why did the culture using blood agar resulted in the highest number of growth compared to regular agar?
117048
depends what you mean by regular agar.  agar by itself has very little nutrition value.  when we make blood agar it is defibrinated blood added to blood agar base which has some nutrients (sodium chloride, tryptone, yeast extract and meat infusion).  the blood cells added to the blood agar base make it richer media as well as let you see what kind of hemolysis is happening to the blood cells.
668397
Depends if you want to grow pure cultures of microbes or not. If you don't have to have pure cultures, just put your leftovers in the fridge and forget'em. ;-)\n\nIf you want to grow pure cultures, you will need to know aseptic technique and what each type of microbe likes to eat. \n\nSome will go on a defined medium. That is one that you know exactly what it in there. Some have to have something complicated that we don't know exactly what all the components are, like yeast extract, beef heart infusion, that sort of thing.\n\nThen you need to know the temperature and humidity requirements of your bug and any special atmosphere conditions it need, like low oxygen or no oxygen.\n\nIt also helps to know how to store samples of your bug for future use. Can you freeze it? or do you have to lypholyse it?\n\nHope this helps.
1119929
I am ashamed to say I do not think of it so deeply, but you bring up wonderful points.  It surely is an amazing thing, the human body and the way it works.  I love not only the question but you left us with specifics so we did not just have to uhm blood good... I now have a little more knowledge to add to my trivial pursuit portfolio!!  hehehehe
298026
Surface area is an important factor in limiting cell growth because materials cannot enter the cell if it is too large.\n\nGo to the following website: http://staff.hartdistrict.org/mpearce/biology/chap3pt.htm\n\nTry to answer all the questions and then click on Check your Answers to get the solutions for all questions. You can do the same for each chapter.
762362
Selective media is used to select for the growth of a particular type of organism. They usually have a specific pH or component that inhibits the growth of certain organisms. MacConkey agar contains bile salts and crystal violet which inhibits the growth of Gram positive organisms. Eosin Methylene Blue agar (EMB) is also selective for Gram negatives. Phenylethlene agar (PEA) is selective for Gram positives and so is Mannitol Salt Agar (MSA). Hope that helps.
784116
When I was in Microbiology class, I was taught to stab the agar after streaking, to form sort of an Anaerobic chamber to look for Anaerobic hemolysis/growth. To this very day if and when I am working in microbiology, I still do a 4 way streak, and stab at the end. \nWhen streaking a BAP confirmation plate for tests such as a Quick Strep test, that stab can be of definite help.
983150
Well, if you're using agarose you're looking at DNA.  THe higher concentrations of agarose separate smaller pieces of DNA more completely.\n\nLower concentrations generally cause smaller pieces to run together but allow for differentiation of bigger pieces.\n\nI tend to run 1% gels in TAE routinely, as measured by the DNA ladder we use (bands at 10k/8k/6k/5k/4k/2/k/1.5k/1.k/ 900/800/700/600/500/400/300/200/100)  that resolves everything pretty well except for maybe the 100-300 range, which can run off the gel if you're not careful.\n\nHowever, if I'm looking for size differences in smaller pieces, I'll run a 2% TAE gel (can tell 150 from 200 clearly, for example).  If I'm looking for size differences in bigger pieces, 6kb vs 6.2kb, I'll run a 0.7% TAE gel.\n\nThe molecular cloning manual has a chart for agarose concentrations and size range separations.
258097
Is a simple culture enough?\nthe anerobic won't grow in air the aerobic will grow and give off O2. Yeasts will give off CO2.\nYou may need an autoclave to maintain temperature.\n{sorry not to be more helpful}
406962
When something grows exponentially it describes the relationship between two values.  One value grows at an exponenet (x2 or x3 or x3, ...) while the other item grows linearly (a+a+a+a)\n\nA simple example is a bacterial culture.  One value is time and it is the linear value.  The number of bacteri is the exponential vlaue (x2).\n\nSo one cell in the culture divides, giving us 2 cells.  Then those 2 cells each divide giving us 4 cells (from 2 to 4) then 4 double to 8, 8 to 16, 16 to 32.  Now look at the number of cells.\n\n1\n2\n4 = 2 squared (2x2)\n8 = 2 cubed (2x2x2)\n16 = 2 to the (4th power) [2x2x2x2]\n32 = 2 to the (5th power) [2x2x2x2x2]\n\n\nExponential decay is basically just the opposite.\n\nA radio signal degrades by a factor of 2 for every unit distance traveled.  The half-life of a radioactive substance is described by exponential decay.
496973
I knew a guy who got the highest marks I have ever seen, and his secret was that while he was reading something (relating to your work oviously) he would jot down points that he thought was pretty interesting or relative, all in his own words. When you write something down it sticks in your memory, and when its in your own words it tends to make more sense. Then, he would keep these pages and when it came time to study for a test or whatever, he would go over them and they made alot more sense than his text book! It really is the easier, most effective way of finding and retaining information.\nI tried it and shocked myself at how high my marks were, I still can remember certain points very vividly just from writing points in my own words.\n\nExample :\n\nFrom your text book : \n\nStudying bacteria. To establish that a certain bacterium causes a particular disease, bacteriologists follow four basic steps. These steps are called Koch's postulates, after the German physician Robert Koch, who formulated them in the late 1800's. The steps are: (1) Pathogenic (disease-causing) bacteria are taken from diseased animals. (2) The pathogenic bacteria are isolated and grown in a laboratory. (3) The laboratory-grown bacteria are injected into experimental animals. (4) Bacteria are isolated from the diseased experimental animals and shown to be the same kind as the original bacteria.\n\n\nMy own words :\n\nThere are 4 steps to finding out whether bacteria can cause certain diseases. Its called Koch's Postulates. Named after Robert Koch.\nHe thought this up in the late 1800's (remeber this date!).\n\n1. Take the pathogenic (means disease causing remember) bacteria from a sick animal.\n2.  Isolate the bacteria and grow it (In a lab)\n3.  Inject this grown bacteria into experimental animals.\n4.  The bacteria from those animals should be the same as the first lot you isolated from the first batch of animals.\n\nJust an example. See how much shorter and to the point it is? I just took the important parts. Make sure your facts are always accurate, as they end up replacing your text book, and make sure they make sense to you! Use plain English. Keep the notes in a safe place. Highlight dates with a bright highlighter.\nAnother thing that this guy used to do - when he finished an exam or test, he went over it like a forensic detective! He analysed it and double checked it! This is where you find and get rid of stupid mistakes. Good luck! You'll be fine!
725792
This is a guess, especially because I don't know what the diluent is made of, but maybe its because the viral coat is weaker and more susceptible to bursting without some sort of albumin present?\n\nGood luck.
709465
You should really ask your doctor a question like this, not a bunch of online people who are answering things because they are bored.\nBut, i'll give you a pointer anyway:\nAccording to Grey's Anatomy, it will not cause the cell to grow.\nBut, if you're trusting Grey's Anatomy for medical advice...well....i think your cell is the least of your worries.\nGood Luck.\nI sound rude, but thats just how i express concern.
107806
yes if the bacteria in the solution can break down and use that type of sugar. having more food means they will reproduce faster and will ferment more. although this can be dependant on if o2 is present or not. but in general as your question is stated...yes.
273694
The trick is deceptively simple.  A microbe is chosen--often a very fussy strain of Saccharomyces or Escherichia coli--and the DNA that encodes human fibrinogen is introduced into its genome.  This engenders the production of fibrinogen by the microbe; that fibrinogen can then be harvested and purified.\n\nThe same technology is used to produce human insulin, human growth hormone, and a wide array of other polypeptides useful to human medicine.\n\nWhat I find fascinating about it all is that the vat fermentation production system used to produce all this is a spinoff of the vat fermentation system used in the first mass production of penicillin, developed at the USDA's Agricultural Research Lab in Peoria, IL (Now known as the NCAUR) around the time of WWII...
536246
Because of bacteria and germs growing in it.
131640
I haven’t done any serious histology for over 10 years but…\nFirst off what do you mean by “without altering the mycelial growth”? Are you attempting to produce a live culture slide so that you can exmaine growth? If so then for God's sake don't use ethanol. Alcohol will certainly alter the mycelial growth. It will kill any growing mycelia. Alcohol also has a string tendency to cause shrinkage in soft tissues.\n\nDo you perhaps mean “without altering the mycelial structure”? That's a whole different story.\n\nLactophenol cotton blue is a combined stain and mounting fluid. The stain component (cotton blue) is designed to make the various cellular components contrast more so you can see them more easily. The lactophenol is just there as a mounting medium, not as a fixative. It serves approximately the same purpose as water on a wet slide but t lasts a little longer. Alcohol is a fixative. You can’t use it to replace the stain and it evaporates too fast to serve as a mounting medium. \n\nWhat do you want to use this slide for? How long do you need to store it?  I seem to recall, and a quick websearch seems to confirm, that lactic acid and acid fuschin does pretty much the same job as LCB. I’ll include one of the websites I turned up that suggest this. It also has a few other suggestions for mounting media/dye combinations that might suit you.\n\nSorry I can’t be more help. It’s probably because it’s been so long since I’ve done this, but I really don’t quite understand what you are trying to achieve.
476381
Actually, your teacher is wrong.  It all depends on the growth phase of the bacterial culture.   There are 3 phases in the growth of a bacterial culture; the lag phase, the log phase, and the stationary phase.  The lag phase is exactly what it describes...the bacteria divide veeeeerrrryyy slowly.  In simple terms, this is mainly due to low amounts of certain "growth signals".  It usually takes bacteria several hours to get out of lag phase and into log phase.  \n\nLog phase is the stage where the bacteria are dividing at a constant rate.  This is when you're going to take measurements for the growth rate.  Say, for example, that your cells have a doubling time of 1hr.  So if your beginning OD is 0.8 your end OD after an hour would be 1.6.  In this case B is twice b.  However using the same example, if we grow for 2 hours instead of 1, you've gone thorugh 2 doubling times and the final OD would be 3.2 verses 0.8 (B is 4x b).  So the relation between b and B is strictly dependant on the time (which is why "t" is a component of the equation) and growth rate of the cells.\n\nThe final phase of bacterial growth is the stationary phase.  This is where the number of bacteria increases to such a high density that further division is inhibited (by physical limitations such as nutrients, pH, cell/cell inhibition, etc) and the growth substatially slows.\n\nI hope this clearly answers your question.