be oxidised with PCC or Jone's reagent.
247317
There may be some resistant mammalian cells, but generally 70% ethanol will kill-not because of dehydration, but because the solution will dissolve the lipids in the cell membrane, leaving only the protein scaffolding-thus the cell will completely lose it's ionic and chemical balance.
1128825
rubbing alcohol - isopropyl alcohol, 2-propanol\nCH3CHOHCH3
168821
We have been using yeast cells to make alcoholic beverages for a long time, even before we knew what they were. And now, as a result of scientific investigation, people are studying ways to try to make yeast produce other useful materials. For example, if we could modify yeast to produce polyethylene, we would not have to make it out of oil and the US would buy less oil. \n\nAs far as animal cells go, people are studying ways to grow skin cells in big sheets, so that skin grafts can be done on people who have suffered deep or widespread burns. That will be a big, important discovery if we can do it.
111661
there are different levels of purity of water and other solvents. the more pure the solvent is the more expensive.\nfor most introductory labs or where the results aren't critical then walmart quality distilled or deionized water is ok.\nfor other purposes you'd want to use the really good stuff.
982225
NOOOOOOOOOO\n\nFirst of all 5% means 5 g/100ml.\nThe molecular weights are different (60 for CH3COOH and 36.5 for HCl), so 5% HCl has actually approximately double concentration of molecules than 5% CH3COOH.\nAlso CH3COOH is a weak acid, so not all of it will dissociate.\nHCl is strong acid so all of it will dissociate.\n\nThis means that you will end up with a solution that is way much more acidic (approximately 2000 times more acidic if I remember the Ka of acetic acid correctly)\n\nThen you have the problem of taste and smell, which will be different and the danger of having impurities in the HCl which are toxic.\n\nAre these reasons enough?
816577
I generally follow the recommendations provided by our safety officer at my academic lab.\n\nIf your liquid cultures can afford the extra volume, you can just add a lot of deionized h2o, that should stop the growing (by lysing the cells).  Then toss in the bio-hazard bag, for the autoclave.  \n\nOr, if you have access to an autoclave, then autoclave the culture, and you can probably dump down the drain.\n\nCheck with your safety officer!!\n\nIt was fun thinking about your question.  Pass on the good Karma!
498348
LIQUID-VAPOR EQUILIBRIUM IN BINARY SYSTEMS\nApparatus\nBoiling apparatus with heating mantle and variac (S), 1/100o thermometer (S),\nrefractometer (L), glass beads or boiling chips (S), weighing bottles (D), small\nstoppered vials (D), 250 ml n-hexane (S) and 250 ml absolute ethanol (S).\nOther binary systems which may be investigated are (1) cyclohexane (S)-\nethanol (S); (2) ethyl acetate (S)-ethanol (S); (3) Methanol (S)-ethyl acetate (S);\n(4) isopropanol (S)-n-hexane (S); (5) isopropanol (S)-cyclohexane (S); (6)\nisopropanol (S)-ethylacetate (S); (7) Acetone (S)-cyclohexane (S); (8) Acetone\n(S)- hexane (S); and (9) cyclohexane (S)-2-propanol (S), (10) 1 Propanol-water.\nCONSULT YOUR LAB INSTRUCTOR TO FIND OUT WHICH SYSTEM YOU\nARE TO INVESTIGATE.\nCAUTION: The ORGANIC LIQUIDS are TOXIC and FLAMMABLE! Use all\nnecessary precautions to avoid accidents!\nCAUTION: DISPOSE OF THESE ORGANIC LIQUIDS by placing in the\nWaste Containers provided. DO NOT POUR DOWN THE DRAIN!\n\nAssemble the apparatus with care; consult instructor if you are at\nall doubtful how to put the pieces together. A 3-way stopcock (shown in Figure\n2) is provided as a means of withdrawing samples of distillate and residue at the\nappropriate time. Best procedure is to withdraw distillate first, then the residue.\n\nProcedure\nClean the apparatus by rinsing with a few milliliters of pure n-hexane. Make sure\nvials are clean and DRY and have labels for marking. Add glass beads to flask\n(carefully).\nThere are three particularly important requirements in this experiment.\n• Make sure that the condensed distillate and the liquid residue samples are\ncollected under equilibrium conditions\n• Read the equilibrium temperature accurately\n• Analyze the compositions accurately\nPlace the thermometer in a position near the top and center of the three legs of\nthe first insert as seen in Figure 3. The hole in the second insert should be\nplaced pointed towards the condenser\nPlace 50 ml n-hexane in a flask. Adjust the variac (not more than 100 -110 volts)\nso that liquid boils "reversibly" (very moderate boiling with only very tiny bubbles\nformed) and so that distillate comes over at a rate which will fill the sample tube\nonce every two or three minutes.\nDraw off the first fraction (containing impurities) which comes over and discard\n(use waste container). When a constant boiling mixture is obtained, record the\nboiling point and withdraw samples from distillate and residue into marked vials.\nAdd 1 ml ethanol to the distillate in the flask, obtain equilibrium-boiling, record the\nboiling point, and again withdraw samples of distillate and residue for analysis.\nBoiling points and refractive indices of the distillate and residue are then\ndetermined after successive additions of 0.2, 0.5, 1, 2, 5 and 5 ml of ethanol. The\nrefractive indices are used to obtain the mole fractions of ethanol in these\nsolutions.\nClean the apparatus by rinsing with a few milliliters of ethanol, and repeat the\nprocess starting with 50 ml of ethanol in the flask. Now, however, boiling points\nare to be determined after successive additions of 0.5, 1, 2, 5, 15 and 20 ml of nhexane\nto the flask.\nIn order to construct a plot of refractive index versus mole percent ethanol, the\nrefractive indices are determined for the pure hexane and ethanol and for a\nseries of solutions containing accurately known weights of hexane and ethanol.\nMixtures about 5 ml in volume containing approximately 1 volume of ethanol to 1,\n3, and 6 volumes of hexane are convenient. To obtain a decent standardization\ncurve of refractive index versus mole fraction, a series of 6 to 8 solutions\ncontaining accurately known weights of n-hexane and ethanol must be prepared\nand their refractive indices measured. Only a few milliliters of each of these\nsolutions are required, so do not waste chemicals when preparing them. The\nrequired data for the vapor pressures of the organic liquids are availab
131640
I haven’t done any serious histology for over 10 years but…\nFirst off what do you mean by “without altering the mycelial growth”? Are you attempting to produce a live culture slide so that you can exmaine growth? If so then for God's sake don't use ethanol. Alcohol will certainly alter the mycelial growth. It will kill any growing mycelia. Alcohol also has a string tendency to cause shrinkage in soft tissues.\n\nDo you perhaps mean “without altering the mycelial structure”? That's a whole different story.\n\nLactophenol cotton blue is a combined stain and mounting fluid. The stain component (cotton blue) is designed to make the various cellular components contrast more so you can see them more easily. The lactophenol is just there as a mounting medium, not as a fixative. It serves approximately the same purpose as water on a wet slide but t lasts a little longer. Alcohol is a fixative. You can’t use it to replace the stain and it evaporates too fast to serve as a mounting medium. \n\nWhat do you want to use this slide for? How long do you need to store it?  I seem to recall, and a quick websearch seems to confirm, that lactic acid and acid fuschin does pretty much the same job as LCB. I’ll include one of the websites I turned up that suggest this. It also has a few other suggestions for mounting media/dye combinations that might suit you.\n\nSorry I can’t be more help. It’s probably because it’s been so long since I’ve done this, but I really don’t quite understand what you are trying to achieve.
288821
You know all the dangers? You sure? Acetone peroxide, known as "Mother of Satan" is extremely heat, friction, and shock sensitive, causing a huge explosion. In other words, heat, friction, and even a sharp motion could cause it to explode. If you have to ask Yahoo Answers how to dispose of acetone peroxide, you really shouldn't be near it. At all.\n\nEDIT: But if you really want to know, it is polar.
104161
You could rig a "Dean-Stark Trap" on the side of your condensor. That will eliminate water from your esterification (assuming its similar to the Fisher esterification) and force the production of more water and product, until one of the reagents is used up or an equilibrium is achieved.\n\nAnother method would be TLC, comparing the starting reaction mixture to the final product.\n\nSpectroscopy (NMR, IR) could be used.\n\nFinally, you could just put some of your sample into a GC-MS.
132557
Acetone is a polar molecule (i.e. the centers of positive and negative charges in its molecule do not exactly coincide). It infact is more polar than the water molecule (2.91 D > 1.85 D). Due to this (and of course other reasons I donot know) acetone has a very good solvent action and finds use in industry primarily as a solvent - including its use as a nail polish (which contains nitrocellulose; acetone probably breaks this down) remover.
570617
KMnO4 is an oxidizing agent.\n\nit will turn ethanol into ethanal (or acetylaldehyde).  if there is a great excess of potassium permanganate, it will turn ethanal into ethanoic acid (or acetic acid).\n\nbasically, both steps involve decreasing the number of bonds from carbon to hydrogen and increasing the number of bonds from carbon to oxygen.
152182
None that I know of. Anaerobic bacteria do not need oxygen to survive, and usually die in the presence of oxygen.\n\nTo survive, anaerobic bacteria use two types of repiration to survive: Fermentation and anaerobic repiration, both of which utilize glucose. \n\nThe article does mention that there are times the conversion of pyruvate to acetaldehyde generates CO2 and the conversion of acetaldehyde to ethanol regenerates NAD+. It doesn't mention which bacterium can facilitate said process.
160219
If you are from the philippines, chances are it's methanol. It's cheap and convenient to use (in terms of burning and handling in lab classes). Just be careful. Avoid inhalation, ingestion, and skin contact. It is known to be highly toxic.
661934
http://www.ask.com/web?q=why+is+it+important+to+avoid+contact+of+KMnO4+and+ethanol%3F+&qsrc=0&o=0