what is immunofixation electrophoresis?
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Electrophoresis is a process in which an electric current is applied to a substance to separate it into its component parts.  In immunofixation electrophoresis, iy is the immunoglobulins that are being separated.  I am a little bit rusty, it has been several years since I have done this testing but there are 4 or 5 classes of immunoglobilins, IgA, IgE, IgG, IgM and maybe one more, I'm not sure.  This test is the definitive diagnostic procedur for multiple myeloma.  In multiple myeloma one class of lymphocyte, the white cells that produce antibodies, goes crazy and overproduces one certain immunoglobulin to an extreme.  Immunofixation electropherisis can determine if one certain immunoglobulin is being over produced.  One other test is diagnostic for multiple myeloma, this is the Bence-Jones Protein test performed on a urine sample.  Bence-Jones protein is the actual immunoglobulin that is produced by mutiple myeloma.
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usually protein with some metallic ions
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http://www.accessexcellence.org/AE/AEPC/WWC/1993/serial.html\n\n\nhttp://www.bio.umass.edu/micro/immunology/elisa/serial.htm
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It is not chemistry.  There is some unknown trigger that causes your body (B cells that have differentiated into plasma cells to be specific)to begin producing antibodies to your own proteins or DNA or RNA, etc.  These antibodies are called autoantibodies.
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NINHYDRIN TEST\n(glycine)Purple liquid\n(gelatin)Clear, colorless liquid\n(albumin)Bluish liquid with purple cloudy layers\n(tyrosine)Violet liquid\n(casein)Powder-like precipitate at the bottom light purple liquid.\n\nNinhydrin test is a product of the unusual reaction is a purple colored imino derivative, which provides as useful color test for the proteins or amino acids. The purple color indicates the presence of proteins on amino acids. Since the entire reagent gave the result of a violet color so all the reagents are proteins.
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The most popular sandwich assays are enzyme-linked immunosorbent assays (ELISAs), although newer assays, such as those using flow cytometry or based on amplification of the sample or its signal, are challenging their dominance.
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fluorescent microscopy will work for this.  First fix the cells (3% paraformaldehyde) then permeabilize (0.5% triton-x) and then stain with antibody to your protein of interest.
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I think it is a mechanical technique.  The powder simply sticks in the oils from the skin left behind on the surface. I am not up on modern techniques, but perhaps some chemical reaction between the powder and some treated paper allows investigators to make copies of the prints.\n\nElectrophoresis is a technique where an applied electric field causes charged molecules to move through a medium; usually a gel these days but paper used to be used.  Larger molecules tend to move more slowly, and more highly charged molecules will move more quickly, so you can separate compounds.  I don't see how this might be used in fingerprinting.
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DNA comparison by electrophoresis. I've seen kits available in school divisions for science classes. On a similar note: paper chromatography to check if the composition of ink between samples are same or different.
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okay I'm gonna try this. :-)\n\nImmunohistochemistry is a way to detect certain proteins that are in your body's tissues.\n\nIn kidney diseases, there may be abnormal proteins in the kidney that you'll want to look for.\n\nSo what happens is a scientific technician will take an extremely thin slice of kidney tissue, and treat it with "antibodies" to the abnormal (bad) protein.  \n\n[An antibody is something that will stick to a certain protein, they can be made to be very selective.  So there is a specially designed antibody that will find this bad protein and stick to it.]\n\nIn addition, this antibody has a "signal" stuck to it.  Normally it's some kind of fluorescent (shiny, hehe) dye.  After treating the sample with antibodies and getting them stuck to the abnormal proteins in the tissue, the technician will then perform a reaction that will activate the dye.  Then, the sample is put under a microscope, and the picture is analyzed for presence of this dye.  If the kidney is diseased and has the abnormal "bad" protein, there will be colored dye spots all over the picture.\n\nSo in short... kidney diseases can show abnormal (bad) proteins, scientists can make things ("antibodies") that stick to these bad proteins, and then these antibodies can be "activated" to light up the dye and reveal where the bad proteins (which they are sticking to!) are.\n\nIn theory, a normal kidney tissue sample would not show any fluorescence/lighting if they were looking for a "bad" protein.  Of course, "bad" protein is a huge generalization, and I'm just trying to simplify it into layman's terms. \n\nContact me if you have other questions, hope this helps!\n\n-IJ
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first you will need the blood sample, anti-A antibody and anti-B antibody. \nplace a drop of anti-A and anti-B antibody on separate slides.\nsmall amount of blood is taken from the patient's 4th finger. the blood sample is then mixed with the droplets of the antibodies.\nafter a few minutes, observe any signs of agglutination on the slides. if agglutination occurs on slide with anti-A antibody, the blood contains antigen A (blood type A). if agglutination occurs on slide with anti-B antibody, the blood contains antigen B (blood type B). if there's agglutination on both slides, the person has blood type AB. if there's no agglutination, the person has blood type O.
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Typically labs have densitometers for gel electrophoresis analysis.  There has also been increasing use of capillary electrophoresis systems for IFE/s and SPE.
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gel electrophoresis can be used in forensic science as well as in maternity@paternity testing.here wat they do is from the place where the crime has occured if they get some evidence like a bit of hair, or spitted saliva or a small amount of blood any thing else they usually collect these sample they usually extract dna,with tat they set@ioad    withsamples of that particular victim and they usualy have collection of samples from the crime they will run the sample of the victimie the criminal @those of with the suspect.usually in gels we get bands at particular position,if there are identical band found in any one of the suspect @the victim then he is the criminal.
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It is called biochemestry
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Hemoglobin electrophoresis is the best method, but it requires fetal blood. If you and your baby's father are both B-Thal minor, then there is a problem. Otherwise, the Dr will probably choose to do the test at delivery.
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That depends whether you're doing a Southern, Northern, or Western blot, and with what.\n\nThese are ways of exposing a probe to a target to see if two things interact with each other. A Southern looks for DNA interactions, a Northern uses RNA, and a Western uses protein. The blot contains the immobilized target attached to the nitrocellulose membrane and the probe attached to the target, plus whatever is used to visualize the interaction, which could be something that generates a color reaction, a fluorescent label, or a radioactive label.
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I believe you're asking about a monoclonal antibody. Perhaps when this was copied the "ocl" looked like a "d" and the "n" looked like an "r."\n\nhttp://www.bartleby.com/65/mo/monoclona.html