can anyone help me with the pro and cons of using parrafin, plastic(GMA) or gelatin in the embedding process in histology?\n\nplease i hope only answers that are related to my question is posted. thanks in advance
136580
Parrafin is the most common; Cheap, easy to handle and non-toxic. Used on routine tissue where high resolution isn't required. H&E routine staining readily viable on paeeafin embeddied tissue. Plastic is used for non-routine tissue where high resolution is required. Also for specific histochemical staining techniques. Never used gelatin, sorry.
206318
Here is the one I recently made:\n\nTake a transparency sheet and take ear buds (used for over head projectors)- lots of them. Dip the top of cotton part, of a bunch of ear buds, in glue and place them vertically on the transparent sheet. Go on doing this to make a square tightly filled with ear buds.  Note: you will have to give support to the ear buds until they are glued completely. \n\nNow you can apply the glue on the other side of ear buds and place another transparent sheet. Paint the central region of the ear bud-sticks with black colour. If required, cover the sides. It now looks like a square piece taken from a region in the plasma membrane.\n\nThe cotton ends now look like the hydrophobic layers on either end while the central region looks like the hydrophilic ends of phospholipids. If you want to elaborate, you can include proteins in the bilayer! You can use colour papers, ribbons etc (you can stiffen them by dipping in some glue and drying in the shape you want; I have also seen hair-bands available that form specific ion-channel like structures. Goodluck!
595685
Poor thing. Hopefully the chemist is open by now. For the skin I used Aloe Vera Bio Active Gel on my kids, as well as calamine. Cool baths helped. Piriton for the itch. Not sure about the mouth, but you could try ice pops. And lots of calpol/nurofen of course. Hope he's over the worst soon. Hang on in there.
401873
hahaha! So you'd rather play with words such as \nsquamous cells,\northopharyngeal\nnasogastric\nrheology\npolyethyleneglycol\nglycogenolysis\nglyconeogenesis...\n\ngood for you!!
410062
I had a similar assignment when I was in school. I used food items on cardboard. Spaghetti - endoplasmic reticulum, peppercorns, ribosomes, pepperoni - nucleus, olive - nucleolus, hot dog - mitochondria, and so on. I put them all con cardboard. keep it simple, be creative, and have fun!
388307
Google L690.......All you can find is that it is a chemotherapy drug.  Haven't seen any images.
110471
http://www-medlib.med.utah.edu/kw/derm/pages/ac04_8.htm
468835
Sorry. I have prostate cancer also. About 18 months ago I had an biopsy and out of 12 biops one had a cancer cell. I have to go back in December to have another to see what stage that I am in. Before my Gleason score was 3+3. That is borderline for the stage that will spread. i bought a book from Mayo Clinic, and which I had not. There are so many options that it is unreal. I know one thing. I will not have a radical procedure done. Five days, and maybe more plus wearing a catheir bag for eight weeks. Serious bleeding, with that procedure, and also with the Labascopy also. If the cancer has spread into the lymp nodes, the radical procedure is the only way they can remove them. If that happens with me "I am just going to stick my head between my legs and kiss my ass good-bye". I will know my latest stage on December 5th..Wish me luck...
247266
log on to\n\nhttp://technology.infomine.com/biometmine/biopapers/biomet_advance.pdf\n\n:)
633318
We have a material that was designed to be used for "jungle type settings" its sticky, hardens quickly, works in a moist environement, and releases flouride.  unfortunately the designers and chemists made it too good and the general dentist started using it, so much so, that they increased the price  20 fold.  to make up for the losses from the drop in sales of their standard filling material.   Sad isn't it.\n\nIt's called Fuji IX and its expensive.  Since it is so cheap to make and produce,  you may consider calling the manufactuer, tell them about your project, they may well donate some to you.  But if you are going to a place without services, Definitely fuji IX is what you'd want to work with.
349893
i read the other posts and its strange but they totally disregard the facts !  nasal stem cells are compatible with all organs and usages, that what the newest paper released in the stem cell research fields says anyway, and the leading scientist said that embryonic was  not the only way that in the field it was the most convenient.  several of the ppl who responded just totally blew off the facts and said the nasal stem cells were for only a limited use and others supported the embryonic only because thats what they think works...neither view base on the facts. personally i think some ppl just don't want to know the truth. stem cell research is changing daily and for the person who said there is no funding they certainly don't know anything about it because millions was given last year alone in govt. grants, private companies and personal donations to this research. for once i'd like to see someone tell the truth with out bending it to fit their agenda. thanks for just pointing out the facts without being biased, i wish more ppl would do that.
419704
Hi Matthew!\n\nYou emailed me before, and I was just about to respond to your email (I never took it out of my in-box!  I didn't forget you!), but I just logged on to Yahoo! Answers (after a few months of being away) and saw your question here! So, I thought I would answer it here so others could see it too!\n\nYes, it hurts / "burns" a little, but it is so quick, it's like ripping off a band-aid.  AND YOU WILL FEEL SO MUCH BETTER AFTER THEY ARE OUT, that you will forget the pain within a minute.  \n\nMy drains were about 5 to 6 inches long....they were plastic....and they kinda looked like a plastic straw with a little "fanned shape" thing on the end.  I think there were holes both on the straw part and the fan part.  What makes them so uncomfortable is the fact that you basically have an inflexible piece of plastic sticking across your abs!\n\nDo not worry about the drains being removed....it is quick.  Good luck with your surgery....I hope your experience is as good as mine was!!!
549001
Hi -\n\nI'm sorry to hear about your brothers GBM.  \n\nCarboplatin has a half-life of 1-2 hours initially, and can be effective for up to 10 days.\nStarting dose is calculated based upon a persons weight, height, and kidney functions.  A typical dose for subjects with no previous treatment is 400mg/m2, at one time.  Dosing may be increased, depending on the severity of the disease, and the patients reaction to treatment.\nAs with most chemotherapy, this drug can suppress your immune system (bone marrow production of white blood cells).  Also, the patient can become thrombocytopenic, and anemic, which is usually temporary.\nIf your brother has a blood test, he might have an increased BUN, and serum creatinine, and liver enzymes may become elevated.  Once treatment ends, all of these symptoms usually cease.\nAs I am sure he knows, he can expect to experience GI upset and vomiting for the first 24 hours after dosing administration.\nCarboplatin is excreted primarily in the urine with 65% of the dose excreted after 12 hours and approximately 70% within 24 hours. \nAll of this information is available on the drug label, or the FDA website.\nHas his doctor mentioned temozolomide?\n\nGood Luck
228224
Yes I have worked with RNA before. The main difficulty with all of the procedures is making sure you have gotten rid of the RNAses. They are very tough and stable, so you have to make sure everything has RNAse inhibitors (DEPC). I dont know enought about guinea fowl to know if they use similar polyA tails that humans, etc do. If not, the oligo-dT resin wouldnt have much to bind to. I dont know what your goal is but you could consider a PCR strategy to amplify what you are looking for. If you are looking for some tissue expression, you could look into buying premade mRNA from a company, which we used successfully before. hope that helps!
212085
I would embed it in a liquid plastic resin that hardens clear. Most zoological lab suppliers would have this or know where to get it.
5515
Take a look at the websites below for more information. I can decode some of that for you. I can't tell you how serious it is...you need to find a doctor who will talk to you and explain things. \n\nLeiomyosarcoma is a rare cancer that affects smooth muscles. Low to intermediate grade is not as bad as advanced, so that part sounds positive. Focal necrosis means there is a part of the tissue sample that shows cells that are dying. I can't tell you what that means in the overall picture. The mitotic count is a measure of how many cells in the sample are dividing. HPF means high power field. I'm taking this to mean that when they looked under a high power microscope, 2-3 out of 10 cells were dividing. I can't give you a reference number for what is normal, but in general, more cells dividing would indicate the tumor is growing faster than a low number. \n\nImmunohistochemistry means staining the cells in the sample with markers to see what kind of cells they are. CD34 is a marker for undifferentiated cells. S-100 is another marker, but I can't tell you exactly what it indicates. The presence or absence of markers can mean either good or bad news, but I don't know exactly how these are used here and I don't want to guess.\n\nThat doesn't really answer your questions, because you really need a doctor who knows this case to tell you what to expect. I hope that makes it a little less mysterious, but I'm afraid it's not much help. It makes me sad to think that someone would just hand you this report and not give you some help in understanding what is going on. Sometimes hospitals have a social worker who is there to translate the medical jargon into things you really want and need to know. Ask if there is someone like this. I hope it's a good outcome. Good luck.
627434
"The nutritive composition of a hot aqueous extract of wild Ganoderma fruit bodies was determined. This extract was assessed for cytotoxicity and in vivo genotoxicity by both acute and subchronic exposure of mice (given by mouth at a dose equivalent to extract of 220g fresh Ganoderma fruit body/kg body weight). To test any alleged protection against mutagens by Ganoderma treatments, the mice were injected intraperitoneally with the radiomimetic mutagen ethyl methanesulfonate (EMS), and after 24hr of treatment their lymphocytes were examined using the comet assay. Ganoderma extract consisted of Folin-positive material (68.9% of dry weight), but protein comprised only 7.3% of dry weight. Glucose accounted for 11. 1% and metals 10.2% of dry weight (K, Mg and Ca being the major components with Ge (often touted as being of value in sales literature for Ganoderma preparations) having the fifth highest metal concentration at 489 microg/g). In comparison to rodent chow, Ganoderma extract was a modest dietary supplement. No evidence was found for genotoxic chromosomal breakage nor cytotoxic effects by Ganoderma extract in the mouse, nor did it protect against the effects of ethyl methanesulfonate. We found no support in this study for the extract having any value in protecting against the test mutagen.\nPMID: 10717357, UI: 20183650" (1)\n\nSince the fungus has been used for 2100 years to treat both liver disease and diabetes, and the most effective treatments of both have been discovered only in about the last 50 years, I'd wait until more studies were done, and any healing components of the fungus described, extracted and purified before I'd count on it. Really, they didn't cure hepatitis or diabetes very well 1000 years ago!