suppose you perfrom a graim stain on a sample from a pure culture of bacteria and observed a field of purple?
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That someone's biology homework is due in tomorrow?
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Burn it in a sprectrophotometer.  Look at the emitted light.
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macroscopic. you "farm out" the bacteria on a agar plate, then describe what grows (usually after 48 hours, but can both be more or less- depending on the species)\nNote: there are VERY many possible culture media (usually Agar based), and some bacteria will grow only on a few of them- that helps with the ID\nAlso- some bacteria need special culture conditions- eg. anaerobes- which die if they are cultured in contact with air. Also helps with the ID.\n\nMicroscopic. First of all look at the bacteria under a microscope- that gives you some info.- eg are they round (cocci) or elongated (bacili) or curved (vibrio) or spiral, or any of a multitude of shapes.\nsome bacteria are transparent and can only be seen in a "dark field" (reflected light from bacteria seen on an otherwise unlighted space) typical for Treponema pallidum- causes syphilis.\n\nThen the bacteria can be stained- very many possible stains and methods- but the most commonly known is the Gram stain (hence some bacteria are called "Gram positive" or Gram negative" - depending if the stain "caught" on the cell wall.\nSome stains are then removed with acid preparations- and in some bacteria it works, in others not. eg. the TB bacteria are known as "acid fast"- meaning an acid bath does not remove the colour of the original stain\n\nEssentially you are supposed to go through all the steps and look up in a book what fits your particular bug.\nThe bug can also be a fungus- yeast or mould
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Please visit:\nhttp://www.homeofpoi.com/articles/colouredflames.php
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Here's an interesting experiment.
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Gram staining is a laboratory test used for identifying various kinds of bacteria.  Some kinds of bacteria react to the stain and those are gram positive, the rest don't and they are gram negative.  Different kinds of antibiotics are designed to kill bacteria that are gram positive, gram negative or both.
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A pyogenic infection just means it's an infection producing pus. So, to diagnose any infection, you could take out the pus and send it to the laboratory for culture to see what organisms grow during the culture. In addition, if for example, the doctor thinks it could be a kidney infection, he/she could send a clean urine specimen for culture.  If the doctor thinks the bacteria can be found in the bloodstream, he/she could take some blood from you and send it off for culture. Then there are other investigations, for example, a chest x-ray where one can see some shadow in the chest x-ray which would suggest a chest infection. And so on.
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Cells are colorless until they are stained. They color that they appear is then based on what type of stain was used. Hope that helps.
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No.  Proteus vulgaris is not one of the acid fast organisms.  It'll stain just fine with Gram's stain.\n(Gram Negative rod)
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No.  Pseudomonas fluorescens is a species of bacteria.  It is not an acid fast bacteria.\nAcid fast bacteria include Mycobacteria, certain Nocardia sp. and some yeasts such as Cryptosporidium.  \nThe acid fast stains are: Kinyoun and Ziehl-Neelson.\nMost acid fast bacteia also stain with the auramine-rhodamine stain which is a fluorescent stain.
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You can also perform classical staining according to Gram, B. subtilis is G+ rod, than you inoculate meat-extract agar plate, incubate it for several days and old culture you can check for endospores microscopically after staining according to Wirtz and Couhlin. Spores are typically located.\nSome experiences are needed to identificate Bacilli this way.
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You could use a turbidometric method that could detect the diffraction of light through the liquid and correlate it with the amount of microbial growth in it.  \n\nYou could also use a calibrated loop to plate the liquid and then count colonies.  I know they do this with urine cultures in hospital labs at times.  The loop picks up a certain amount of liquid like 10 microliters or so and then you can correlate the growth with this 10 microliter figure.
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You can either look it up or determine it experimentally.\nI cant get much more specific without additional details.
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there r 2 kinds of gram-stain..positive n negative...use 2 kinds of reaction..the first is iodium n use alcohol as the cleaner..n the positive gram will keep the color of iodium so it will stay b purple...n use the second reaction which is safranin or methylene blue then the negative gram will be red or blue...
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It's the cell wall. The peptidoglycan in the gram positive bacterial cell wall retains the crystal violet and make them look purple in color.
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What class was this in? What grade level? What text were you using?  I may be able to help you if I know the context in which the question was raised. I have a feeling that I am not getting all the information you had. Please tell me and maybe I can help. I am an old chemist and microbiologist.
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I THINK IT WAS PURPLE.