I NEED HELP WITH MY pGLO TRANSFORMATION LAB FOR A.P. BIO?
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Ask your teacher, I have no idea what that is.
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Rather than microbio, bio, or botany departments I would take it to the plant pathology department...good luck
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good luck: http://www.emc.maricopa.edu/faculty/farabee/BIOBK/BioBookPLANTANAT.html
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http://lab.lpicn.org/pub/books/
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Call Meriter Hospital 267-6000. Maybe they do it, or have connections to a lab that does.
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You can check out this site, it explains a step by step technique:\n\nhttp://www.pall.com/biosepra/34696_7290.asp
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Try browsing this site\n\nhttp://www.elfqrin.com/docs/biospw.html
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Good news: You won't need to read a whole book to get even losts of details as well as the basics: google for a "polymerase chain reaction faq" on the web. Its less complicated than you think. \n\n\nWant to spend less time commuting ? Check out http://www.hallisystem.bravehost.com  \nWrite us.
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http://www.whybiotech.com/index.asp?id=4515
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This website should help out.\nwww.freeessays.cc/db/39/pko247.shtml
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Check out the buffer recipe calculator at <http://www.bioinformatics.org/JaMBW//5/4/index.html>\n\nSome things to consider:\n\nAt what temperature do you want the pH to be 7.5? (the pKa of tris-base varies as a function of T)\n\nWhat ionic strength do you need?
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I assume you know what osmosis is.\nIt would be nice to end up with enough results to plot a graph (LINE graph) so you will need at least four (more is better) solutions of different concentrations. Sucrose solution is usually used.\nUse a BIG potato so that all your samples are as similar as possible.\nThe easiest way to get uniform samples is to cut cylinders with a cork borer, then cut into 5mm disks.\nPut 10 discs into each solution.\nWeigh 10 discs before they are put in, then CAREFULLY blot them dry and reweigh them next day.\nCalculate the change in mass per gram of potato (you could expect some to gain, some to stay the same and some to lose so you will get some negative values).\nPlot a line graph of 'change in mass per gram of potato' AGAINST 'concentration of solution'.\nNow you must consider what the graph show you.
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You should go to the local college bookstore and get the introductory bio textbook.  THere are tons of them out there, I don't know of any one that is better than the others.  It would help if you had a syllibus so you know what subjects will be discussed.  I wouldn't worry about it until you take the course though, you'll probably forget everything you've read if you just read the book straight through.
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Here you go...\n\nhttp://biog-101-104.bio.cornell.edu/Biog101_104/tutorials/frog.html\nhttp://www.infovisual.info/02/026_en.html
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try this:\nhttp://eny3005.ifas.ufl.edu/lab1/
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Yo man i shall give u a few sites that wil enable u to design the animal cell and then fill in the design with the items u believe will suit.\n\nhttp://www.batesville.k12.in.us/bhs/science/paper_bag_cell_model.htm\n\nhttp://www.batesville.k12.in.us/bhs/science/sponge_cell_model.htm\n\nhttp://www.batesville.k12.in.us/bhs/science/styrofoam_cell_model.htm\n\nhttp://www.cellsalive.com/cells/animcell.htm\n\n\n\n\n\nfurther details: mng_1432@2die4.com
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You are not very specific about what you want to do, do you have a sequence you want to translate? Or are you looking for different open reading frames in a sequence? Or are you looking at sequence homology.\n\nI'm a big fan of the ExPASY site, it has lots of links and so on. You can do your BLAST searches to look for sequence homologies, use the translate and reverse translate tools for looking at different open reading frames and it has all the proteomics databases on there as well.\n\nhttp://tw.expasy.org/\n\nI've also added the site for the NCBI BLAST search.\n\nhttp://www.ncbi.nlm.nih.gov/BLAST/