What are some sterile technique I would need to know?
916415
Firstly, is the culture tube to be a nutrient broth or a nutrient agar?\n\nWhichever it is, both will have to be autoclaved to remove all other organisms. After autoclaving, don't open until you want to insert the sample. Make sure you have a naked flame in front of you. The area just around the flame is your sterile area. \n\nFor a nutrient agar, heat your innoculating loop in the flame until it is red-hot then remove from the flame and flick it to cool it. Test on one side of the agar to make sure it is not too hot. If too hot, the agar will hiss. Collect your sample of E. coli with the innoculating loop and use your other hand to hold the culture tube. Open the culture tube with as few fingers as possible. Streak the E. coli on the nutrient agar making sure to spread it evenly. Always keep the mouth of the tube near the flame. The innoculating loop should also always be near the flame until the process is complete. Close the culture tube and heat the innoculating loop again.\n\nIf it is nutrient broth, then follow the same steps as above except that you insert the innoculating loop into the broth and shake it. Then close as above.\n\n*Remember... when opening the tubes, always keep the mouth of the tube close to the flame and always sterilise the innoculating loops.*\n\nEDIT: Answer to the replied question.\n\nYeah a micropipette would be easier. You'll need to autoclave the micropipette tips before using and like I said, all the procedures have to be carried out right beside the flame. The mouth of the culture tube has to be in or right beside the flame so that no contamination happens.\n\nIn any case, the innoculating loop or the pipette can be used because the nutrient broth is there for the bacteria to develop colonies and grow so the end result will still be the same.
1174597
Take a (Warm) bath with Epsom salts\nDON'T USE A SOLDERING GUN!!!\nUSE Preparation H instead.\n\nGO TO The hospitals ER room or a MEDICAL CLINIC!!!
903880
get the doctor to check it out hun - best way to avoid infection :)
214979
You can check out this site, it explains a step by step technique:\n\nhttp://www.pall.com/biosepra/34696_7290.asp
655620
Just get a pool test kit.  It will give you bacterial levels to determine if it's safe to spa.
160725
Prick it with sterilized needle.
76056
take your soil sample and suspend some in a little sterile water; plate out the suspension on agar plates in the usual way and isolate the colonies as a way to do a preliminary sort. Then maybe culture them ... Then do your in-depth analysis.
213388
Pot of soil: Put all the soil on a baking sheet and put it in the very hot oven until its heated all through and then another couple of minutes. Or freeze it for a couple of days in the freezer (this won't sterilize it but it would get rid of flies etc. - insect eggs wouldn't survive that. Bacteria can.).\nInside of a fridge: Sterilisation of such a big thing in a home is not possible. You can reduce the amount of germs tremendously if you wash it out with concentrated bleach/desinfectant etc. on a regular basis. \nFruit in plastic bags: Sterilisation not possible in original form. Wash with soap (works fine for apples etc.) and keep in fridge to reduce germ increase. Or if you really need sterile, make preserves - wash, peel and preserve them in steam in cans or glass.
562813
Your last question talked about Diabetes.  Are You talking about being pricked by a needle of someone who has diabetes?  If so that can not be transmitted.  If You or someone You know has actually been pricked, you need to try and push out as much blood as you can to release all the possible substance,  then clean with hydrogen peroxide like Someone else suggested.  Then You need to have blood tests done, if it is a shared needle, a used needle that You have No idea Who used it, or especially if it is Someone with Hepatitis C, HIV, or Aids.
106065
Yes. There are a lot of other ways like Using infected needles, etc.\nYou can find a lot of info at http://www.thebody.com/
108656
Well, since 80% of all infections are spread through direct contact, I'd suggest that you get in the habit of washing your hands frequently and well.  Other than that, maintain a healthy weight through a good diet and exercise; stay away from behaviors clearly linked to increased risk (like smoking), etc.
1037651
Needle and Thread work wonders.\n\n\n\ndp
1045410
First you'll need:\nTwo tablespoons of mouthwash\nThree shoe laces\nA pinch of butt hair\nAnd a match\n\nI'll let you take it from there.
1032923
minimal infection. dont worry wash &disinfect it by betadine or alcohol.
434999
Get a wet cotton swab and get it out that way. If you use alcohol, you can clean it out at the same time. I like this question! You're funny!
567354
You can get disposable, sterile swabs.  These would probably work the best.  You should be able to get them via online... google "lab equipment swabs".\n\nopen your swab, being careful to not touch the tip.  Run the swab over the section you want to culture.  Then, simply smear the swab onto the petri dish.  \n\nFor the petri dishes, you probably want more than just an empty dish.  You want an agar plate.  An agar plate is a gelatinous material on the bottom of the dish that provides food and nourishment for the bacteria.\n\nYou could probably use gelatin as a stand-in.  Buy plain gelatin and make a batch twice as concentrated as it should be.  This will make the gelatin harder and less likely to melt at room temperature.\n\nGood luck!
171384
bleach\namonia(dont mix with bleach)\nAlcohol(pocibly boose and beer)\nAnti bacterial soap\nSpit/dog slober(ensymes)\nurin(amonia(eewww nasty))\nhot water\nsomething involving a microvave toster or some other heating apliance\nsalt water\nor you could shoot them(aim carefully, bacteria is small)