Why is it possible to use a DNA sequence to identify bacteria? plzzz dont copy from internet..?
340051
becouse each strain of bacteria have their own specialized DNA sequence
327076
The goal behind DNA barcoding is that anyone, regardless of training can obtain an organism in the field, sequence DNA, and identify it to species.  This would be done by building a database with almost every species ever described in it.\n\nMost traditional morphologists would not mind the creation of such a database in theory, but oppose it as impractical.  The cost will be quite daunting and will almost certainly draw from funds that could go to traditional studies (including genetic studies conducted in an evolutionary framework).  The ability of this section of gene (CO1) to universally distinguish species has been questioned.  The universality of primers has also been questioned.  The main fear is that it will be perceived as a replacement for traditional taxonomy.  Genetic distance is a very crude approximation of species' splits at best, and a meaningless distraction at worst.
584734
I gave a lecture on this recently and have had first hand experience with this technology in a lab, so let me give you a brief and easy summary to take with you about this topic about Recombinant DNA Technology:\n\nIn order to clone you need 2 things: Bacterial DNA (the circular form called a "plasmid"), and the DNA you want to clone (be it human, mouse, plant, other species etc)\n\nThen you will need to cut the Bacterial DNA with enzymes called "endonucleases" that work like scissors. (So imagine a circle and I take a pair of scissors and cut the circle at some point in it, b/c I want to insert the foreign DNA (non native DNA) where I had cut.) I will have to do the same with the foreign DNA and have endonucleases cut OUT the particular piece i want cloned. \n\nThen we can put the two pieces together, the cut circular plasmid DNA from the bacteria, and the CUT out piece of foreign DNA. \n\nWhen we put the two pieces together it is called "ligation", and it is done as a reaction in a tube with enzymes inside that "glue" the "sticky ends" of the bacteria DNA to the foreign DNA. \n\nAfter the reaction normally done in a 16 deg. Celsius water bath, we now will have "circular" plasmids with our Foreign DNA inserted into the bacterial DNA (the circle DNA)\n\nThen we would re-insert this DNA back into the bacterial host (that the circle DNA first came out of) in a process called "transformation", this is where the broth from the water bath we just discussed is grown in a growth medium at 37 deg. celsius so that the bacteria can grow and produce MORE of those circle plasmids with our foreign DNA inserted into it. \n\nThen we would "plate" these onto agar plates, and let them grow in incubators at 37 deg. Celsius over night until we get small colonies of bacteria on the plate. \n\nEach "mound" of bacteria (small like white dots on the plate) contains millions of copies of our gene (inserted in the circular plasmid DNA of the bacteria). \n\nNow we can take these small colonies, and harvest them further, by putting them in large Erlenmeyer flasks to be grown in the same "growth broth" over night on a shaker for 37 deg. celsius. \n\nAfter of which we can harvest, and purify that culture so that only the circular plasmids remain (this is a long and convuluted process so we won't go into it in detail here), and from there you can store those in a 4 deg. Celsius fridge and keep it in stock for further research purposes. \n\nHope that helps!
3494
Not yet, but you can use BLAST to look up information about a DNA or protein amino acid sequence.
303798
There are more described species of insects at present, but it's unlikely that this is representative of the actual diversity of the organisms.\n\nInsects are easier to distinguish using visible characters, and there's more economic impact of various insect species, so there's more incentive to work on them.  \n\nWhile there may be millions of different species of bacteria, they are harder to distinguish from each other, except possibly by comparing genetic material.  So no one has actually done the work necessary to categorize all of those species yet.
273434
There are multiple start codons, depending on the species.  But I'm pretty sure that there are always a few oddities in how different species might encode information is slightly non-standard ways.
913347
Bacteria have very few chromosomes compared to us, so there must be a very simple bacteria with only 1 chromosome.  Their chromosomes are circular and are called plasmids.
435695
One way that transformation can be helpful for bacteria is that this is a main way for the bacterium to gain genes for antibiotic resistance, environmental stress resistance (like heat or pH), or an advantage against the host's immune system.  Think of it as a big "fruit basket turnover" where one bacterium's DNA (along with its advantages) can be trasferred to another bacterium simply by the cells making contact.\n\nAnother example is that scientists use transformation as a tool in molecular biology.  We can put our DNA of interest into a bacterial cell (usually E. coli) and basically make it do whatever we want it to do.  It's a pretty handy trick, actually.
492660
as far as i know its used for DNA figerprinting in electophoresis (i didnt spell that right).  basically you use a gel like substance, put DNA fragments in it (mixed w. dye) and put this in a solution and hook the one end of the whole device to a negative battery side or something and the other end to a positive side and the DNA fragments will seperate and u can use it to match up DNA samples w. people etc.
842928
base on theory, yes. but practicaly, the problem is the sample of DNA, there is complete DNA code or not.
839821
Checked my bacteria program on my PDA and all that came up where....neisseria gonorrhoeae, neisseria meningitidis, moraxella catarrhalis and veillonella sp. \n\nFirst off, all I recall about those organisms is that they are aerobic cocci that are gram-negative. The exception being viellonella sp. as it is an anerobe. \n\nSecondly, I only looked for those traits I just mentioned, no idea if any of these meet your other criteria. \n\nThirdly, haven't you been doing tests all semester to narrow it down? The pathogens are limited to what you have studied, so it is not as the scope is that large. Try making a list of all of the organisms you have studied, turn it into a graph with all of the traits possible from all of the various tests...start filling in the blanks and you should have no problem figuring out which organism it is. Our specimen was contaminated and I found no problem determining both the assignment and the contaiminant using this method. \n\nPerhaps it is one of the organisms I mentioned above, it's very likely if you have studied any of them in Microbiology. But if not, use the method I described above and it really is just a process of elimination. \n\nGood luck
861457
Those answsers are wrong. It is a bacteria that lives on an aphid. Here is the link to prove it:\n\nhttp://phylogenomics.blogspot.com/2006/10/worlds-smallest-genome-of-cellular.html
474656
If you are talking about bacteria such as E Coli, you need to demonstrate that the markers are close to each other. To do that you need to do mating involving the F factor and demonstrate that the linked genes transfere near to each other.  This was done in classical expts in the 40s and 50s.
125186
(I just did this today in lab with bacteria instead of onions!) Other liquids can do many things to DNA, but water binds to the DNA and "elutes" it, the DNA will stay with the water. (look up "elute")
412034
There are various methods to sequence.\nThe current method is have a primer that can anneal to a segment prior to the region you wish to sequence. You then extend the primer using a polymerase, dNTPs and fluorescent terminating nucleotides.  Once the fluorescent terminating nucleotide is incorporated the sequencing is terminated for that sequence.  You will end up with a collection of DNA fragments of different lengths that are a copy of the DNA you wish to sequnce.  These fragments are run through a gel and separated based on size.  Each terminating fluorescent nucleotide is a different color for A, C, G and T.  Based on which fluorsecent nucleotide is present at position you know the nucleotide that is there due to the color. \nFrom this you get the DNA sequence.\nWasn't that easy!
937369
No, not directly.  Bacteria are of the order of single celled organisms called prokaryotes, we are multicellular organisms of the order eukaryote.  We share basic similarities such as DNA, RNA, certain cell makeup but our genetic orders split into two different directions at a very early stage.  The genetic relationship stopped there.\n\nWe do share a symbiotic relationship with bacteria in our bodies and the world around us if that's what you mean.  We rely on bacteria to help break down food and control digestion.  Without that relationship we'd die.
116715
bacteria are prokaryotic(no nucleus)