how do you culture bacteria?
924623
If you mean how do you culture, pure cultures of bacteria, then one of the easiest ways is to get a culture plate of generic media (broad appeal media, not specific to certain bacteria types), clean a nichrome wire in a flame, dip it into whatever has bacteria you wanna try and culture (old mayonnaise, rotten meat, lake water), streak (run it back and forth along one side of the media),  clean the wire in the flame, alter the media by 90 degs, streak again, making sure the wire passes into the area previously streaked a few times, repeat one more time, place the lid on the plate, turn upside down and place in a incubator for 24 to 48 hours. Pure cultures (those grown from a single bacterium) will have grown up in the second and third streaking areas.
874146
lower temperatures help inibit the proliferation of pathogens (bacteria)
816577
I generally follow the recommendations provided by our safety officer at my academic lab.\n\nIf your liquid cultures can afford the extra volume, you can just add a lot of deionized h2o, that should stop the growing (by lysing the cells).  Then toss in the bio-hazard bag, for the autoclave.  \n\nOr, if you have access to an autoclave, then autoclave the culture, and you can probably dump down the drain.\n\nCheck with your safety officer!!\n\nIt was fun thinking about your question.  Pass on the good Karma!
917922
Well, if you want to measure their growth in milk you need:\n\nPure culture of the bacteria in question, sterile milk, sterile glass tubes and a spectrophotometer.  This will measure the density of the bacteria in liquid culture.  I don't know what equipment you have access too (pipetts, tubes, a spectrophotometer etc).\n\nIf you've isolated the bacteria from milk and want to just measure their growth in "whatever", you can use any sterile growth medium that supports the growth of the isolated bacteria.  Again, measuring growth is most easily accomplished by use of a spectrophotometer.  \n\nGrowing bacteria on a plate to decide which grows faster is fairly insane, unless they have significant differences in growth.  I mean, are you going to stare at an agar plate for 24 hours just to see that at 14 hours 5 minutes a tiny pinpoint on plate A was visible, but it took 15 hours and 22 minutes for a colony of the same size on plate B?  No, of course you're not, it's not even plausible.\n\nWith liquid culture, you can take readings every hour for 8 hours and at 24 hours or something.\n\nYou can also dilute and spread-plate the bacteria from liquid cultures onto agar plates if you have your heart set on using them and achieving an exact count for bacterial populations.  But use of a spec still allows for comparisons between species, even without an exact population number.
1114615
Well, apparently, they grow quite well on certain caterpillars ...\n\n:-P\n\n... but, seriously, AFAIK the bacterium is not too fussy, or (as we microbiologists generally call it) "fastidious" about its growth requirements.  I assume it will grow on any "minimal" medium, that is, maybe a bit of Yeast Exatract, + / or tryptone or meat extract, with or without addition of agar.  You did not say whether you wanted a liquid or solid growth medium.\n\nWe find Bacillus species as reasonably - common contaminants from the environment.  Our media are designed to isolate bacteria important to human health, so they are more nutritious than Bacillus thuringiensis would need (AFAIK).\n\nIf you do grow it, be careful with it; many members of this genus produce toxins.  I suspect that Bt toxin might not be very good for humans, too.  IMO, it is probably unwise to try to grow bacteria, unless you have appropriate training and laboratory equipment.  For example, you need to sterilise media and equipment, and properly dispose of wastes, in a safe fashion, and according to local bylaws etc.\n\nI am a microbiologist.
508821
no one cultures for viral respiratory infections , culturing is usually done for bacteria . viruses can be detected by doing blood test and checking for antibodies ( rarely done and expensive to do ), very little you can do to fight viral respiratory infections except rest , fluids and mild analgesics . antibiotics dont do anything for viral infections
196649
Several ways to do this. The first that occurs to me is to have the mammalian cell culture in a sealed chamber and measure the gases via IR spectroscopy in the chamber. The loss of oxygen in the gas of the chamber would be the uptake. Such methods are quite sensitive.
185951
The key problem in bacterial spores is lysis. You can use sonication (see 1st PubMed reference in Analytic Chemistry), followed by a standard DNA extraction protocol, which any molecular biology laboratory should be able to accomplish. (see 2nd website link)
668397
Depends if you want to grow pure cultures of microbes or not. If you don't have to have pure cultures, just put your leftovers in the fridge and forget'em. ;-)\n\nIf you want to grow pure cultures, you will need to know aseptic technique and what each type of microbe likes to eat. \n\nSome will go on a defined medium. That is one that you know exactly what it in there. Some have to have something complicated that we don't know exactly what all the components are, like yeast extract, beef heart infusion, that sort of thing.\n\nThen you need to know the temperature and humidity requirements of your bug and any special atmosphere conditions it need, like low oxygen or no oxygen.\n\nIt also helps to know how to store samples of your bug for future use. Can you freeze it? or do you have to lypholyse it?\n\nHope this helps.
1013746
So you'll need the right Bt strain - Bta or Btk; and not all commercial formulations of Bt contain living cells, so you’ll need some active colonies. I would guess that the nutrient broth that manufacturers use is proprietary. The trouble with entompathogens is that they lose virulence when grown outside of an insect. Manufacturers compensate by developing growth mediums that include insect parts or a blend of certain proteins, antibiotics, nutrients, etc. You can probably find some recipes in research journals but you’ll need to visit a university library or pay for a custom search. You can try Ward's Scientific for culturing supplies http://wardsci.com/  You may also need stirrers or shakers and a place suitable for sterile culture. If your broth gets contaminated with fungi or other bacteria you might just kill all of your bees.\n\nAfter you grow your bacteria, you’ll have to induce them into sporulating. The bacteria themselves do not affect insects; it’s the chemicals used in sporulation. After sporulation, you’re left with a bacterial spore, the husk of an empty bacterial cell and a crystal of toxin (it’s an insect stomach poison with no affect on humans). If done in the proper setting, you can harvest these crystalliferous remains, weigh them to calculate the proper dose and formulate it for easy application. It’s not a simple procedure.
1028100
bacteria,no question of doubt
1144967
Try the Internet and a Microbiology book
100001
First you have to determine the bacteria strain.
827835
Do it in a laminar flow hood. Follow aseptic techniques.\nDepending on your use, you may suspend your bacterial culture in an appropriate medium.\n\nIf you dont have a laminar flow hood, do it the traditional way...\nwash your hands, then put alcohol.\nwork on a clean table - clean it with alcohol.\nlight an alcohol lamp in the area to clean air.\nopen your culture tube near the light.\ntransfer aseptically the bacterial culture to cuvette\n  - do it near light to ensure no contaminants from air\ncover the cuvetter near the light.
57968
If you are trained in microbiology you can tell by eye.  Plate out the bacteria (for single colonies) onto a universal media that is suitable for your target bacteria,  ie CLED, Maconkeys, Blood, Chocolate.  Incubate until there is a growth (usually 24hrs at 37 degreees celcsius is aerobic).  Look at the plate, are the colonies uniform in appearence.\n\nIf you are using liquid culture youu could use microscopy before plating out.  Either a gram stain or a direct (wet prep). This would let you know if you had all gram positives or all gram negatives or all rods or all  cocci or a mixture.  You would still have to plate out to make sure only one colony type grows.
142615
Tryptic Soy Agar. It's the basic medium (food in a petri dish or test tube) for growing bacteria.
792062
1) Collect your microbial agent\n2) Inoculate it into enriched media\n3) Place 3 mm size ring of ointment onto the agar\n4) Check in 24 hours and measure the inhibition zone