why are control buffer cells plated on nutrient agar?
355307
It isn't a very good control if your cells die before the experiment is complete.  More info is needed to get a more specific answer.
86313
Each cell is like a plastic bag filled with a spaghetti soup of all the different cell components. But it's a very special plastic bag, because it is permeable - water and friendly chemicals can pass through it, but bad guys get shut out. Rather like the firewall works (or should work!) when you're on the internet. The cell membrane is the plastic of the bag.\n\n:-)\nDC\nwww.tigerworld.tv
141967
The well in the chamber is to contain the buffer solution. The well in the agarose is to contain the sample.\n is that what you needed??
759644
Who said that??!?!? Medcines are sterlized with measured quality control standerds to prevent the presence of ANY harmful micro-organisms,(I.V ampules are more "strictly" sterlized than capsules and pills)
33397
Nature has determined this design to be the most efficient and useful for higher order organisms.  There are two types of cell "designs", prokaryotic (bacteria) and eukaryotic (plant and animal cells).  It is thought that at one point all living things were prokaryotic, then at some point in our evolution a nuclear envelope was developed and proved to be beneficial to the organism, thereby natural selection allowed for the continuation of a nuclear envelope.  As time went by plants and animals both evolved from some ancestor of this first eukaryotic cell and henceforth have similar cell designs.  As for the actual benefits of this design, the nuclear envelope of plant and animal cells serve to protect the DNA, and therefore genes, of the animal from damage, and also serves as a type of filter seperating cellular processess in the cytosol from gene regulation in the nucleus.
510413
Egg white are analogous.  It is a mechanical fluid protecting the yolk/cell nucleus.  \n\nIn the cell, the cytoplasm is a sort of warehouse where in-bound nutrients and out-bound wastes are processed and positioned for transit.  \n\nCytoplasm is sort of a bank of protective and equalizing fluids, a kind of checking account, taking deposits from the outside of food and fluids and spending them on the directions of the DNA/RNA and various cell organelles as they call for them.
883939
polysaccharides?\nhttp://www.cellsalive.com/cells/cellwall.htm\n\ncellulose?\nhttp://www.usoe.k12.ut.us/curr/science/sciber00/7th/cells/sciber/cellwall.htm
415279
Nutrient broth ingredients:\n\nMeat extract 10.0 gm\nPeptone 10.0 gm\nSodium chloride 5.0 gm\nDistilled water 1000 ml\nMix the ingredients and dissolve them by heating in a steamer. When cool, adjust the pH to 7.5-7.6.\n\nTo make it agar add:\nTo the ingredients as in nutrient broth, add 15 gm agar per litre. Dissolve the agar in nutrient broth and sterilize by autoclaving at 121oC for 15 minutes. Prepare plates and slopes as required.
773115
you can surely rule out some things based on the type of plate you are using.  assuming that you have covered that base, you can separately pick a colony that you think may be a contaminant and look for differences in growth patterns, color, morphology, gram stain, growth on other media, and if all else fails, you can sequence it to find out exactly what it is.
440639
to stop condensation forming on the lid and dropping into the agar
628459
You're trying to design a medium?  Your question is mighty unclear.  \n\nAnyway, you include toxic compounds for many reasons, the main one is that if you have a particular bacterium engineered to to do a particular function, you design a co-effecting gene that permits the bacterium live in the presence of the toxic compund, and die if it loses the ability to survive in it.  This ensures that if the engineered genes are unstable, both genes are likely to fail, thus any defects or errors in the plasmid or inserted sequence that does your valuable work and allows it to survive in the toxic compounds will kill the bad batches.\n\npH indicators you insert for certain types of bacteria to determine the age (if acid or basic products are developed from the metabolism), resource availability (i.e. if the culture needs to be transferred to new media) and if what you have is contaminated or not (i.e. if you have a sample that's supposed to be always neutral or at a certain pH and suddenly your indicators start going bananas... not good.)\n\nAnd for the rest, you design differential/selective media for the single purpose of making sure your samples are pure and devoid of other species (eliminates the competition) and that if anything goes wrong, that it doesn't go wrong with your whole batch.  \n\nAlways flame your loops :)
609978
When water enters the cell..it must be regulated or the cell will...em...explode...these vacuoles help the paramecium to send out extra water...
275373
MH agar is more stabilized/buffered media so that byproducts of bacteria can not change pH and hence efficacy of antibiotics tested. \nMH is not the only media to test susceptibility, some organisms need more nutrients and Blood Agar is used to test susceptibility.
1027129
Agar plates are used to grow and study bacteria by spreading a thin solution of bacteria onto the agar (a gel with the consistency of jello).  LB stands for Luria Broth, food for the bacteria to grow.  Amp stands for Ampicillin, an anti-biotic (kills bacteria).  And 100 is the concentration of Ampicillin in the gel, 100 ug/mL.\n\nSo, what kind of experiment could you do with LB Amp 100?  Well, let's say you had some bacteria that would normally be killed by Ampicillin.  If you put them on the plate and incubated (heated) them, you would see nothing because all the bacteria would be killed.  Now, if you take another set of bacteria that has a gene that makes them resistant to Ampicillin, and plate and incubate them... you would see little spots (colonies) of bacteria growing.\n\nOf course, there are many more experiments you can do.  But that gives you an idea.  Hope that helps.
815455
blocked pores and bacteria.
57968
If you are trained in microbiology you can tell by eye.  Plate out the bacteria (for single colonies) onto a universal media that is suitable for your target bacteria,  ie CLED, Maconkeys, Blood, Chocolate.  Incubate until there is a growth (usually 24hrs at 37 degreees celcsius is aerobic).  Look at the plate, are the colonies uniform in appearence.\n\nIf you are using liquid culture youu could use microscopy before plating out.  Either a gram stain or a direct (wet prep). This would let you know if you had all gram positives or all gram negatives or all rods or all  cocci or a mixture.  You would still have to plate out to make sure only one colony type grows.
1082486
Glomerular capillary filtration is sensitive to ionic charge of a compound being passed through it. this is why complex proteins become trapped in the basement membranes and appear as electron dense deposits in tissue sections examined by electronmicroscopy. ionic charge of lipid is repelled