title: Gold: Greatest Hits (Steps album)

abstract: Gold: Greatest Hits is the first greatest hits album released by British pop group Steps. It was released in 2001 and reached number one on the UK Albums Chart. The lead single from the album was "Chain Reaction", a cover of the Diana Ross hit; the Steps' version reached number 2 in the UK Singles chart and was the group's highest selling single since "Say You'll Be Mine/Better The Devil You Know". The second single from the album, the slow ballad "Words Are Not Enough", was released with a cover of "I Know Him So Well", from ABBAmania. The single was notably Steps' first single to have an accompanying video that was almost fully animated and did not feature a dance routine like their video for "Heartbeat". The single reached number 5 in the UK charts and was their lowest-selling single to date. A release for "Baby Don't Dance" was planned for 2002 but was scrapped due to the group's split although promotional copies surfaced before the release of The Last Dance.

text:
# Gold: Greatest Hits (Steps album)

Professional ratings
| Review scores | |
| --- | --- |
| Source | Rating |
| AllMusic |  |
| *NME* |  |
| *RTÉ Entertainment* |  |

***Gold: Greatest Hits*** is the first greatest hits album released by British pop group Steps. It was released in 2001 and reached number one on the UK Albums Chart. The lead single from the album was "Chain Reaction", a cover of the Diana Ross hit; the Steps' version reached number 2 in the UK Singles chart and was the group's highest selling single since "Say You'll Be Mine/Better The Devil You Know". The second single from the album, the slow ballad "Words Are Not Enough", was released with a cover of "I Know Him So Well", from *ABBAmania*. The single was notably Steps' first single to have an accompanying video that was almost fully animated and did not feature a dance routine like their video for "Heartbeat". The single reached number 5 in the UK charts and was their lowest-selling single to date. A release for "Baby Don't Dance" was planned for 2002 but was scrapped due to the group's split although promotional copies surfaced before the release of *The Last Dance*.

## Background and release

Steps announced the release of their greatest hits album in May 2001. Shortly after, rumours started surfacing that the group would be splitting up following the release, although these rumours were continuously denied by the group during TV shows and interviews. While discussing the rumours during an interview with whereitsat.tv, band member Lisa Scott-Lee stated "I've got to be honest when I was growing up and I had a lot of my favourite bands bring out greatest hits I thought that, that was the end and "That's their final album" and I'm sure a lot of kids think that and a lot of other people but it's not the case we've actually got lots more albums in the pipeline, we're actually song writing right now for the greatest hits and the fourth album after that, so you know we haven't actually discussed splitting up so I don't see why other people should".

The album was released on 15 October 2001 in the UK.

## Composition

*Gold: Greatest Hits* consists of all the singles that Steps released, except for "You'll Be Sorry". It is notable that the single was not included on the compilation, although "Here and Now" was included. Four new tracks including "Chain Reaction" were featured on the album. A song called "Only in My Dreams" was written by the five members of the group with long-time collaborator Frampton, and was the only song from the album not to be released as a single or to be performed live.

The US version was released under the name *The Best of Steps*. The version of "One for Sorrow" on this album is the original mix, not the US Mix. The US version of the album features three singles that were not released in the US—"Better Best Forgotten", "Chain Reaction", and "Words Are Not Enough". This version of the album did not include all of Steps' singles.

## Commercial performance

Two weeks after the album was released it reached number one in the UK Albums Chart and spent two weeks at the top of the chart. It has sold over a million copies, giving the album a certification of x4 Platinum.

## Tour

To support the release of the album the group embarked on their fifth concert tour (Gold Tour) on 24 November 2001. The tour ended on 22 December 2001 and it was revealed on the TV show *Steps Reunion* that band members  and Claire had announced their resignation to the rest of the group two hours before going on stage for the last show. Lisa and Faye stated that the concert was difficult to perform, while Lee said that he felt the concert cheated the audience.

## Track listing

Standard edition
| No. | Title | Writer(s) | Length |
| --- | --- | --- | --- |
| 1. | "Tragedy" (from *Steptacular*) | Bee Gees | 4:30 |
| 2. | "One for Sorrow" (from *Step One*) |  | 4:21 |
| 3. | "Stomp" (from *Buzz*) | * Topham * Twigg * Rita Campbell * Bernard Edwards * Nile Rodgers | 3:21 |
| 4. | "Better Best Forgotten" (Radio edit, UK track, from *Step One*) |  | 3:42 |
| 5. | "Love's Got a Hold on My Heart" (from *Steptacular*) | * Frampton * Waterman | 3:19 |
| 6. | "Deeper Shade of Blue" (Radio edit, from *Steptacular*) | * Topham * Twigg | 3:44 |
| 7. | "Last Thing on My Mind" (from *Step One*) |  | 3:04 |
| 8. | "Better the Devil You Know" (UK track, from *Buzz*) |  | 3:48 |
| 9. | "Summer of Love" (from *Buzz*) | * Topham * Twigg | 3:51 |
| 10. | "5,6,7,8" (from *Step One*) |  | 3:21 |
| 11. | "Chain Reaction" | Bee Gees | 3:56 |
| 12. | "Baby Don't Dance" |  | 3:50 |
| 13. | "It's the Way You Make Me Feel" (from *Buzz*) | Jörgen Elofsson | 3:16 |
| 14. | "After the Love Has Gone" (from *Steptacular*) | * Topham * Twigg * Ellington | 4:34 |
| 15. | "Here and Now" (Q-Street Mix, UK track, from *Buzz*) |  | 3:26 |
| 16. | "Say You'll Be Mine" (from *Steptacular*) | * Frampton * Waterman | 3:31 |
| 17. | "Only In My Dreams" |  | 4:16 |
| 18. | "Words Are Not Enough" |  | 3:23 |
| 19. | "When I Said Goodbye" (UK track, Single version from *Steptacular*) | * Topham * Twigg | 3:32 |
| 20. | "Heartbeat" (UK track, from *Step One*) | Jackie James | 4:23 |
| Total length: | | | 75:08 |

### US release

*Gold: Greatest Hits* was released in 2002 in the United States as *Best of Steps*.

Standard edition
| No. | Title | Length |
| --- | --- | --- |
| 1. | "Tragedy" | 4:30 |
| 2. | "One for Sorrow" | 4:21 |
| 3. | "Stomp" | 3:21 |
| 4. | "It's the Way You Make Me Feel" | 3:16 |
| 5. | "Love's Got a Hold on My Heart" | 3:19 |
| 6. | "Heartbeat" | 4:23 |
| 7. | "Better Best Forgotten" | 3:42 |
| 8. | "Say You'll Be Mine" | 3:31 |
| 9. | "Deeper Shade of Blue" | 3:44 |
| 10. | "5,6,7,8" | 3:21 |
| 11. | "Last Thing on My Mind" | 3:04 |
| 12. | "Chain Reaction" | 3:56 |
| 13. | "Words Are Not Enough" | 3:23 |

### Digital

Digital 2020 edition
| No. | Title | Length |
| --- | --- | --- |
| 21. | "You'll Be Sorry (The Pardon Mix)" | 3:31 |
| 22. | "I Know Him So Well" | 4:15 |
| 23. | "Merry Xmas Everybody" | 3:08 |
| 24. | "W.I.P. Megamix" | 7:19 |

## Charts

### Weekly charts

| Chart (2001) | Peak
position |
| --- | --- |
| Australian Albums Chart (ARIA) | 155 |
| Irish Albums Chart | 1 |
| Japanese Albums Chart | 92 |
| New Zealand RIANZ Albums Chart | 17 |
| UK Albums Chart | 1 |
|

### Year-end charts

| Chart (2001) | Position |
| --- | --- |
| UK Albums Chart | 6 |
|

## Certifications

| Region | Certification | Certified units/sales |
| --- | --- | --- |
| United Kingdom (BPI) | 4× Platinum | 1,129,620 |

## InfoBox

| Gold: Greatest Hits | |
| --- | --- |
|  | |
| Compilation album by Steps | |
| Released | 15 October 2001 (EU) |
| Recorded | 1997–2001 |
| Genre | Pop |
| Length | 75:08 |
| Label | Jive |
| Producer |  |
| Steps chronology | |
| *Buzz*
(2000) ***Gold: Greatest Hits***
(2001) *The Last Dance*
(2002) | |
|  | |
| Singles from  *Gold: Greatest Hits* | |
| 1. "Chain Reaction"
Released: 24 September 2001 2. "Words Are Not Enough"
Released: 3 December 2001 | |
|  | |


abstract: This is a table of all gold medalists in the Badminton World Championships. Since 1983, the event has been held every two years, with it changing to an annual event since 2005 but taking a break during Olympic years.

text:
# Gold medalists at the BWF World Championships

This is a table of all **gold medalists in the Badminton World Championships**. Since 1983, the event has been held every two years, with it changing to an annual event since 2005 but taking a break during Olympic years.

## Results

| Year | Men's singles | Women's singles | Men's doubles | Women's doubles | Mixed doubles |
| --- | --- | --- | --- | --- | --- |
| 1977 | Flemming Delfs | Lene Køppen | Tjun Tjun
Johan Wahjudi | Etsuko Toganoo
Emiko Ueno | Steen Skovgaard
Lene Køppen |
| 1980 | Rudy Hartono | Verawaty Wiharjo | Ade Chandra
Christian Hadinata | Nora Perry
Jane Webster | Christian Hadinata
Imelda Wiguna |
| 1983 | Icuk Sugiarto | Li Lingwei | Steen Fladberg
Jesper Helledie | Lin Ying
Wu Dixi | Thomas Kihlström
Nora Perry |
| 1985 | Han Jian | Han Aiping | Park Joo-bong
Kim Moon-soo | Han Aiping
Li Lingwei | Park Joo-bong
Yoo Sang-hee |
| 1987 | Yang Yang | | Li Yongbo
Tian Bingyi | Lin Ying
Guan Weizhen | Wang Pengren
Shi Fangjing |
| 1989 | | | | Li Lingwei | Park Joo-bong
Chung Myung-hee |
| 1991 | Zhao Jianhua | | Tang Jiuhong | Park Joo-bong
Kim Moon-soo | Guan Weizhen
Nong Qunhua |
| 1993 | Joko Suprianto | Susi Susanti | Ricky Subagja
Rudy Gunawan | Nong Qunhua
Zhou Lei | Thomas Lund
Catrine Bengtsson |
| 1995 | Hariyanto Arbi | Ye Zhaoying | Rexy Mainaky
Ricky Subagja | Gil Young-ah
Jang Hye-ock | Thomas Lund
Marlene Thomsen |
| 1997 | Peter Rasmussen | | Candra Wijaya
Sigit Budiarto | Ge Fei
Gu Jun | Liu Yong
Ge Fei |
| 1999 | Sun Jun | Camilla Martin | | Ha Tae-kwon
Kim Dong-moon | Kim Dong-moon
Ra Kyung-min |
| 2001 | Hendrawan | Gong Ruina | Halim Haryanto
Tony Gunawan | Gao Ling
Huang Sui | Zhang Jun
Gao Ling |
| 2003 | Xia Xuanze | Zhang Ning | Lars Paaske
Jonas Rasmussen | | Kim Dong-moon
Ra Kyung-min |
| 2005 | Taufik Hidayat | Xie Xingfang | Howard Bach
Tony Gunawan | Yang Wei
Zhang Jiewen | Nova Widianto
Liliyana Natsir |
| 2006 | Lin Dan | | Cai Yun
Fu Haifeng | Gao Ling
Huang Sui | Nathan Robertson
Gail Emms |
| 2007 | | Zhu Lin | Markis Kido
Hendra Setiawan | Zhang Jiewen
Yang Wei | Nova Widianto
Liliyana Natsir |
| 2009 | | Lu Lan | Cai Yun
Fu Haifeng | Zhang Yawen
Zhao Tingting | Thomas Laybourn
Kamilla Rytter Juhl |
| 2010 | Chen Jin | | Wang Lin | Du Jing
Yu Yang | Zheng Bo
Ma Jin |
| 2011 | Lin Dan | | Wang Yihan | Wang Xiaoli
Yu Yang | Zhang Nan
Zhao Yunlei |
| 2013 | | Ratchanok Intanon | | Mohammad Ahsan
Hendra Setiawan | Tontowi Ahmad
Liliyana Natsir |
| 2014 | Chen Long | Carolina Marín | Ko Sung-hyun
Shin Baek-cheol | Tian Qing
Zhao Yunlei | Zhang Nan
Zhao Yunlei |
| 2015 | | | Mohammad Ahsan
Hendra Setiawan | | |
| 2017 | Viktor Axelsen | Nozomi Okuhara | Liu Cheng
Zhang Nan | Chen Qingchen
Jia Yifan | Tontowi Ahmad
Liliyana Natsir |
| 2018 | Kento Momota | Carolina Marín | Li Junhui
Liu Yuchen | Mayu Matsumoto
Wakana Nagahara | Zheng Siwei
Huang Yaqiong |
| 2019 | | P. V. Sindhu | Mohammad Ahsan
Hendra Setiawan | | |
| 2021 | Loh Kean Yew | Akane Yamaguchi | Takuro Hoki
Yugo Kobayashi | Chen Qingchen
Jia Yifan | Dechapol Puavaranukroh
Sapsiree Taerattanachai |
| 2022 | Viktor Axelsen | | Aaron Chia
Soh Wooi Yik | | Zheng Siwei
Huang Yaqiong |
| 2023 | Kunlavut Vitidsarn | An Se-young | Kang Min-hyuk
Seo Seung-jae | | Seo Seung-jae
Chae Yu-jung |

## World Championships of the WBF

| Year | Men's singles | Women's singles | Men's doubles | Women's doubles | Mixed doubles |
| --- | --- | --- | --- | --- | --- |
| 1978 | Yu Yaodong | Zhang Ailing | Yu Yaodong
Hou Jiachang | Zhang Ailing
Li Fang | Pichai Kongsirithavorn
Petchroong Liengtrakulngam |
| 1979 | Han Jian | Han Aiping | Sun Zhian
Yao Ximing | Sirisriro Patama
Suleeporn Jittariyakul | Ng Chun Ching
Chan Lim Chee |


title: Gold of the Seven Saints

abstract: Gold of the Seven Saints is a 1961 American Western film adaptation of a 1957 Steve Frazee novel titled Desert Guns. Released by Warner Brothers, the 88-minute film starred Clint Walker, Roger Moore, Letícia Román, Robert Middleton, and Chill Wills. It was directed by Gordon Douglas, who had earlier directed Walker in 1958's Fort Dobbs and 1959's Yellowstone Kelly. Leigh Brackett wrote the screenplay and Joseph F. Biroc provided the black-and-white photography, most of which was shot in and around Arches National Park in Utah. The film did not do very well at the box office.

text:
# Gold of the Seven Saints

***Gold of the Seven Saints*** is a 1961 American Western film adaptation of a 1957 Steve Frazee novel titled *Desert Guns*. Released by Warner Brothers, the 88-minute film starred Clint Walker, Roger Moore, Letícia Román, Robert Middleton, and Chill Wills. It was directed by Gordon Douglas, who had earlier directed Walker in 1958's *Fort Dobbs* and 1959's *Yellowstone Kelly*. Leigh Brackett wrote the screenplay and Joseph F. Biroc provided the black-and-white photography, most of which was shot in and around Arches National Park in Utah. The film did not do very well at the box office.

## Plot

Two fur traders, Jim Rainbolt (Clint Walker) and Shaun Garrett (Roger Moore), stumble across a big gold strike. With the ruthless bandit McCracken (Gene Evans) and his men in relentless pursuit, they hide the gold behind a giant boulder. Shaun is wounded, but Doc Gates (Chill Wills) shows up out of nowhere and patches him up and is made a full partner. They take refuge at the ranch of Amos Gondora, an old friend of Jim's. There they are introduced to Gondora's so-called "ward", an Indian maiden called Tita (Letícia Román). That night some of McCracken's men stage a stampede and draw Jim and Gondora away. While they are gone, McCracken and some men ride up and take Shaun and Doc captive. He kills Doc because he is unable to tell the hiding place of the gold.

Shaun does not know how to find the hiding place and even under torture cannot lead anyone to the gold. Rainbolt tracks and finds McCracken and his men. After a shootout, McCracken is the only one left of his gang, but he has a gun to Shaun's head and compels Rainbolt to lead him to the gold. When they get there, Rainbolt feigns being unable to move the boulder alone, drawing McCracken in close enough for Rainbolt to roll another boulder onto McCracken's leg, trapping him. Rainbolt and Garrett intend to leave McCracken there to die, but Gondora and his men show up.

Everything seems all right until Gondora puts friendship aside and demands the gold for himself. Jim and Shaun run from them and must cross a rushing river to get away. The bags of gold fall apart and the gold is lost in the river where it came from. After they laugh at this turn of events, Gondora pledges his friendship again and Rainbolt and Garrett set out to return to their fur trapping.

## Cast

## Production

Parts of the film were shot in Professor Valley, Fisher Towers, Arches, Dead Horse Point, Kane Creek Canyon, King's Bottom, Sevenmile Wash, and Klondike Flats in Utah.

## InfoBox

| Gold of the Seven Saints | |
| --- | --- |
| Theatrical release poster | |
| Directed by | Gordon Douglas |
| Screenplay by | Leigh Brackett
Leonard Freeman |
| Based on | *Desert Guns*
1957 novel
by Steve Frazee |
| Produced by | Leonard Freeman |
| Starring | Clint Walker
Roger Moore
Robert Middleton
Chill Wills
Letícia Román |
| Cinematography | Joseph F. Biroc |
| Edited by | Folmar Blangsted |
| Music by | Howard Jackson |
| Production
company | Warner Bros. |
| Distributed by | Warner Bros. |
| Release date | * February 18, 1961 (1961-02-18) |
| Running time | 88 minutes |
| Country | United States |
| Language | English |


abstract: Upfront! Canadians Live from Mountain Stage is an album released in 1994 on Blue Plate Music and BMG Music Canada, which compiles live performances by Canadian artists who have appeared on the American radio series Mountain Stage. The album was intended as a Canadian-only introduction to Mountain Stage, which was not widely broadcast in Canada. The Best of Mountain Stage series of compilation albums, which had already been available in the United States, was subsequently released in Canada.

text:
# Upfront! Canadians Live from Mountain Stage

***Upfront! Canadians Live from Mountain Stage*** is an album released in 1994 on Blue Plate Music and BMG Music Canada, which compiles live performances by Canadian artists who have appeared on the American radio series *Mountain Stage*.

The album was intended as a Canadian-only introduction to *Mountain Stage*, which was not widely broadcast in Canada. The *Best of Mountain Stage* series of compilation albums, which had already been available in the United States, was subsequently released in Canada.

## Track listing

1. Bruce Cockburn, "Waiting for a Miracle"
2. Barenaked Ladies, "What a Good Boy"
3. Crash Test Dummies, "Superman's Song"
4. Cowboy Junkies, "Misguided Angel"
5. Stephen Fearing, "Blue Line"
6. The Rankin Family, "Feir a bhata"
7. Sarah McLachlan, "Shelter"
8. Blue Rodeo, "Already Gone"
9. Spirit of the West, "Political"
10. Rick Danko and Garth Hudson, "Twilight"
11. Moxy Früvous, "The Greatest Man in America"

## InfoBox

| Upfront! Canadians Live from Mountain Stage | |
| --- | --- |
|  | |
| Live album by Various artists | |
| Released | 1994 |
| Label | Blue Plate Music |


title: Upfield Bike Path

abstract: The Upfield Shared Path is a shared use path for cyclists and pedestrians, which follows Upfield railway line through the inner northern suburbs of Melbourne, Victoria, Australia. It stretches from Princes Park, through Brunswick and Coburg, to Box Forest Road, Fawkner, just south of Gowrie railway station. It was originally opened in the late 1980s. Missing links have been progressively filled with the work between Hope Street and Anstey railway station in Brunswick completed in August 2002. A section between Reynard and O'Hea Streets in Coburg with a signalled crossing at Bell Street was completed by 2006 by City of Moreland Council and VicRoads. An extension to Box Forest Road was completed in September 2008. An interactive map of the trail as well as others in the Melbourne area is available here.

text:
# Upfield Bike Path

The **Upfield Shared Path** is a shared use path for cyclists and pedestrians, which follows Upfield railway line through the inner northern suburbs of Melbourne, Victoria, Australia.

It stretches from Princes Park, through Brunswick and Coburg, to Box Forest Road, Fawkner, just south of Gowrie railway station. It was originally opened in the late 1980s.

Missing links have been progressively filled with the work between Hope Street and Anstey railway station in Brunswick completed in August 2002. A section between Reynard and O'Hea Streets in Coburg with a signalled crossing at Bell Street was completed by 2006 by City of Moreland (now City of Merri-bek) Council and VicRoads. An extension to Box Forest Road was completed in September 2008.

An interactive map of the trail as well as others in the Melbourne area is available here.

## Following the path

The southern end of the Upfield Bike Path connects to the Capital City Trail on the old Inner Circle railway line reservation which provides access to the Merri Creek Trail at Rushall railway station; and in the other direction it passes near the Melbourne Zoo before connecting with the Moonee Ponds Creek Trail.

Alternatively, at Park Street cyclists can join the Royal Parade bike path and on road bike lane which provides access to University of Melbourne main campus in Parkville.

City of Moreland Council has reported the number of people counted riding on the Upfield Bike Path and Sydney Road at Park Street has almost quadrupled from 1992 to 2003. The 24-hour weekday average was 804 in February 2008.

The Upfield path parallels Sydney Road shopping strip in Brunswick and Coburg, and is often used by pedestrians and cyclists for shopping, dog walking as well as commuter cycling.

Historic buildings along the path include the Brunswick Baths at Dawson Street and the Railway Hotel (now a Backpackers Hostel) at Albert Street, Brunswick.

## Landmarks

Upfield railway line, Melbourne Zoo.

## Solar lighting at Fawkner Cemetery

The section passing the Fawkner Cemetery was upgraded in February 2009 to include the world's first shared-pathway, solar lighting schema. Stonnington City Council introduced solar lighting on the Gardiners Creek Trail in May 2007. However they did not make use of a color schema as seen on the Upfield Bike path.

Previously the path was without lights and unfriendly to riders. The new array of red LED on the left and white LED on the right, creates a delineated "airport runway" style, visually stunning, lighting grid, providing up to 600 meters of visible pathway for riders, even at the darkest hours. Hazards are indicated using amber LED.

For safety, the lights are mounted flush with the ground where they absorb more than enough energy, stored in their environmentally friendly super capacitors during daylight, to provide up to 14 hours of continuous lighting.

## Connections

The Western Ring Road Trail is 1.4 km to the north, up Sydney Road, from the most northerly end of the trail.

North end at 37°43′09″S 144°57′41″E / 37.719074°S 144.961295°E / -37.719074; 144.961295. South end at 37°46′49″S 144°57′25″E / 37.780251°S 144.957067°E / -37.780251; 144.957067.

## Cultural references

Upfield Bike Path is the name and subject matter of a song by Melbourne indie band *Huon*. The track appears on the compilation album *Kraftworks*.

* *Bike rides around Melbourne* 3rd edition, 2009, Julia Blunden, Open Spaces Publishing, ISBN 978-0-9752333-4-4
* *Bike Paths Victoria* sixth edition, 2004. Edited and published by Sabey & Associates pp132. ISBN 0-9579591-1-7

## InfoBox

| Upfield Rail Trail | |
| --- | --- |
| The Upfield Bike Path in Brunswick | |
| Length | Approx 8.6 km |
| Location | Melbourne, Victoria, Australia |
| Difficulty | Easy |
| Hazards | Numerous Road crossings. People exiting stations. Forklifts. |
| Surface | Bitumen path |
| Hills | Flat |
| Train(s) | Upfield railway line |


title: Mons Hill

abstract: Mons Hill is a hill situated in Dudley, West Midlands, England. It is a wooded hill which straddles the border of Dudley and Sedgley, standing to the north of the much more famous Wren's Nest hill. It was originally situated within the borders of Sedgley, but to allow the development of nearby land as the Priory Estate in 1926 the borders were altered and the hill has since been within the boundaries of Dudley. From 1965 until 1990, Mons Hill gave its name to a neighbouring secondary school which is now part of Dudley College. Its shared name with Mons Road in the Kates Hill area of Dudley is a coincidence, as that road's name was taken from the Battle of Mons during World War I.

text:
# Mons Hill

**Mons Hill** is a hill situated in Dudley, West Midlands, England.

It is a wooded hill which straddles the border of Dudley and Sedgley, standing to the north of the much more famous Wren's Nest hill.

It was originally situated within the borders of Sedgley (then in the county of Staffordshire), but to allow the development of nearby land as the Priory Estate in 1926 the borders were altered and the hill has since been within the boundaries of Dudley (then in Worcestershire).

From 1965 until 1990, Mons Hill gave its name to a neighbouring secondary school which is now part of Dudley College.

Its shared name with Mons Road in the Kates Hill area of Dudley is a coincidence, as that road's name was taken from the Battle of Mons during World War I.

*

52°31′48″N 2°05′42″W / 52.53°N 2.095°W / 52.53; -2.095


title: Monohalomethane

abstract: The monohalomethanes are organic compounds in which a hydrogen atom in methane is replaced by a halogen. They belong to the haloalkanes or to the subgroup of halomethanes. The four common members are fluoromethane, chloromethane, bromomethane and iodomethane. Historical name for this group is methyl halides; it's still widely used. The compounds of this class are often described as CH3X or MeX.

text:
# Monohalomethane

| Structural formula |  |  |  |  |
| --- | --- | --- | --- | --- |
| Name | Fluoromethane
Methyl fluoride | Chloromethane
Methyl chloride | Bromomethane
Methyl bromide | Iodomethane
Methyl iodide |
| Melting point | −137,8 °C | −97,4 °C | −93,7 °C | −66 °C |
| Boiling point | −78,4 °C | −23,8 °C | 4,0 °C | 42 °C |
| Space-filling model |  |  |  |  |

The **monohalomethanes** are organic compounds in which a hydrogen atom in methane is replaced by a halogen. They belong to the haloalkanes or to the subgroup of halomethanes.

The four common members are fluoromethane, chloromethane, bromomethane and iodomethane.

Historical name for this group is **methyl halides**; it's still widely used. The compounds of this class are often described as CH3X or MeX (X - any halogen, Me - methyl group).

There are analogs with more than one hydrogen atom in methane is replaced by a halogen:

Analogs with carbon atom replaced with a heavier group 14 element are also known:

1. Record of *Fluoromethane* in the GESTIS Substance Database of the Institute for Occupational Safety and Health, accessed on 2020-02-29.
2. Record of *Chloromethane* in the GESTIS Substance Database of the Institute for Occupational Safety and Health, accessed on 2020-02-29.
3. Record of *Bromomethane* in the GESTIS Substance Database of the Institute for Occupational Safety and Health, accessed on 2020-02-29.
4. Record of *Iodomethane* in the GESTIS Substance Database of the Institute for Occupational Safety and Health, accessed on 2020-02-29.
5. "Chemical Fact Sheet: Astatine". European Virtual Institute for Speciation Analysis (EVISA).


title: Monohon, Washington

abstract: Monohon was a town located on the east side of Lake Sammamish, near the present-day intersection of East Lake Sammamish Parkway and SE 33rd Street in the city of Sammamish. The community was originally part of a town named Donnelly, founded by Simon Donelly who built a sawmill there, but then grew big enough and was far enough away from Donelly that residents created a separate town, Monohon, in 1888. The new town was named after Martin Monohon who had homesteaded 160 acres (0.65 km⁲) there in 1877. The railroad along the east side of the lake was completed in 1889, and the Donnelly mill was moved to the site of Monohon. By 1900, there were twenty homes in Monohon, and the lumber mill was updated with the latest machinery. The mill also completed a new water system for the community. This brought both new wealth and new settlers to the community, and by 1911, the town's population had reached over 300. A 20-room hotel was built overlooking the lake, along with a church and a community meeting hall. The dock was used to ship lumber and dairy products on the lake. During the height of the prohibition era, the town was raided by King County Sheriff officers looking for bootleggers. 50 gallons of moonshine whiskey were reportedly confiscated. In 1925, the town was largely destroyed by a fire, with only about 10 homes surviving. The mill was rebuilt, but the town never recovered, and all but disappeared when the Great Depression hit in 1929. The sawmill continued to operate for many years after, but was repeatedly burned and rebuilt. The mill finally closed permanently in 1980.

text:
# Monohon, Washington

**Monohon** was a town located on the east side of Lake Sammamish (then known as Lake Squak), near the present-day intersection of East Lake Sammamish Parkway and SE 33rd Street in the city of Sammamish. The community was originally part of a town named Donnelly, founded by Simon Donelly who built a sawmill there, but then grew big enough and was far enough away from Donelly that residents created a separate town, Monohon, in 1888. The new town was named after Martin Monohon who had homesteaded 160 acres (0.65 km2) there in 1877. The railroad along the east side of the lake was completed in 1889, and the Donnelly mill was moved to the site of Monohon.

By 1900, there were twenty homes in Monohon, and the lumber mill was updated with the latest machinery. The mill also completed a new water system for the community. This brought both new wealth and new settlers to the community, and by 1911, the town's population had reached over 300. A 20-room hotel was built overlooking the lake, along with a church and a community meeting hall. The dock was used to ship lumber and dairy products on the lake.

During the height of the prohibition era, the town was raided by King County Sheriff officers looking for bootleggers. 50 gallons of moonshine whiskey were reportedly confiscated.

In 1925, the town was largely destroyed by a fire, with only about 10 homes surviving. The mill was rebuilt, but the town never recovered, and all but disappeared when the Great Depression hit in 1929. The sawmill continued to operate for many years after, but was repeatedly burned and rebuilt. The mill finally closed permanently in 1980.

## InfoBox

| Monohon, Washington | |
| --- | --- |
| Ghost town | |
| Monohon and its sawmill, circa 1910 | |
| MonohonMonohon | |
| Coordinates: 47°34′47″N 122°04′29″W / 47.57972°N 122.07472°W / 47.57972; -122.07472 | |
| Country | United States |
| State | Washington |
| County | King |
| Time zone | UTC-8 (Pacific (PST)) |
| Summer (DST) | UTC-7 (PDT) |


title: Godojos

abstract: Godojos is a municipality located in the province of Zaragoza, Aragon, Spain. According to the 2004 census (INE), the municipality has a population of 60 inhabitants.

text:
# Godojos

**Godojos** is a municipality located in the province of Zaragoza, Aragon, Spain. According to the 2004 census (INE), the municipality has a population of 60 inhabitants.

## InfoBox

| Godojos | |
| --- | --- |
| municipality | |
| Sierra de Solorio rising behind Godojos | |
| GodojosGodojosGodojos | |
| Coordinates: 41°16′N 1°52′W / 41.267°N 1.867°W / 41.267; -1.867 | |
| Country | Spain |
| Autonomous community | Aragon |
| Province | Zaragoza |
| Area | |
| Total | 16 km2 (6 sq mi) |
| Population (2018) | |
| Total | 54 |
| Density | 3.4/km2 (8.7/sq mi) |
| Time zone | UTC+1 (CET) |
| Summer (DST) | UTC+2 (CEST) |


title: University of Patanjali

abstract: University of Patanjali is an Indian private research university, with its main campus in Haridwar, Uttarakhand. It was established in 2006 after the Legislative Assembly of Uttarakhand passed the University of Patanjali Act, 2006. It was founded by Baba Ramdev and Acharya Balkrishna.

text:
# University of Patanjali

**University of Patanjali** is an Indian private research university, with its main campus in Haridwar, Uttarakhand. It was established in 2006 after the Legislative Assembly of Uttarakhand passed the University of Patanjali Act, 2006. It was founded by Baba Ramdev and Acharya Balkrishna.

## InfoBox

University of Patanjali
| Seal | |
| --- | --- |
| Type | Private |
| Established | 2006 (2006) |
| Vice-Chancellor | Acharya Balkrishna |
| Location | Haridwar, Uttarakhand, India<br>29°54′31″N 77°59′59″E / 29.9085°N 77.9997°E / 29.9085; 77.9997 |
| Campus | Urban, 35 acres (14 ha) |
| Colours | Red and Yellow |
| Affiliations | UGC, AICTE |
| Website | www.universityofpatanjali.com |


title: University of Paris-Seine

abstract: University of Paris-Seine was an association of universities and higher education institutions (ComUE) located in the region of Paris, France. Member institutes include National School of Arts (Paris-Cergy), National School of Architecture (Versailles), National School of Landscaping, ESSEC Business School and University of Cergy-Pontoise among others. Started as the Paris-Seine initiative, it was renamed to University of Paris-Seine in 2015 according to the 2013 Law on Higher Education and Research (France).

text:
# University of Paris-Seine

**University of Paris-Seine** was an association of universities and higher education institutions (ComUE) located in the region of Paris, France. Member institutes include National School of Arts (Paris-Cergy), National School of Architecture (Versailles), National School of Landscaping, ESSEC Business School and University of Cergy-Pontoise among others. Started as the Paris-Seine initiative, it was renamed to University of Paris-Seine in 2015 according to the 2013 Law on Higher Education and Research (France).

## History

The association original formed as one of the Pôle de recherche et d'enseignement supérieur (PRES) in France, organizing universities and research institutions from West of Île-de-France, covering nearly the whole spectrum of sciences (fundamental, engineering, business). As a PRES, the association was named "Université du grand ouest parisien" and was officially created the 6th of February 2012, in the form of a *établissement public de coopération scientifique* (EPCS). It has been renamed « Université Paris-Seine » the 1st of March 2015.

## Members

The members of the University of Paris-Seine as of the 2015 statute are:

48°30′00″N 2°30′00″E / 48.5000°N 2.5000°E / 48.5000; 2.5000

## InfoBox

University of Paris-Seine
| Université Paris-Seine | |
| --- | --- |
|  | |
| Type | Public |
| Active | 6th of February 2012–1st of January 2020 |
| President | Anne-Sophie Barthez |
| Location | Île-de-France, France |
| Nickname | UPS |
| Website | http://www.universiteparisseine.fr |


title: Monte Tagliaferro

abstract: Monte Tagliaferro is a mountain of the Pennine Alps. It is one of the most popular hiking destination of Valsesia.

text:
# Monte Tagliaferro

**Monte Tagliaferro** is a mountain of the Pennine Alps. It is one of the most popular hiking destination of Valsesia.

## Etymology

The name, which literally means *iron cutting*, may come from a sharp stone block located not far away from the top.

## Geography

The mountain is located in Valsesia (VC, Piemonte region). Administratively it is divided between the comunes of Alagna Valsesia and Alto Sermenza.

### SOIUSA classification

According to the SOIUSA (*International Standardized Mountain Subdivision of the Alps*) the mountain can be classified in the following way:

* main part = Western Alps
* major sector = North Western Alps
* section = Pennine Alps
* subsection = Eastern Aosta and Northern Valsesia Alps
* supergroup = Contrafforti valsesiani del Monte Rosa
* group = Costiera Punta Grober-Tagliaferro-Montevecchio
* subgroup = Costiera del Tagliaferro
* sector of subgroup = Contrafforte Piglimò-Tagliaferro
* code = I/B-9.III-C.7.b/a

## Access to the summit

The easiest route for the summit starts from Rima San Giuseppe and reaches the summit passing through Vallarolo pass (2,332  m). Nearby the mountain, at 2,264  m, is located *Rifugio Ferioli*, a public mountain hut.

## Maps

## InfoBox

| Monte Tagliaferro | |
| --- | --- |
| NW face | |
| Highest point | |
| Elevation | 2,964 m (9,724 ft) |
| Prominence | 637 m (2,090 ft) |
| Isolation | 4.16 km (2.58 mi) |
| Listing | Alpine mountains 2500-2999 m |
| Coordinates | 45°52′20″N 07°58′13″E / 45.87222°N 7.97028°E / 45.87222; 7.97028 |
| Geography | |
| Monte TagliaferroLocation in the Alps | |
| Location | Province of Vercelli, northern Italy |
| Parent range | Pennine Alps |
| Climbing | |
| Easiest route | from Rima San Giuseppe |


title: Monte Tabor metro station

abstract: Monte Tabor is a metro station on the Line 5 of the Santiago Metro, in Santiago, Chile. The station was opened on 3 February 2011 as part of the extension of the line from Pudahuel to Plaza de Maipú. It is one of the three stations built on a viaduct on the just mentioned extension. The platforms and tracks are enclosed by a steel-framed structure. The portal frames are rectangular in shape with rounded corners and have different heights, which creates an undulating pattern along the platforms lengthwise. The structure base is overlapped by an arched tensile membrane structure. This portion of the station is laterally covered with sandwich-structured composite panels of aluminum. The station features a double decker skyway, which connects the platforms to a cylindrical building. The walkway on the lower deck leads to the westbound platform, while the higher walkway crosses over the tracks and connects with the eastbound platform via two stairways and a two-stop elevator.

text:
# Monte Tabor metro station

**Monte Tabor** is a metro station on the Line 5 of the Santiago Metro, in Santiago, Chile. The station was opened on 3 February 2011 as part of the extension of the line from Pudahuel to Plaza de Maipú. It is one of the three stations built on a viaduct on the just mentioned extension.

The platforms and tracks are enclosed by a steel-framed structure. The portal frames are rectangular in shape with rounded corners and have different heights, which creates an undulating pattern along the platforms lengthwise. The structure base is overlapped by an arched tensile membrane structure. This portion of the station is laterally covered with sandwich-structured composite panels of aluminum.

The station features a double decker skyway, which connects the platforms to a cylindrical building. The walkway on the lower deck leads to the westbound platform, while the higher walkway crosses over the tracks and connects with the eastbound platform via two stairways and a two-stop elevator.

## InfoBox

| Monte Tabor | |
| --- | --- |
| **Santiago Metro station** | |
|  | |
| General information | |
| Location | Pajaritos Avenue / Monte Tabor Street |
| Coordinates | 33°28′56.07″S 70°44′43.58″W / 33.4822417°S 70.7454389°W / -33.4822417; -70.7454389 |
| Line(s) | Line 5 |
| Platforms | 2 side platforms |
| Tracks | 2 |
| Connections | Transantiago buses |
| Construction | |
| Accessible | Yes |
| History | |
| Opened | February 3, 2011 |
|  | |
| Services | |
| Preceding station Santiago Metro Following station  Del Soltowards Plaza de Maipú  Line 5  Las Parcelastowards Vicente Valdés | |
|  | |
|  | |
|  | |


title: Monte Tamaro (ship)

abstract: Monte Tamaro is a container ship owned by A.P. Moller Singapore Pte. Ltd. and operated by Maersk Line AS. The 272-metre (892 ft) long ship was built at Daewoo Shipbuilding & Marine Engineering in Okpo, South Korea in 2007. Originally owned by Monte Tamaro GmbH & Co KG, a subsidiary of Hamburg Süd, she has had two owners and been registered under three flags. The vessel is one of ten ships of the Monte class built for Hamburg Süd by Daewoo Shipbuilding & Marine Engineering and Daewoo Mangalia Heavy Industries between 2004 and 2009.

text:
# Monte Tamaro (ship)

**Monte Tamaro** is a container ship owned by A.P. Moller Singapore Pte. Ltd. and operated by Maersk Line AS. The 272-metre (892 ft) long ship was built at Daewoo Shipbuilding & Marine Engineering in Okpo, South Korea in 2007. Originally owned by Monte Tamaro GmbH & Co KG, a subsidiary of Hamburg Süd, she has had two owners and been registered under three flags.

The vessel is one of ten ships of the *Monte* class built for Hamburg Süd by Daewoo Shipbuilding & Marine Engineering and Daewoo Mangalia Heavy Industries between 2004 and 2009.

## Construction

*Monte Tamaro* had its keel laid down on 26 June 2007 at Daewoo Shipbuilding & Marine Engineering in Okpo, South Korea. Its hull has an overall length of 272 metres (892 ft). In terms of width, the ship has a beam of 40 metres (130 ft). The height from the top of the keel to the main deck, called the moulded depth, is 24.2 metres (79 ft).

The ship's container-carrying capacity of 5,552 twenty-foot equivalent units (TEU) (5,552 20-foot shipping containers) places it in the range of a Post-Panamax container ship. The ship's gross tonnage, a measure of the volume of all its enclosed spaces, is 69,132. Its net tonnage, which measures the volume of the cargo spaces, is 34,823. Its total carrying capacity in terms of weight, is 71,587.5 long tons deadweight (DWT).

The vessel was built with a HSD Engine Co. Ltd. 8RTA96C-B main engine, which drives a fixed-pitch propeller. The 8-cylinder engine has a Maximum Continuous Rating of 45,760 kW with 102 revolutions per minute at MCR. The cylinder bore is 960mm. The ship also features 4 main power distribution system auxiliary generators, 3 at 4,100-kilowatt (5,500 hp), and 1 at 2,700-kilowatt (3,600 hp). The vessel's steam piping system features an Aalborg CH 8-500 auxiliary boiler.

Construction of the ship was completed on 30 November 2007. As of 2018, the ship is classified by the ABS with the code "A1, Container Carrier, AMS, ACCU; RRDA, BWE, Ice Class D0, UWILD, PMP", meaning that it was constructed under the supervision of a recognized classification society, that the construction complies with the society's rules, and that it is classed as a general cargo carrier and container ship.

* Det Norske Veritas (January 2011). "Part 1, Chapter 2: Class Notations". *Rules for the Classification of Ships* (PDF). Høvik, Norway: Det Norske Veritas AS. Archived from the original (PDF) on 2012-01-21. Retrieved 2011-08-05.
* International Maritime Organization (2002). "International Convention on Tonnage Measurement of Ships, 1969". International Maritime Organization. Archived from the original on 2008-01-16. Retrieved 2008-03-23.
* United Nations Conference on Trade and Development (UNCTAD) (2008). *Review of Maritime Transport, 2008* (PDF). New York and Geneva: United Nations. ISBN 978-92-1-112758-4.

## InfoBox

| Container ship *Monte Tamaro* seen from Santos in 2018 | |
| --- | --- |
| History | |
| Singapore | |
| Name | 2018–present: *Monte Tamaro* |
| Owner | A.P. Moller Singapore Pte. Ltd. |
| Operator | Maersk Line AS |
| Port of registry | Singapore as of 5 April 2018 |
| Route | Hamburg Süd North America East Coast - South America East Coast (ASUS) liner service |
| Identification |  |
| Status | In service |
| Portugal | |
| Name | 2015–present: *Monte Tamaro* |
| Owner | 2007-2018: Monte Tamaro GmbH & Co KG |
| Operator | Columbus Shipmanagement GmbH C/O Hamburg Suedamerikanische Dampfschiffahrts-Gesellschaft KG |
| Port of registry | Madeira, Portugal as of 10 January 2015 |
| Germany | |
| Name | 2007–present: *Monte Tamaro* |
| Owner | 2007-2018: Monte Tamaro GmbH & Co KG |
| Operator | Columbus Shipmanagement GmbH C/O Hamburg Suedamerikanische Dampfschiffahrts-Gesellschaft KG |
| Port of registry | Germany as of 11 January 2007 |
| Builder | Daewoo Shipbuilding & Marine Engineering |
| Laid down | 26 June 2007 |
| Launched | 22 September 2007 |
| Completed | 30 November 2007 |
| Identification | IMO number: 9357949 |
| General characteristics | |
| Tonnage |  |
| Length | 272 m (892.4 ft) |
| Beam | 40 m (131.2 ft) |
| Depth | 24.2 m (79.4 ft) |
| Ice class | D0 |
| Installed power | HSD Engine Co. Ltd. 8RTA96C-B |
| Speed | 23 knots |


title: Hank (2009 TV series)

abstract: Hank is an American sitcom that ran on ABC from September 30, 2009, to November 4, 2009. The series was created by Tucker Cawley and is about a Wall Street executive who loses his job and reconnects with his small-town family. Hank originally aired on Wednesday nights in the fall season and starred Frasier actor Kelsey Grammer. The series was produced by McMonkey Productions and Werner Entertainment, in association with Warner Bros. Television.

text:
# Hank (2009 TV series)

***Hank*** (originally titled ***Awesome Hank*** and ***PRYORS***) is an American sitcom that ran on ABC from September 30, 2009, to November 4, 2009. The series was created by Tucker Cawley and is about a Wall Street executive who loses his job and reconnects with his small-town family. *Hank* originally aired on Wednesday nights in the fall season and starred *Frasier* actor Kelsey Grammer. The series was produced by McMonkey Productions and Werner Entertainment, in association with Warner Bros. Television.

## Cast

## Episodes

A total of 10 episodes of *Hank* were filmed and five have been aired, but on November 11, 2009, ABC said it had "no immediate plans" to air the others.

| No. | Title | Directed by | Written by | Original air date | Production
code | U.S. Viewers
(millions) |
| --- | --- | --- | --- | --- | --- | --- |
|
| 1 | "Pilot" | Andrew D. Weyman | Tucker Cawley | September 30, 2009 (2009-09-30) | 2J5341 | 8.336 |
| A legendary entrepreneur in the sports retail world, Hank Pryor, and his wife, Tilly, have been living the high life in New York City. That is until Hank is forced out of his CEO job and has to downsize and move his family back home to the small town of River Bend, Virginia. | | | | | | |
| 2 | "Yard Sale" | Kelsey Grammer | Bill Martin & Mike Schiff | October 7, 2009 (2009-10-07) | 2J5302 | 6.446 |
| Hank gets back into business by testing his entrepreneurial skills with a neighborhood yard sale. | | | | | | |
| 3 | "Drag Your Daughter to Work Day" | Andrew D. Weyman | Tucker Cawley | October 14, 2009 (2009-10-14) | 2J5301 | 6.153 |
| When Hank tells Maddie that she needs to get a job like he used to have at her age, he ends up having to prove what he said was true by taking a job alongside her at the mall. | | | | | | |
| 4 | "Relax, Don't Do It" | Andrew D. Weyman | Jackie Filgo & Jeff Filgo | October 21, 2009 (2009-10-21) | 2J5303 | 5.775 |
| Hank finds it challenging to "do nothing" while unemployed and is the ultimate over-achiever in his roles as a house-husband as well as a hunter | | | | | | |
| 5 | "Hanksgiving" | Andrew D. Weyman | Jackie Filgo & Jeff Filgo | November 4, 2009 (2009-11-04) | 2J5308 | 5.093 |
| Hank's worst holiday nightmare comes true when his mother-in-law comes to visit for the first annual suburban Pryor Family "Hanksgiving". | | | | | | |
| 6 | "Got It" | Kelsey Grammer | Ilana Wernick | January 15, 2013 (2013-01-15) (SBT) | 2J5304 | N/A |
| Hank has had it with Tilly's annoying habit of saying "got it, got it, got it," but is afraid to discuss the issue directly with her, so he recruits his ever-so-subtle brother-in-law, Grady, to approach her about the problem. | | | | | | |
| 7 | "Hank's Got a Friend" | Andrew D. Weyman | DJ Nash | January 22, 2013 (2013-01-22) (SBT) | 2J5305 | N/A |
| Hank gets more than he bargained for when he finds a friend in suburbia. | | | | | | |
| 8 | "The Pryor Name" | Andrew D. Weyman | Steve Skrovan | February 2, 2013 (2013-02-02) (SBT) | N/A | N/A |
| Hank gears up for his first big meeting since his career 'downsized'. | | | | | | |
| 9 | "Tree House" | Andrew D. Weyman | Tucker Cawley & DJ Nash | January 29, 2013 (2013-01-29) (SBT) | N/A | N/A |
| Henry helps with a tree house, while Maddie is skipping her chores. | | | | | | |
| 10 | "Dog" | Gail Mancuso | Bill Martin & Mike Schiff | February 19, 2013 (2013-02-19) (SBT) | N/A | N/A |
| When Henry chooses a grizzled old hound for a pet, Hank realizes he has a lot in common with the doddery old dog. | | | | | | |

## U.S. ratings

| Episode Number | Episode | Rating | Share | Rating/Share
(18–49) | Viewers
(millions) | Rank
(Timeslot) | Rank
(Night) |
| --- | --- | --- | --- | --- | --- | --- | --- |
| **1** | "Pilot" | 5.4 | 9 | 2.2/7 | 8.46 | 4 | 10 |
| **2** | "Yard Sale" | 4.4 | 7 | 1.6/5 | 6.72 | 6 | 14 |
| **3** | "Drag Your Daughter to Work Day" | 4.2 | 7 | 1.6/5 | 6.24 | 6 | 14 |
| **4** | "Relax, Don't Do It" | 3.9 | 7 | 1.5/5 | 5.84 | 6 | 13 |
| **5** | "Hanksgiving" | 3.6 | 6 | 1.4/4 | 5.33 | 7 | 13 |
| **6** | "Got It" | TBA | TBA | TBA | TBA | TBA | TBA |
| **7** | "Hank's Got a Friend" | TBA | TBA | TBA | TBA | TBA | TBA |
| **8** | "The Pryor Name" | TBA | TBA | TBA | TBA | TBA | TBA |
| **9** | "Tree House" | TBA | TBA | TBA | TBA | TBA | TBA |
| **10** | "Dog" | TBA | TBA | TBA | TBA | TBA | TBA |

## Reception

The show received negative reviews from critics, scoring a 36/100 in Metacritic, with viewers agreeing with the critics, scoring it at a 4.1/10. The *Los Angeles Times* has noted "There's nothing here you couldn't imagine from the premise, but there's also nothing wrong with what's here: McGraw is a good foil for Grammer, and Grammer is good at what he does." The *New York Post* panned the show: "*Hank* is one of the worst new (or old) comedies of this or many other seasons".

## Cancellation

*Hank* was officially cancelled on November 11, 2009 amidst low ratings and bad reviews. A total of ten episodes of the series have been produced, leaving five episodes unaired. ABC stated that they had no immediate plans to air the remaining episodes. Special programs and reruns of comedy shows aired in its place. In a December 2009 interview with Jay Leno, Kelsey Grammer admitted that he had initially called Warner Bros. to ask that the show be cancelled because it was too unfunny. The unaired episodes have since aired in the UK on BT Vision and in Croatia on Doma TV.

Official Twitter handle is @hankeisam

## InfoBox

| *Hank* | |
| --- | --- |
| Title screen depicting the series' cast | |
| Also known as | Awesome Hank
PRYORS |
| Genre | Sitcom |
| Created by | Tucker Cawley |
| Starring | Kelsey Grammer
David Koechner
Melinda McGraw
Jordan Hinson
Nathan Gamble |
| Composer | Matter Music |
| Country of origin | United States |
| Original language | English |
| No. of seasons | 1 |
| No. of episodes | 10 (5 unaired in the U.S.) |
| Production | |
| Executive producers | Tucker Cawley
Kelsey Grammer
Mike Clements
Tom Werner |
| Camera setup | Multi-camera |
| Running time | 30 minutes |
| Production companies | McMonkey Productions
Grammnet Productions
Werner Entertainment
Bonanza Productions
Warner Bros. Television |
| Original release | |
| Network | ABC |
| Release | September 30 (2009-09-30)
November 4, 2009 (2009-11-04) |


title: Matters (band)

abstract: Matters was a Canadian indie rock band from Guelph, Ontario. The band consisted of Tim Bruton (guitar/synth), Kyle Donnelly (bass), John O'Regan and Greg Santilly (drums). They played rock music that had elements of punk, dance, and art rock, and used multiple transitions and hooks rather than traditional verse/chorus song structures.

text:
# Matters (band)

**Matters** was a Canadian indie rock band from Guelph, Ontario. The band consisted of Tim Bruton (guitar/synth), Kyle Donnelly (bass), John O'Regan (lead vocals/guitar/keyboard) and Greg Santilly (drums). They played rock music that had elements of punk, dance, and art rock, and used multiple transitions and hooks rather than traditional verse/chorus song structures.

## History

### The D'Urbervilles

Formed in 2005 as **The D'Urbervilles** while the members were students at the University of Guelph, the band originally consisted of O'Regan, Bruton, Donnelly, and drummer C.L. Smith. Prior to Smith's departure, the band independently released their debut EP *The D'Urbervilles*, which reached No. 37 on *earshot!*'s Canadian campus and community radio charts for the month of May 2006. The D'Urbervilles toured and recorded their debut album, *We Are the Hunters*, with replacement drummers Steve Hesselink and Adam Seward before selecting Santilly as a permanent member in the fall of 2007. *We Are the Hunters* was released on the Toronto label Out of This Spark on February 19, 2008 and hit No. 13 on the *earshot!* charts for the month of March. The band completed work on their second full-length, although they have gone on record as saying it's unlikely to be released.

As the D'Urbervilles, the group has toured alongside Malajube, You Say Party! We Say Die!, Immaculate Machine and Forest City Lovers in addition to performing at Pop Montreal, SXSW, Ottawa Bluesfest, and NXNE.

### Change of name

The band changed their name to Matters in March 2011 to signal their shift in musical direction and visual style.

## Discography

### Singles

### Albums

* *The D'Urbervilles* \- Independent - 2006.
* *We Are the Hunters* \- Out of This Spark \- 2008.

### Compilations

## Member associated acts

## InfoBox

| Matters | |
| --- | --- |
| Origin | Guelph, Ontario |
| Genres | Indie rock |
| Years active | 2005 (2005)2011 (2011) |
| Labels | Out of This Spark
Hype Lighter |
|  | |
| Members | Tim Bruton
Kyle Donnelly
John O'Regan
Greg Santilly |
|  | |
| Past members | Adam Seward
Steve Hesselink
C.L. Smith |
|  | |
| Website | officialmatters.org |


title: Wakeham

abstract: Wakeham is a hamlet near the village of Easton, in Tophill on the Isle of Portland in Dorset, England. It is situated between the Straits part of Easton, and Pennsylvania Castle. As with the rest of Portland's villages and settlements, Wakeham has been designated as a conservation area, as it is a place of special architectural and historic interest. Easton, Wakeham and Reforne were designated pre-1974. The hamlet features a distinctively wide road running through it, once built to allow space for horse-drawn carts transporting stone by road. Many of Wakeham's older buildings of the 17th and 18th century survive.

text:
# Wakeham

**Wakeham** is a hamlet near the village of Easton, in Tophill on the Isle of Portland in Dorset, England. It is situated between the Straits part of Easton, and Pennsylvania Castle. As with the rest of Portland's villages and settlements, Wakeham has been designated as a conservation area, as it is a place of special architectural and historic interest. Easton, Wakeham and Reforne were designated pre-1974. The hamlet features a distinctively wide road running through it, once built to allow space for horse-drawn carts transporting stone by road. Many of Wakeham's older buildings of the 17th and 18th century survive.

## History

Wakeham was established around a natural watercourse and various springs, which the Romans are likely to have developed. Among the archaeological finds of Wakeham are Roman stone sarcophagi, and Iron Age underground chambers. The original settlement was situated within the small valley at the south of the hamlet, and this land later became part of the grounds of Pennsylvania Castle. In 789 AD, the first recorded Viking attack within British Isles, including Ireland, occurred on Portland's coast. It is believed that Church Ope Cove was the location, and at the time would have shown Wakeham above it. The exposed location was later defended by the Norman 12th-century Rufus Castle. Wakeham was home to Portland's first parish church, St Andrews Church, which was probably first established above the cove by the Saxons, and rebuilt in the 12th century. The church was later replaced in the 18th century by the new parish church St George's. During the construction of the new church, between 1754 and 1766, a temporary place of worship was established within Wakeham.

Between 1797 and 1800, John Penn, Governor of Portland and grandson of William Penn, the founder of Pennsylvania, had the Gothic revival mansion Pennsylvania Castle built on a platform above the cove, and at the bottom of Wakeham. In order to expand his land, he had Southwell Road diverted, and purchased a number of cottages at the bottom of Wakeham. From the 1840s onwards, Portland saw a large increase within its population, due to the construction of Portland Harbour's Breakwaters. With the need for housing, and the expansion of Portland's villages, Wakeham became part of Easton village. After the First World War, a number of inland quarries were opened around Wakeham area. Opposite Portland Museum at the bottom of Wakeham, a small quarry was opened to provide stone for the Whitehall Cenotaph. The museum was opened in 1930, after Marie Stopes donated two houses for a museum. Today Wakeham remains part of Easton, and is largely residential.

## Commercial business

Unlike the majority of Portland's villages, Wakeham has little commercial business in the area. Accommodation in Wakeham village is a leading business and includes The Alessandria Hotel, the self-contained holiday let Church Ope Studio, and the bed and breakfast Leam Guest House. The Cove Holiday Park is found on the outskirt of Wakeham. The Mermaid Inn, now closed, was once open in the area. In 2010, work was successful in turning the pub into a house.

## Grade listed features

Wakeham has a wide array of architecture and buildings, a number of which are Grade Listed.

Tudor Cottage (167 Wakeham), 203 Wakeham, along with its attached outbuilding, 209 and 211 Wakeham, 99 Wakeham, 95 Wakeham, 205 and 207 Wakeham, 97 Wakeham, 213 Wakeham, 65 Wakeham, 106 Wakeham, Woodbine Cottage (112 Wakeham), 6 Wakeham, 127 Wakeham, 137 and 139 Wakeham, 129 and 131 Wakeham, 116 Wakeham, 114 Wakeham, 118 Wakeham, 215 Wakeham, 79 Wakeham, and 81 Wakeham are all Grade II Listed. A dovecote, approximately 5 metres west-south-west of 106 Wakeham, is also Grade II Listed.

Portland Museum is located within Wakeham and close to Church Ope Cove. The museum is housed in two former cottages, one titled Avice's Cottage, and both of which became Grade II Listed in January 1951. With both retaining thatched roofs, the cottages act as a reminder of the type of cottage that once appeared all across the island. Avice's Cottage was an inspiration within the novel *The Well-Beloved*, written by Thomas Hardy, as the home of three generations of "Avice's" – the novel's heroines.

### Church Ope Cove

Although not quite a part of Wakeham, near to Portland Museum, and above Church Ope Cove is Rufus Castle – a ruined castle dating from the late 15th century, on the site of an earlier building. It has been Grade I Listed since January 1951. In addition to this, the castle has become a scheduled monument under the Ancient Monuments and Archaeological Areas Act 1979. St Andrew's Church sits between the castle and the cove. It has been Grade II\* Listed since the same time as the castle. Pennsylvania Castle is found just on the edge of Wakeham, near Church Ope Cove. The castle and its attached walls has been Grade II Listed since January 1951. The gatehouse and lodges to the castle were also made Grade II Listed at the same time, although the lodges are now separated in ownership from the castle.

## InfoBox

| Wakeham | |
| --- | --- |
| WakehamLocation within Dorset | |
| Civil parish | * Portland |
| Unitary authority |  |
| Shire county |  |
| Region |  |
| Country | England |
| Sovereign state | United Kingdom |
| Post town | Portland |
| Postcode district | DT5 |
| Dialling code | 01305 |
|  | |
| Police | Dorset |
| Fire | Dorset and Wiltshire |
| Ambulance | South Western |
|  | |
| UK Parliament |  |
|


title: Immunoprecipitation

abstract: Immunoprecipitation (IP) is the technique of precipitating a protein antigen out of solution using an antibody that specifically binds to that particular protein. This process can be used to isolate and concentrate a particular protein from a sample containing many thousands of different proteins. Immunoprecipitation requires that the antibody be coupled to a solid substrate at some point in the procedure.

text:
# Immunoprecipitation

**Immunoprecipitation** (**IP**) is the technique of precipitating a protein antigen out of solution using an antibody that specifically binds to that particular protein. This process can be used to isolate and concentrate a particular protein from a sample containing many thousands of different proteins. Immunoprecipitation requires that the antibody be coupled to a solid substrate at some point in the procedure.

## Types

### Individual protein immunoprecipitation (IP)

Involves using an antibody that is specific for a known protein to isolate that particular protein out of a solution containing many different proteins. These solutions will often be in the form of a crude lysate of a plant or animal tissue. Other sample types could be body fluids or other samples of biological origin.

### Protein complex immunoprecipitation (Co-IP)

Immunoprecipitation of intact protein complexes (i.e. antigen along with any proteins or ligands that are bound to it) is known as co-immunoprecipitation (Co-IP). Co-IP works by selecting an antibody that targets a known protein that is believed to be a member of a larger complex of proteins. By targeting this *known* member with an antibody it may become possible to pull the entire protein complex out of solution and thereby identify *unknown* members of the complex.

This works when the proteins involved in the complex bind to each other tightly, making it possible to pull multiple members of the complex out of the solution by latching onto one member with an antibody. This concept of pulling protein complexes out of solution is sometimes referred to as a "pull-down". Co-IP is a powerful technique that is used regularly by molecular biologists to analyze protein–protein interactions.

* A particular antibody often selects for a subpopulation of its target protein that has the epitope exposed, thus failing to identify any proteins in complexes that hide the epitope. This can be seen in that it is rarely possible to precipitate even half of a given protein from a sample with a single antibody, even when a large excess of antibody is used.
* As successive rounds of targeting and immunoprecipitations take place, the number of identified proteins may continue to grow. The identified proteins may not ever exist in a single complex at a given time, but may instead represent a network of proteins interacting with one another at different times for different purposes.
* Repeating the experiment by targeting different members of the protein complex allows the researcher to double-check the result. Each round of pull-downs should result in the recovery of both the original known protein as well as other previously identified members of the complex (and even new additional members). By repeating the immunoprecipitation in this way, the researcher verifies that each identified member of the protein complex was a valid identification. If a particular protein can only be recovered by targeting one of the known members but not by targeting other of the known members then that protein's status as a member of the complex may be subject to question.

### Chromatin immunoprecipitation (ChIP)

Chromatin immunoprecipitation (ChIP) is a method used to determine the location of DNA binding sites on the genome for a particular protein of interest. This technique gives a picture of the protein–DNA interactions that occur inside the nucleus of living cells or tissues. The *in vivo* nature of this method is in contrast to other approaches traditionally employed to answer the same questions.

The principle underpinning this assay is that DNA-binding proteins (including transcription factors and histones) in living cells can be cross-linked to the DNA that they are binding. By using an antibody that is specific to a putative DNA binding protein, one can immunoprecipitate the protein–DNA complex out of cellular lysates. The crosslinking is often accomplished by applying formaldehyde to the cells (or tissue), although it is sometimes advantageous to use a more defined and consistent crosslinker such as dimethyl 3,3′-dithiobispropionimidate-2 HCl (DTBP). Following crosslinking, the cells are lysed and the DNA is broken into pieces 0.2–1.0 kb in length by sonication. At this point the immunoprecipitation is performed resulting in the purification of protein–DNA complexes. The purified protein–DNA complexes are then heated to reverse the formaldehyde cross-linking of the protein and DNA complexes, allowing the DNA to be separated from the proteins. The identity and quantity of the DNA fragments isolated can then be determined by polymerase chain reaction (PCR). The limitation of performing PCR on the isolated fragments is that one must have an idea which genomic region is being targeted in order to generate the correct PCR primers. Sometimes this limitation is circumvented simply by cloning the isolated genomic DNA into a plasmid vector and then using primers that are specific to the cloning region of that vector. Alternatively, when one wants to find where the protein binds on a genome-wide scale, ChIP-sequencing is used and has recently emerged as a standard technology that can localize protein binding sites in a high-throughput, cost-effective fashion, allowing also for the characterization of the cistrome. Previously, DNA microarray was also used (ChIP-on-chip or ChIP-chip).

### RNP immunoprecipitation (RIP and CLIP)

RIP and CLIP both purify a specific RNA-binding protein in order to identify bound RNAs, thereby studying ribonucleoproteins (RNPs). In RIP, the co-purified RNAs are extracted and their enrichment is compared to control, which was originally done by microarray or RT-PCR. In CLIP, cells are UV crosslinked prior to lysis, followed by additional purification steps beyond standard immunoprecipitation, including partial RNA fragmentation, high-salt washing, SDS-PAGE separation and membrane transfer, and identification of direct RNA binding sites by cDNA sequencing.

### Tagged proteins

One of the major technical hurdles with immunoprecipitation is the great difficulty in generating an antibody that specifically targets a single known protein. To get around this obstacle, many groups will engineer **tags** onto either the C- or N- terminal end of the protein of interest. The advantage here is that the same tag can be used time and again on many different proteins and the researcher can use the same antibody each time. The advantages with using tagged proteins are so great that this technique has become commonplace for all types of immunoprecipitation including all of the types of IP detailed above. Examples of tags in use are the green fluorescent protein (GFP) tag, glutathione-S-transferase (GST) tag and the FLAG-tag tag. While the use of a tag to enable pull-downs is convenient, it raises some concerns regarding biological relevance because the tag itself may either obscure native interactions or introduce new and unnatural interactions.

## Methods

The two general methods for immunoprecipitation are the direct capture method and the indirect capture method.

### Direct

Antibodies that are specific for a particular protein (or group of proteins) are immobilized on a solid-phase substrate such as superparamagnetic microbeads or on microscopic agarose (non-magnetic) beads. The beads with bound antibodies are then added to the protein mixture, and the proteins that are targeted by the antibodies are captured onto the beads via the antibodies; in other words, they become immunoprecipitated.

### Indirect

Antibodies that are specific for a particular protein, or a group of proteins, are added directly to the mixture of protein. The antibodies have not been attached to a solid-phase support yet. The antibodies are free to float around the protein mixture and bind their targets. As time passes, beads coated in Protein A/G are added to the mixture of antibody and protein. At this point, the antibodies, which are now bound to their targets, will stick to the beads.

From this point on, the direct and indirect protocols converge because the samples now have the same ingredients. Both methods give the same end-result with the protein or protein complexes bound to the antibodies which themselves are immobilized onto the beads.

### Selection

An indirect approach is sometimes preferred when the concentration of the protein target is low or when the specific affinity of the antibody for the protein is weak. The indirect method is also used when the binding kinetics of the antibody to the protein is slow for a variety of reasons. In most situations, the direct method is the default, and the preferred, choice.

## Technological advances

### Agarose

Historically the solid-phase support for immunoprecipitation used by the majority of scientists has been highly-porous **agarose beads** (also known as agarose resins or slurries). The advantage of this technology is a very high potential binding capacity, as virtually the entire sponge-like structure of the agarose particle (50 to 150 μm in size) is available for binding antibodies (which will in turn bind the target proteins) and the use of standard laboratory equipment for all aspects of the IP protocol without the need for any specialized equipment. The advantage of an extremely high binding capacity must be carefully balanced with the quantity of antibody that the researcher is prepared to use to coat the agarose beads. Because antibodies can be a cost-limiting factor, it is best to calculate backward *from* the amount of protein that needs to be captured (depending upon the analysis to be performed downstream), *to* the amount of antibody that is required to bind that quantity of protein (with a small excess added in order to account for inefficiencies of the system), and back still further *to* the quantity of agarose that is needed to bind that particular quantity of antibody. In cases where antibody saturation is not required, this technology is unmatched in its ability to capture extremely large quantities of captured target proteins. The caveat here is that the *"high capacity advantage"* can become a *"high capacity disadvantage"* that is manifested when the enormous binding capacity of the sepharose/agarose beads is not completely saturated with antibodies. It often happens that the amount of antibody available to the researcher for their immunoprecipitation experiment is less than sufficient to saturate the agarose beads to be used in the immunoprecipitation. In these cases the researcher can end up with agarose particles that are only partially coated with antibodies, and the portion of the binding capacity of the agarose beads that is not coated with antibody is then free to bind anything that will stick, resulting in an elevated background signal due to non-specific binding of lysate components to the beads, which can make data interpretation difficult. While some may argue that for these reasons it is prudent to match the quantity of agarose (in terms of binding capacity) to the quantity of antibody that one wishes to be bound for the immunoprecipitation, a simple way to reduce the issue of non-specific binding to agarose beads and increase specificity is to preclear the lysate, which for any immunoprecipitation is highly recommended.

#### Preclearing

Lysates are complex mixtures of proteins, lipids, carbohydrates and nucleic acids, and one must assume that some amount of non-specific binding to the IP antibody, Protein A/G or the beaded support will occur and negatively affect the detection of the immunoprecipitated target(s). In most cases, *preclearing* the lysate at the start of each immunoprecipitation experiment (see step 2 in the "protocol" section below) is a way to remove potentially reactive components from the cell lysate prior to the immunoprecipitation to prevent the non-specific binding of these components to the IP beads or antibody. The basic preclearing procedure is described below, wherein the lysate is incubated with beads alone, which are then removed and discarded prior to the immunoprecipitation. This approach, though, does not account for non-specific binding to the IP antibody, which can be considerable. Therefore, an alternative method of preclearing is to incubate the protein mixture with exactly the same components that will be used in the immunoprecipitation, except that a non-target, irrelevant antibody of the same antibody subclass as the IP antibody is used instead of the IP antibody itself. This approach attempts to use as close to the exact IP conditions and components as the actual immunoprecipitation to remove any non-specific cell constituent without capturing the target protein (unless, of course, the target protein non-specifically binds to some other IP component, which should be properly controlled for by analyzing the discarded beads used to preclear the lysate). The target protein can then be immunoprecipitated with the reduced risk of non-specific binding interfering with data interpretation.

### Superparamagnetic beads

While the vast majority of immunoprecipitations are performed with agarose beads, the use of superparamagnetic beads for immunoprecipitation is a newer approach that is gaining in popularity as an alternative to agarose beads for IP applications. Unlike agarose, magnetic beads are solid and can be spherical, depending on the type of bead, and antibody binding is limited to the surface of each bead. While these beads do not have the advantage of a porous center to increase the binding capacity, magnetic beads are significantly smaller than agarose beads (1 to 4 μm), and the greater number of magnetic beads per volume than agarose beads collectively gives magnetic beads an effective surface area-to-volume ratio for optimum antibody binding.

Commercially available magnetic beads can be separated based by size uniformity into monodisperse and polydisperse beads. Monodisperse beads, also called microbeads, exhibit exact uniformity, and therefore all beads exhibit identical physical characteristics, including the binding capacity and the level of attraction to magnets. Polydisperse beads, while similar in size to monodisperse beads, show a wide range in size variability (1 to 4 μm) that can influence their binding capacity and magnetic capture. Although both types of beads are commercially available for immunoprecipitation applications, the higher quality monodisperse superparamagnetic beads are more ideal for automatic protocols because of their consistent size, shape and performance. Monodisperse and polydisperse superparamagnetic beads are offered by many companies, including Invitrogen, Thermo Scientific, and Millipore.

### Agarose vs. magnetic beads

Proponents of magnetic beads claim that the beads exhibit a faster rate of protein binding over agarose beads for immunoprecipitation applications, although standard agarose bead-based immunoprecipitations have been performed in 1 hour. Claims have also been made that magnetic beads are better for immunoprecipitating extremely large protein complexes because of the complete lack of an upper size limit for such complexes, although there is no unbiased evidence stating this claim. The nature of magnetic bead technology does result in less sample handling due to the reduced physical stress on samples of magnetic separation versus repeated centrifugation when using agarose, which may contribute greatly to increasing the yield of labile (fragile) protein complexes. Additional factors, though, such as the binding capacity, cost of the reagent, the requirement of extra equipment and the capability to automate IP processes should be considered in the selection of an immunoprecipitation support.

#### Binding capacity

Proponents of both agarose and magnetic beads can argue whether the vast difference in the binding capacities of the two beads favors one particular type of bead. In a bead-to-bead comparison, agarose beads have significantly greater surface area and therefore a greater binding capacity than magnetic beads due to the large bead size and sponge-like structure. But the variable pore size of the agarose causes a potential upper size limit that may affect the binding of extremely large proteins or protein complexes to internal binding sites, and therefore magnetic beads may be better suited for immunoprecipitating large proteins or protein complexes than agarose beads, although there is a lack of independent comparative evidence that proves either case.

Some argue that the significantly greater binding capacity of agarose beads may be a disadvantage because of the larger capacity of non-specific binding. Others may argue for the use of magnetic beads because of the greater quantity of antibody required to saturate the total binding capacity of agarose beads, which would obviously be an economical disadvantage of using agarose. While these arguments are correct outside the context of their practical use, these lines of reasoning ignore two key aspects of the principle of immunoprecipitation that demonstrates that the decision to use agarose or magnetic beads is not simply determined by binding capacity.

First, non-specific binding is not limited to the antibody-binding sites on the immobilized support; any surface of the antibody or component of the immunoprecipitation reaction can bind to nonspecific lysate constituents, and therefore nonspecific binding will still occur even when completely saturated beads are used. This is why it is important to preclear the sample before the immunoprecipitation is performed.

Second, the ability to capture the target protein is directly dependent upon the amount of immobilized antibody used, and therefore, in a side-by-side comparison of agarose and magnetic bead immunoprecipitation, the most protein that either support can capture is limited by the amount of antibody added. So the decision to saturate any type of support depends on the amount of protein required, as described above in the Agarose section of this page.

#### Cost

The price of using either type of support is a key determining factor in using agarose or magnetic beads for immunoprecipitation applications. A typical first-glance calculation on the cost of magnetic beads compared to sepharose beads may make the sepharose beads appear less expensive. But magnetic beads may be competitively priced compared to agarose for analytical-scale immunoprecipitations depending on the IP method used and the volume of beads required per IP reaction.

Using the traditional batch method of immunoprecipitation as listed below, where all components are added to a tube during the IP reaction, the physical handling characteristics of agarose beads necessitate a minimum quantity of beads for each IP experiment (typically in the range of 25 to 50 μl beads per IP). This is because sepharose beads must be concentrated at the bottom of the tube by centrifugation and the supernatant removed after each incubation, wash, etc. This imposes absolute physical limitations on the process, as pellets of agarose beads less than 25 to 50 μl are difficult if not impossible to visually identify at the bottom of the tube. With magnetic beads, there is no minimum quantity of beads required due to magnetic handling, and therefore, depending on the target antigen and IP antibody, it is possible to use considerably less magnetic beads.

Conversely, spin columns may be employed instead of normal microfuge tubes to significantly reduce the amount of agarose beads required per reaction. Spin columns contain a filter that allows all IP components except the beads to flow through using a brief centrifugation and therefore provide a method to use significantly less agarose beads with minimal loss.

#### Equipment

As mentioned above, only standard laboratory equipment is required for the use of agarose beads in immunoprecipitation applications, while high-power magnets are required for magnetic bead-based IP reactions. While the magnetic capture equipment may be cost-prohibitive, the rapid completion of immunoprecipitations using magnetic beads may be a financially beneficial approach when grants are due, because a 30-minute protocol with magnetic beads compared to overnight incubation at 4 °C with agarose beads may result in more data generated in a shorter length of time.

#### Automation

An added benefit of using magnetic beads is that automated immunoprecipitation devices are becoming more readily available. These devices not only reduce the amount of work and time to perform an IP, but they can also be used for high-throughput applications.

#### Summary

While clear benefits of using magnetic beads include the increased reaction speed, more gentle sample handling and the potential for automation, the choice of using agarose or magnetic beads based on the binding capacity of the support medium and the cost of the product may depend on the protein of interest and the IP method used. As with all assays, empirical testing is required to determine which method is optimal for a given application.

## Protocol

### Background

Once the solid substrate bead technology has been chosen, antibodies are coupled to the beads and the antibody-coated-beads can be added to the heterogeneous protein sample (e.g. homogenized tissue). At this point, antibodies that are immobilized to the beads will bind to the proteins that they specifically recognize. Once this has occurred the immunoprecipitation portion of the protocol is actually complete, as the specific proteins of interest are bound to the antibodies that are themselves immobilized to the beads. Separation of the immunocomplexes from the lysate is an extremely important series of steps, because the protein(s) must remain bound to each other (in the case of co-IP) and bound to the antibody during the wash steps to remove non-bound proteins and reduce background.

When working with agarose beads, the beads must be pelleted out of the sample by briefly spinning in a centrifuge with forces between 600–3,000 x g (times the standard gravitational force). This step may be performed in a standard microcentrifuge tube, but for faster separation, greater consistency and higher recoveries, the process is often performed in small spin columns with a pore size that allows liquid, but not agarose beads, to pass through. After centrifugation, the agarose beads will form a very loose fluffy pellet at the bottom of the tube. The supernatant containing contaminants can be carefully removed so as not to disturb the beads. The wash buffer can then be added to the beads and after mixing, the beads are again separated by centrifugation.

With superparamagnetic beads, the sample is placed in a magnetic field so that the beads can collect on the side of the tube. This procedure is generally complete in approximately 30 seconds, and the remaining (unwanted) liquid is pipetted away. Washes are accomplished by resuspending the beads (off the magnet) with the washing solution and then concentrating the beads back on the tube wall (by placing the tube back on the magnet). The washing is generally repeated several times to ensure adequate removal of contaminants. If the superparamagnetic beads are homogeneous in size and the magnet has been designed properly, the beads will concentrate uniformly on the side of the tube and the washing solution can be easily and completely removed.

After washing, the precipitated protein(s) are eluted and analyzed by gel electrophoresis, mass spectrometry, western blotting, or any number of other methods for identifying constituents in the complex. Protocol times for immunoprecipitation vary greatly due to a variety of factors, with protocol times increasing with the number of washes necessary or with the slower reaction kinetics of porous agarose beads.

### Steps

1. Lyse cells and prepare sample for immunoprecipitation.
2. Pre-clear the sample by passing the sample over beads alone or bound to an irrelevant antibody to soak up any proteins that non-specifically bind to the IP components.
3. Incubate solution with antibody against the protein of interest. Antibody can be attached to solid support before this step (direct method) or after this step (indirect method). Continue the incubation to allow antibody-antigen complexes to form.
4. Precipitate the complex of interest, removing it from bulk solution.
5. Wash precipitated complex several times. Spin each time between washes when using agarose beads or place tube on magnet when using superparamagnetic beads and then remove the supernatant. After the final wash, remove as much supernatant as possible.
6. Elute proteins from the solid support using low-pH or SDS sample loading buffer.
7. Analyze complexes or antigens of interest. This can be done in a variety of ways:
    1. SDS-PAGE (sodium dodecyl sulfate-polyacrylamide gel electrophoresis) followed by gel staining.
    2. SDS-PAGE followed by: gel staining, cutting out individual stained protein bands, and sequencing the proteins in the bands by matrix-assisted laser desorption/ionization (MALDI) mass spectrometry.
    3. Transfer and Western blot using another antibody for proteins that were interacting with the antigen, followed by detection using a chemiluminescent or fluorescent secondary antibody.

Co-Immunoprecipitation (Co-IP) Technical


title: Laelaptiella eupodalia

abstract: Laelaptiella eupodalia is a species of mite in the family Ologamasidae.

text:
# Laelaptiella eupodalia

***Laelaptiella eupodalia*** is a species of mite in the family Ologamasidae.

## InfoBox

| *Laelaptiella eupodalia* | |
| --- | --- |
|
| Scientific classification | |
| Domain: | Eukaryota |
| Kingdom: | Animalia |
| Phylum: | Arthropoda |
| Subphylum: | Chelicerata |
| Class: | Arachnida |
| Order: | Mesostigmata |
| Family: | Ologamasidae |
| Genus: | *Laelaptiella* |
| Species: | ***L. eupodalia*** |
| Binomial name | |
| ***Laelaptiella eupodalia***<br>Karg, 1996 | |
|


title: Gustavo Scuseria

abstract: Gustavo E. Scuseria is the Robert A. Welch Professor of Chemistry, Professor of Physics & Astronomy and Professor of Materials Science & NanoEngineering at Rice University, Houston, TX. He is also editor-in-chief of the Journal of Chemical Theory and Computation. Scuseria earned his PhD from the University of Buenos Aires in 1983 and conducted post-doctoral work at the University of California, Berkeley (1985–1987) and the University of Georgia (1987–1989) prior to joining the chemistry faculty at Rice University in 1989.

text:
# Gustavo Scuseria

**Gustavo E. Scuseria** (born July 30, 1956) is the Robert A. Welch Professor of Chemistry, Professor of Physics & Astronomy and Professor of Materials Science & NanoEngineering at Rice University, Houston, TX. He is also editor-in-chief of the *Journal of Chemical Theory and Computation*. Scuseria earned his PhD from the University of Buenos Aires in 1983 and conducted post-doctoral work at the University of California, Berkeley (1985–1987) and the University of Georgia (1987–1989) prior to joining the chemistry faculty at Rice University in 1989.

## Group and research

Since 1989, more than 45 graduate students and 45 post-doctoral researchers have worked in the group, as well as a number of visiting scholars, summer researchers and undergraduate students. Research in the Scuseria group straddles quantum chemistry, condensed matter physics, and materials science, focusing on novel methods for electronic structure theory in molecules and solids, particularly strong correlation, and applications to molecules and materials of importance for energy and the environment. Professor Scuseria has made contributions to density functional theory, coupled cluster theory and the theory of carbon nanomaterials. Recent research thrusts in the group have focused on symmetry breaking and restoration, projected quasiparticle theory, projected Hartree-Fock theory, density embedding methods and pair coupled cluster theory. Scuseria's work has resulted in more than 450 peer-reviewed publications and 350 invited lectures at conferences and institutions all over the world.

## Honors and awards

Scuseria's work has been recognized by a number of awards. These include the Oak Ridge Associated Universities Junior Faculty Award, the Camille and Henry Dreyfus Teacher-Scholar Award, an IBM Partnership Award, a John Simon Guggenheim Memorial Foundation Fellowship, a Creativity Extension Award from the Chemistry Division of the National Science Foundation, and the Feynman Prize in Nanotechnology Theory. Scuseria is a Fellow of the American Association for the Advancement of Science, the American Physical Society, the Royal Society of Chemistry, the American Chemical Society, and he is an elected member of the International Academy of Quantum Molecular Science, of which he has been vice president since 2012. In 2016, Scuseria was listed as an ISI highly cited researcher.

## InfoBox

| Gustavo E. Scuseria | |
| --- | --- |
| Website | http://scuseria.rice.edu/ |


title: Haitoku no Scenario

abstract: "Haitoku no Scenario" is the 12th single by Japanese idol duo Wink. Written by Neko Oikawa and Takashi Kudō, the single was released on October 16, 1991, by Polystar Records.

text:
# Haitoku no Scenario

"**Haitoku no Scenario**" (背徳のシナリオ, *Haitoku no Shinario*, lit. "An Immoral Scenario") is the 12th single by Japanese idol duo Wink. Written by Neko Oikawa and Takashi Kudō, the single was released on October 16, 1991, by Polystar Records.

## Background and release

"Haitoku no Scenario" was written and composed as a fusion of Russian traditional music and 1970s disco, themed around a lonely man living in a cold Siberian town. Like the duo's previous single "Manatsu no Tremolo", the song was used by Panasonic for their RQ-S35 and RQ-S35V headphone commercial. The B-side, "Jūnigatsu no Orihime", is a Japanese-language cover of Zoe Haywood's "My World".

"Haitoku no Scenario" peaked at No. 3 on the Oricon's weekly charts and sold over 196,000 copies.

## Track listing

All lyrics are written by Neko Oikawa; all music is arranged by Satoshi Kadokura.

| No. | Title | Music | Length |
| --- | --- | --- | --- |
| 1. | "Haitoku no Scenario" (*Haitoku no Shinario* (背徳のシナリオ, "An Immoral Scenario")) | Takashi Kudō | 4:27 |
| 2. | "Jūnigatsu no Orihime" ((12月の織姫, "Orihime in December")) |  | 4:46 |

## Chart positions

Weekly charts

| Charts (1991) | Peak
position |
| --- | --- |
| Japanese Oricon Singles Chart | 3 |

Year-end charts

| Charts (1991) | Peak
position |
| --- | --- |
| Japanese Oricon Singles Chart | 108 |

## InfoBox

| "Haitoku no Scenario" | |
| --- | --- |
|  | |
| Single by Wink | |
| from the album  *Sapphire* | |
| Language | Japanese |
| English title | An Immoral Scenario |
| B-side | "Jūnigatsu no Orihime" |
| Released | October 16, 1991 (1991-10-16) |
| Recorded | 1991 |
| Genre |  |
| Length | 4:27 |
| Label | Polystar |
| Composer(s) | Takashi Kudō |
| Lyricist(s) | Neko Oikawa |
| Producer(s) | Haruo Mizuhashi |
| Wink singles chronology | |
| "Manatsu no Tremolo"
(1991) "**Haitoku no Scenario**"
(1991) "Tsuioku no Heroine"
(1991) | |
|  | |
| Music video | |
| "Haitoku no Scenario" on YouTube | |
|  | |


title: KDSJ

abstract:
KDSJ is a radio station broadcasting a full-service format. Licensed to Deadwood, South Dakota, United States, the station serves the Black Hills area. The station is currently owned by Carolyn and Doyle Becker, through licensee Riverfront Broadcasting, LLC. The station has had several studio locations since its debut in 1947. In 1952, the station moved to its present location, a former funeral home, at 745 Main Street in Deadwood. Its two tower transmitter site is in Boulder Canyon on Radio Tower Road. The station broadcasts with 5,000 watts during the day and 1,000 watts at night.
Effective June 30, 2020, the station was purchased for $80,000 by Riverfront Broadcasting from 4 Paws Broadcasting. Riverfront owns four FM stations in Rapid City and one AM station. As part of this, the station has announced it will be moving to Lead, hopefully completed by Fall of 2021.

text:
# KDSJ

**KDSJ** (980 AM) is a radio station broadcasting a full-service format. Licensed to Deadwood, South Dakota, United States, the station serves the Black Hills area. The station is currently owned by Carolyn and Doyle Becker, through licensee Riverfront Broadcasting, LLC.

The station has had several studio locations since its debut in 1947. In 1952, the station moved to its present location, a former funeral home, at 745 Main Street in Deadwood. Its two tower transmitter site is in Boulder Canyon on Radio Tower Road. The station broadcasts with 5,000 watts during the day and 1,000 watts at night. Effective June 30, 2020, the station was purchased for $80,000 by Riverfront Broadcasting from 4 Paws Broadcasting. Riverfront owns four FM stations in Rapid City and one AM station. As part of this, the station has announced it will be moving to Lead, hopefully completed by Fall of 2021.

## InfoBox

KDSJ
|  | |
| --- | --- |
|  | |
| Broadcast area | Black Hills Area |
| Frequency | 980 kHz |
| Branding | Live. Local. News. Weather. Sports. |
| Programming | |
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| Ownership | |
| Owner | * Carolyn and Doyle Becker * (Riverfront Broadcasting, LLC) |
| History | |
| First air date | August 1947 |
| Technical information | |
| Licensing authority | FCC |
| Facility ID | 24553 |
| Class | B |
| Power | 5,000 watts day<br>1,000 watts night |
| Transmitter coordinates | 44°22′57″N 103°39′44″W / 44.38250°N 103.66222°W / 44.38250; -103.66222 |
| Translator(s) | 103.5 K278AM (Spearfish) |
| Links | |
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| Webcast | Listen Live |
| Website | kdsj980.com |


title: Hanky panky (cocktail)

abstract: The hanky panky is a cocktail made from gin, sweet vermouth, and Fernet-Branca. It is a variation on the sweet martini, or Martinez, made distinctive by the Fernet-Branca, a bitter Italian digestivo. It was created by Ada "Coley" Coleman, head bartender at the Savoy Hotel, London.

text:
# Hanky panky (cocktail)

The **hanky panky** is a cocktail made from gin, sweet vermouth, and Fernet-Branca. It is a variation on the sweet martini, or Martinez, made distinctive by the Fernet-Branca, a bitter Italian digestivo. It was created by Ada "Coley" Coleman, head bartender at the Savoy Hotel, London.

## Recipe

A recipe for the cocktail was included in Harry Craddock's *The Savoy Cocktail Book*.

*In a cocktail shaker over ice pour:* * 12 (1+12 oz) Italian vermouth
* 12 (1+12 oz) dry gin
* 2 dashes Fernet-Branca

*Stir* *Strain into a (4 oz.) cocktail glass.* *Garnish by squeezing an orange peel over the top.*

## History

The hanky panky was the brainchild of Ada Coleman (known as "Coley") who began as a bartender at the Savoy Hotel in 1903. Her benefactor was Rupert D'Oyly Carte, a member of the family that first produced Gilbert and Sullivan operas in London and that built the Savoy Hotel. When Rupert became chairman of the Savoy, Ada was given a position at the hotel's American Bar, where she eventually became the head bartender and made cocktails for the likes of Mark Twain, the Prince of Wales, Prince Wilhelm of Sweden, and Sir Charles Hawtrey.

Coleman created the hanky panky for Hawtrey. He was a Victorian and Edwardian actor who mentored Noël Coward. Coleman told the story behind the creation of the hanky panky to England's *The People* newspaper in 1925:

> The late Charles Hawtrey ... was one of the best judges of cocktails that I knew. Some years ago, when he was overworking, he used to come into the bar and say, "Coley, I am tired. Give me something with a bit of punch in it." It was for him that I spent hours experimenting until I had invented a new cocktail. The next time he came in, I told him I had a new drink for him. He sipped it, and, draining the glass, he said, "By Jove! That is the real hanky-panky!", And Hanky-Panky it has been called ever since.

The hanky panky is a variation on the sweet martini, inasmuch as it calls for gin and sweet vermouth, but Coley's secret ingredient that made the drink distinctive was Fernet-Branca, a bitter Italian digestivo.

## InfoBox

Hanky panky
| IBA official cocktail | |
| --- | --- |
|  | |
| Type | Mixed drink |
| Base spirit |  |
| Served | Straight up: chilled, without ice |
| Standard garnish | Orange zest |
| Standard drinkware | Cocktail glass |
| IBA specified<br>ingredients† | * 45 ml London dry gin * 45 ml Sweet red vermouth * 7.5 ml Fernet-Branca |
| Preparation | Stirred over ice, strained into a chilled glass, garnished, and served up. |
| Hanky panky recipe at International Bartenders Association | |


title: Gerald Rygate

abstract: Gerald Blake Rygate was an Australian politician. Born at Grenfell to medical doctor Robert Edward Rygate, he was educated at St Ignatius College, Riverview. He also studied medicine subsequently joining the Irrigation Commission as a clerk in Leeton, NSW. Granted extended leave Rygate enlisted in the AIF in August 1915. He served in Egypt and France, was wounded in action and was twice mentioned in despatches. On his return he farmed at Canowindra. On 7 February 1923 he married Amelia Rice, with whom he had a son. A Labor Party member since 1912, he was prominent in local politics and sports administration, served as coroner, and actively promoted returned servicemen's welfare causes. He was elected to the New South Wales Legislative Council in 1952. In 1959 he was expelled from the party for voting against the abolition of the Legislative Council, and became associated with the Independent Labor Group. In failing health, his attendance in the Council diminished in 1960 and he died in June at Waverley. His widow Amelia entered the Legislative Council in 1961.

text:
# Gerald Rygate

**Gerald Blake Rygate** (24 February 1895  8 June 1960) was an Australian politician.

Born at Grenfell to medical doctor Robert Edward Rygate, he was educated at St Ignatius College, Riverview. He also studied medicine subsequently joining the Irrigation Commission as a clerk in Leeton, NSW. Granted extended leave Rygate enlisted in the AIF in August 1915. He served in Egypt and France, was wounded in action and was twice mentioned in despatches.

On his return he farmed at Canowindra. On 7 February 1923 he married Amelia Rice, with whom he had a son.

A Labor Party member since 1912, he was prominent in local politics and sports administration, served as coroner, and actively promoted returned servicemen's welfare causes.

He was elected to the New South Wales Legislative Council in 1952. In 1959 he was expelled from the party for voting against the abolition of the Legislative Council, and became associated with the Independent Labor Group. In failing health, his attendance in the Council diminished in 1960 and he died in June at Waverley. His widow Amelia entered the Legislative Council in 1961.


title: Golisano Children's Museum of Naples

abstract: The Golisano Children's Museum of Naples (C'MON) is a children's museum in Naples, Florida, United States C'MON was founded in 2002 and is a 501(c)(3) not-for-profit charitable organization and a member of the Association of Children's Museums. The building is two stories and 30,000 square feet. C'MON "invites visitors of all ages to journey through the swamps of the Everglades, weave through a maze, climb a two-story banyan tree, or experiment with the water play station."

text:
# Golisano Children's Museum of Naples

The **Golisano Children's Museum of Naples** (**C'MON**) is a children's museum in Naples, Florida, United States

C'MON was founded in 2002 and is a 501(c)(3) not-for-profit charitable organization and a member of the Association of Children's Museums. The building is two stories and 30,000 square feet.

C'MON "invites visitors of all ages to journey through the swamps of the Everglades, weave through a maze, climb a two-story banyan tree, or experiment with the water play station."

## WGCU Presents

Every month the museum presents a show from PBS for an event.

26°15′58″N 81°45′07″W / 26.26598°N 81.75207°W / 26.26598; -81.75207