Is this a eukaryotic or prokaryotic genome?
924396
It is very simple. Last band gives you that answer. You need to see G=G bond and it is follow by TAG. It is definatly eukaryotic.
874631
Eukaryotic DNA (from the giraffe) contains introns and exons.  The introns transcribed into the premature RNA, but are spliced out by enzymes in the giraffe resulting in mature RNA that codes for the enzyme.\n\nBacteria don't do this, their genes do not contain introns.  So if you clone a gene directly from a giraffe's DNA into ap lasmid and then into a bacteria you're going to get an RNA with all sorts of extra junk being translated into a non-functioning protein.\n\nTHe way we get around this is through the use of cDNA.  cDNA is generated from mature RNA by the enzyme reverse transcriptase.  Reverse transcriptase comes from retroviruses and turns RNA into dsDNA (called cDNA).  cDNA is the same as the original gene except that it lacks the introns, so it can be cloned into a bacteria and with luck express a functional protein.\n\nThere is also an issue of post-translational modifications that the bacteria may not do, or may do that it shouldn't.  But for the nature of the question, the answer is the original construct contained introns and the use of reverse transcriptase to generate cDNA from giraffe RNA would solve the problem.
17973
prokaryotic cells are not bounded by membranes while eukaryotic cells are bounded by membranes.
6888
First off jamesmusik84's is completely wrong. Please don't consider any of it. The second answer is along the right path, except I have no idea what the reference to antibiotic is suggesting and some viruses have RNA genomes but many more have DNA genomes. And as far as bacteria go, both RNA and DNA are present inside there cells.\n\nSo, the answer to your question. Let's start this way: Bacteria are small single-celled organisms. Unllike eukaryotic cells (like the ones that make up animals, plants, fungi and many single-celled organisms such as yeasts) which package their genetic material (DNA) in membrane structures called nuclei, bacteria (which are also referred to as prokaryotes) don't have a nucleus. Bacteria also don't have other organelles which are found in eukaryotes, such as endoplasmic reticulum, mitochandria, chloroplasts, golgi bodies, etc. But like eukaryotes, bacteria do have an outer lipid membrane.\n\nSo, you can think of bacteria of little fluid filled sacs. Along with eukaryotic, bacteria are an example of "cells" which are the smallest unit of living things on earth.\n\nViruses, on the other hand, do not have an outer membrane. They don't the capacity to make their own proteins. They don't have the capacity ot replicate or metabolize on their own -- of which bacteria (and eukaryotes) do. It's for this reason that we don't consider viruses "living" things. Viruses have to "infect" a cell -- injecting its genetic material inside -- and use the cell's metabolic machinery to reproduce.\n\nViruses are essentially small protein "capsules" that have a piece of genetic information packaged inside. Because viruses are required to infect a cell to propogate themselves, they are classified as obligate intra-cellular pathogens.\n\nViruses tend to only infect specific cell types. Some infect bacteria. Some infect plants. Others infect animals. The specificity of what a virus can infect is goes beyond whether they can infect bacteria, plants or animals. Usually viruses have to infect a very specific animal, plant or bacteria. And even in the case of viruses that infect animals and plants, not only do the infect specif animals, they are usually limited to a few cell types whithin that animal.\n\nTake HIV for example: to the best of my knowledge HIV only infects humans (i.e. the "H" in HIV -- Human Immunodeficiency Virus). But it doesn't just infect any human cell. HIV only infects white blood cells -- specifically a class of white blood cells called T cells (there are many other "classes" of white blood cells including such things as B cells, NK cells, neutrophils, basophils, eosinophils and macrophages).\n\nI hope this sheds some light on the matter.
327006
You're trying to say you have a synthetic chromosome. I would check all the experiments for contamination, and check the cells for endogenous expression before jumping to that conclusion. There are a lot of hurdles to leap, as mentioned in the other posts.
604610
Prokaryotes (from Old Greek pro- before + karyon nut or kernel, referring to the cell nucleus, + suffix -otos, pl. -otes; also spelled "procaryotes") are organisms without a cell nucleus (= karyon), or indeed any other membrane-bound organelles, in most cases unicellular (in rare cases, multicellular). This set of characteristics is distinct from eukaryotes (also spelled "eucaryotes"), organisms that have cell nuclei and may be variously unicellular or multicellular. The difference between the structure of prokaryotes and eukaryotes is so great that it is considered to be the most important distinction among groups of organisms. Most prokaryotes are bacteria, and the two terms are often treated as synonyms. However, Woese has proposed dividing prokaryotes into the Bacteria and Archaea (originally Eubacteria and Archaebacteria) because of the significant genetic differences between the two. This arrangement of Eukaryota, Bacteria, and Archaea is called the three-domain system. This replaces the two-empire system.\n\nhttp://en.wikipedia.org/wiki/Prokaryote\n\n\nA eukaryote (IPA: [juːˌkarɪəʊt]), also spelled eucaryote, is an organism with a complex cell or cells, in which the genetic material is organized into membrane-bound nucleus/nuclei. Eukaryotes comprise animals, plants, and fungi—which are mostly multicellular—as well as various other groups that are collectively classified as protists (many of which are unicellular). In contrast, prokaryotes are organisms, such as bacteria, that lack nuclei and other complex cell structures. Eukaryotes share a common origin, and are often treated formally as a superkingdom, empire, or domain. The name comes from the Greek ευ, meaning good, and κάρυον, meaning nut, in reference to the cell nucleus).\n\nhttp://en.wikipedia.org/wiki/Eukaryote\n\nMORE STUFF ON LINKS
37083
Actually, the genome is all genes along with non-coding (i.e junk and regulatory) DNA.  Genes alone make up one element of a genome.
1053947
no, because it is a defect on human chromosome 21, where 3 copies are made instead of 2. this has never been seen to occur in the genomes of other organisms.
211549
No, there isn't. Please please please bear in kind that helminths and fungi are EUKARYOTIC.
427095
prokaryotes have 70S ribosomes, each consisting of a (small) 30S and a (large) 50S subunit, whereas eukaryotes have 80S ribosomes, each consisting of a (small) 40S and a bound (large) 60S subunit. However, the ribosomes found in chloroplasts and mitochondria of eukaryotes are 70S, this being but one of the observations supporting the endosymbiotic theory.\nwhat do you mean by inclusive? Subspecies ?
359832
Prokaryotes\n1. Their DNA is not enclosed within a membrane.\n2. Their DNA is not associates with histones (the proteins that DNA wrap themselves around in eukaryotic cells)\n3. They lack organelles.\n4. Their cell walls almost always contain peptidoglycan.\n5. They divide through binary fission.\n\nAtomicQuasar, all bacteria are prokaryotes, and thanks for adding that the prokaryotes are smaller and simpler and have plasmid.\n\nEukaryotes\n1. Their DNA is found in the cell's nucleus.\n2. DNA can be found on multiple chromosomes.\n3. They have a number of membrane-enclosed organelles.  Including mitochrondia, endoplasmic reticulum, and Golgi complex.\n4. Their cell walls are simple in chemical make-up.\n5. They usually divide through mitosis.
1116614
since each amino acid has a specific t-RNA for it, eukaryotes must have about 64 of them or so. must be equal to the number of codon possible= 4^3=64 (4 for A,U,C,G and 3 for the number of them in each codon).
1182355
Remember this: \n\nRNA (A, U, G, C)\nDNA (T, A, C, G)\n\nDNA has sequence: T-C-A-T-C-G \nmRNA would be c) : A-G-U-A-G-C
131498
Eukaryotes contain membrane-bound nuclei and other organelles, while prokaryotes lack this membrane-bound nucleus. Prokaryotes are classified in two kingdoms separate from the eukaryotes. Eukaryotes are the type of cells that make up plants and one celled organisms, but not animals.\n\nBut there is a longer discription on:\nhttp://ks.essortment.com/prokaryoteseuka_rgbo.htm
801993
If this is a true genomic sequence, you can only start at a start codon, which is ATG.\n\nBut, this appears to be a template sequence, so we'll do transcription now:\n\nThe corresponding mRNA is:\nAUG AAU GGC UCU AAG AAG AAA UAG\n\nAUG is a valid start codon.  And by the way, that's methionine (Met or M).\n\nNow the rest:\nAAU=Asparagine (Asn or N)\nGGC=Glycine (Gly or G)\nUCU=Serine (Ser or S)\nAAG=Lysine (Lys or K)\nAAG=Lysine again (Lys or K)\nAAA=Lysine again (Lys or K)\nUAG=Stop codon\n\nSo the end sequence is Met-Asn-Gly-Ser-Lys-Lys-Lys, or in single-letters, MNGSKKK.
1091440
I really wish people who don't have a clue what they're talking about would stop taking guesses at the answer.\n\nAmoebae are EUKARYOTIC. \n\nThe cell contains a nucleus doesn't it? That is the definting characteristic of a eukaryote cell.\n\nDemeter actually posted a link to a website which states that amoeba is a eukaryote...... and then said that it was a prokaryote.\n\nDum dum dum dum dum.
751672
Eukaryotic cells (typical plant and animal cells) are considered to be more advanced than prokayotic cells. Their DNA is contained within a membrane bound nucleus and they contain membrane bound organelles such as mitochondria. They also contain a cytoskeleton which is a series of tubes and microfilaments which are involved with movement within the cell.\n\nProkaryotic cells (eg. bacteria) have no membrane bound nucleus - their DNA is restricted to a region called the nuceoid which is not membrane bound. They contain no membrane bound organelles and have no cytoskeleton. They do contain non membrane bound organelles such as ribosomes ( eukaryotes also contain these)\n\nHope this is of some help.