what is the general procedure for designing a microbial medium?
917220
Beyond a sterile petri dish, it would all depend on which microbe you were wanting to cultivate.
910037
A good example is brewing beer.\nAny process usually fermentation that uses living organisms to convert one substance (like sucrose) to another valuable chemical. For sucrose that would be alcohol and carbon dioxide.\nMany other antibiotic production requires fermentation (bio processing), in large vats with specific bacteria or fungi.\nHuman growth hormone is made in fermenters using genetically engineered bacteria.\nOther less sophisticated bio process is the production of linen fiber from the flax plant. The lignin is broken down by bacterial rotting in water like streams etc.The freed fiber is then collected and turned into yarn. Coffee seeds are rotted in order to get the freed beans. They are left to "ot" in the sun for about a weak. Chocolate beans also require bio processing.\nDoc. Dan.
270909
Yo man i shall give u a few sites that wil enable u to design the animal cell and then fill in the design with the items u believe will suit.\n\nhttp://www.batesville.k12.in.us/bhs/science/paper_bag_cell_model.htm\n\nhttp://www.batesville.k12.in.us/bhs/science/sponge_cell_model.htm\n\nhttp://www.batesville.k12.in.us/bhs/science/styrofoam_cell_model.htm\n\nhttp://www.cellsalive.com/cells/animcell.htm\n\n\n\n\n\nfurther details: mng_1432@2die4.com
100001
First you have to determine the bacteria strain.
916415
Firstly, is the culture tube to be a nutrient broth or a nutrient agar?\n\nWhichever it is, both will have to be autoclaved to remove all other organisms. After autoclaving, don't open until you want to insert the sample. Make sure you have a naked flame in front of you. The area just around the flame is your sterile area. \n\nFor a nutrient agar, heat your innoculating loop in the flame until it is red-hot then remove from the flame and flick it to cool it. Test on one side of the agar to make sure it is not too hot. If too hot, the agar will hiss. Collect your sample of E. coli with the innoculating loop and use your other hand to hold the culture tube. Open the culture tube with as few fingers as possible. Streak the E. coli on the nutrient agar making sure to spread it evenly. Always keep the mouth of the tube near the flame. The innoculating loop should also always be near the flame until the process is complete. Close the culture tube and heat the innoculating loop again.\n\nIf it is nutrient broth, then follow the same steps as above except that you insert the innoculating loop into the broth and shake it. Then close as above.\n\n*Remember... when opening the tubes, always keep the mouth of the tube close to the flame and always sterilise the innoculating loops.*\n\nEDIT: Answer to the replied question.\n\nYeah a micropipette would be easier. You'll need to autoclave the micropipette tips before using and like I said, all the procedures have to be carried out right beside the flame. The mouth of the culture tube has to be in or right beside the flame so that no contamination happens.\n\nIn any case, the innoculating loop or the pipette can be used because the nutrient broth is there for the bacteria to develop colonies and grow so the end result will still be the same.
269855
Its a good medium to grow bacteria and allows them to clearly be seen. Also,its relatively inexpensive to make agar.
136153
HI\nYou should be able to make your own media at home.  Make sure that you keep\nthe petri dishes in their covering until just before you use them or they\ncan become contaminated.  You are right, the bouillon cubes won't "melt".\nYou can get some Knox brand gelatin from the store and make it kind of like\nyou would make jello for Finger Jell-O other words you want it a little\nstiffer than normal.  Follow the directions on the package but add perhaps a\nlittle less water.  Once you have boiled the water and added the gelatin, add\nsome beef bouillon to it and dissolve it.  This give the nutrients that the\nbacteria need.  Keep in mind, that this may not completely sterilize the\nmedia-there are bacteria that can survive boiling temperatures.  Pour a\nlittle into a petri dish and let it solidify.  This may take some trial and\nerror to get it the consistency that you want.  I am also thinking that if\nyou are testing the effect of boiling the water you may want to add a little\nof your specimen directly to the agar before it solidifies.  Then any\ncolonies which might be in the water specimen will be mixed directly in with\nthe agar.  You would then count the colonies after incubating.  You can\nincubate them at room temperature for 48-72 hours.  Since you are not\ncompletely sterilizing the agar itself you may be confused by any colonies\nthat grow-do they come from the agar itself or from the boiled water?  Unless\nyou can autoclave your media, it will be difficult to completely sterilize\nyour media.
924623
If you mean how do you culture, pure cultures of bacteria, then one of the easiest ways is to get a culture plate of generic media (broad appeal media, not specific to certain bacteria types), clean a nichrome wire in a flame, dip it into whatever has bacteria you wanna try and culture (old mayonnaise, rotten meat, lake water), streak (run it back and forth along one side of the media),  clean the wire in the flame, alter the media by 90 degs, streak again, making sure the wire passes into the area previously streaked a few times, repeat one more time, place the lid on the plate, turn upside down and place in a incubator for 24 to 48 hours. Pure cultures (those grown from a single bacterium) will have grown up in the second and third streaking areas.
745174
E coli loves a basin or the floor or basically anything that has food for it. I suggest you go to a store that sells scientific equipment and buy a petri dish with agar gel in it. Agar gel is a substance that enables bacteria to grow on it. You should take your sample and gently rub it on the agar gel, but be sure that you do not let the dish be exposed too long to the air, because bacteria is everywhere and you might get some unwanted cultures on your dish.\nYou must then keep the dish at room temperature for at least three days. \nThe E.coli will be visible on the agar gel, but for best viewing of the culture, a microscope is needed, because without it you will only see a yellow line where you rubbed the sample.\nA microscope is a scientists best friend, because with that we can see worlds that other people did not even know existed. you definitely need a microscope.
141967
The well in the chamber is to contain the buffer solution. The well in the agarose is to contain the sample.\n is that what you needed??
1140853
One of the best current techniques is called Design of Experiments (DOE).  There are numerous software packages to assist in proper design and give you the results.  The design consists of determining the number of variables and the levels of each variable you want to test.  You must also decide how many samples of each variable set you want to check.  For the first experiment keep the number of variables and the number of levels of each variable to a minimum.\n\nFor example:  If you want to determine the best way bake a pizza,  you may select oven temperature, bake time, and position in the oven.  If you decided to test two levels of each of these variables ( 400 & 450 degrees, 12 & 15 minutes, lower and upper rack position). For a full experiment, you would have to bake 8 pizzas.  If you wanted to bake 2 of each combination, to gain confidence in the results, you would have to bake 16 pizzas.\n\nIf you wanted to test three levels of each variable, the number would jump to 27 pizzas and 54 pizzas, which would be vey costly.  Keep the experiment simple and use the same brand/type pizza for each combination.\n\nYou may get great results with more than one combination, that's good.  If you get the same results with many combinations, you will have to redesign your experiment to widen the levels or pick a different variable to test.\n\nHope this helps.
232626
Several companies make packs that generate CO2 and N once opened.  They also bind atmospheric oxygen (O2).  If you plate your specimen on an anaerobic media, then place the media in an airtight container with one of these packs, you should acheive the appropriate environment. (You can use an anaerobic indicator to make sure that you've acheived the right air mix.)\n\nWhile plating the organisms, be sure to be quick.  Some organisms are killed by O2, so the longer it takes you to plate them, the more chance there is for O2 to get in and damage them.
151470
depends on what classes of microbe you are reffering to: aerobic (needs oxygen) or anaerobic (oxygen is poison to them). Most all microbes love molasses and high starch items like rice. These substrates offers a wide spectrum of minerals and sugars. The minerals provide "enzyme bridges" for different kinds of microbes to exist "happily" together (even aerobes and anaerobes).
363827
Depends on what you do or care about.  Do you want to know how much sugar to add when making wine to reach the desired alcohol content and/or "dryness"?  Do you want to know how much acetylene and oxygen to mix when welding?  Do you want to fill a scuba diving tank?  Do you want to know how much vinegar and baking soda to add when making sponge toffee so it is neither bitter nor sour?  \n\nThese are just a few examples for people who are not scientists at all.  Zillions of reasons more.
130049
Find a petri dish and some bacteria culture.\n\nSwab your mouth.\n\nRub it across the culture.\n\nLet it sit in the incubator for 24 hours.\n\nAnd voila!\n\nAll the microogranisms in your mouth.
794714
can do the following:\n\n1. Add microbial agents to antimicrobial agents\n\n2. Add Macrobial Agents\n\n3. Add Antimicro-unial agents
942912
To make vinegar the simplest way you need to find yourself; \n\nA container with a spout .(e.g. a sun tea jar) The spout is not mandatory but it sure makes things easier. The container should also have a wide mouth to let in air as well as a way to keep out flies. (Air is very important!) You will be visited by vinegar flies!  They are my assistants. The container should be glass or stainless steel for best results. Aluminum and iron is definately out.  Some plastics can work, some are dangerous because they react with vinegar. So, for now, I would skip plastics. \n\nSome fresh fruit juice. (Even the frozen variey will do. But I would stay away from the bottled ones because they add chemicals to keep the juice from turning to vinegar. (See how easy it is to make vinegar.) \n\nA starter culture. Notice I said "starter culture".  Don't make a big deal about getting a "mother", it will probably ruin otherwise good vinegar. What you need are the bacteria which make vinegar.  Check the home brew stores or pick up a bottle of unpasturized, unfiltered vinegar.  I have had great success with Braggs Apple cider vinegar. The vinegar in the culture keeps out the other molds and bacteria until the vinegar bacteria have had a chance to take firm control of the juice. \n\nA dark place.  You could also paint your jar or cover it . The object is to keep out the light. Light will slow the vinegar production or even kill your culture. \n\nA warm place. The precise temperature is not so critical but it does make a difference on how fast your vinegar is made. If you feel comfortable at that temperature, most likely the vinegar bacteria will be happy also. \n\nOK, we have a vinegar culture, a container to put it in, some food for it and lot's of warm air available to it.\n\nPour about one quart of the starter into the container. \n\nPour about the same amount of juice into the container. \n\nPut the mix into a warm dark place. \n\nKeep checking it until it is as strong as you like it or it seems to be losing strength. \n\nBottle it in small bottles. \n\nLeave it for at least six months before using.  (You could use it right away but, this will make it smoother) \n\nOnce you have got the hang of it, you might want to try making some real special vinegar.  \n\nAnd Remember the Vinegar Man loves you.