could you please explain southern blotting?
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Detection of specific DNA fragments by gel-transfer hybridization (Southern blotting). (A) The mixture of double-stranded DNA fragments generated by restriction nuclease treatment of DNA is separated according to length by electrophoresis. (B) A sheet of either nitrocellulose paper or nylon paper is laid over the gel, and the separated DNA fragments are transferred to the sheet by blotting. The gel is supported on a layer of sponge in a bath of alkali solution, and the buffer is sucked through the gel and the nitrocellulose paper by paper towels stacked on top of the nitrocellulose. As the buffer is sucked through, it denatures the DNA and transfers the single-stranded fragments from the gel to the surface of the nitrocellulose sheet, where they adhere firmly. This transfer is necessary to keep the DNA firmly in place while the hybridization procedure (D) is carrried out. (C) The nitrocellulose sheet is carefully peeled off the gel. (D) The sheet containing the bound single-stranded DNA fragments is placed in a sealed plastic bag together with buffer containing a radioactively labeled DNA probe specific for the required DNA sequence. The sheet is exposed for a prolonged period to the probe under conditions favoring hybridization. (E) The sheet is removed from the bag and washed thoroughly, so that only probe molecules that have hybridized to the DNA on the paper remain attached. After autoradiography, the DNA that has hybridized to the labeled probe will show up as bands on the autoradiograph. An adaptation of this technique to detect specific sequences in RNA is called Northern blotting. In this case mRNA molecules are electrophoresed through the gel and the probe is usually a single-stranded DNA molecule.
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these might help..\n\nhttp://www.blackwell-synergy.com/doi/abs/10.1111/j.1365-2621.2006.01347.x\n\nhttp://www.co-lipidology.com/pt/re/colipidology/abstract.00041433-200502000-00009.htm;jsessionid=Fh8fr0yV02g1pkF1LGYLXyzy9lPXZ4Gn69QZKHJ1GG1vJYGw1czy!-1640309041!-949856145!8091!-1\n\nhttp://scholar.google.com/scholar?hl=en&lr=&q=cache:yYCPrA4fU54J:bmc.ub.uni-potsdam.de/cgi-bin/show.pl%3F1475-2891-4-33/1475-2891-4-33.pdf+list+of++biochem+experiments,+TEAC+assay+or+the+phenolics+test
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southern united states
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Well let me be the first to say hahahahahahaha......because I'm from south MS and we southerners "use it all the time". ex: I'ma do it afterwhile, I'ma tell it if u don't, I'ma see if she's home....etc. rather than say I'm going to or about to; we say I'ma or Fin too, or finin, finna etc...but surprisingly I never say fixing too....if anymore southern slang boggles your mind.........just ask.........take care
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We run Western blots all the time in our laboratory-you will need to specify exactly what you are asking here
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take an epindorph tube, place about 500 microlitres of blood, centrifuge, remove supernatent, wash with PBS buffer, repeat 3x, by an RNA isolation kit from roche and foolow the instruction!!
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HEY U R IN WHICH STD??AND WHTS UR SYLLABASE..WHT IS PSLE??\nI HAVE GOT SOME SAMPLES BUT NOT SURE WTHERE IT SUITS UR INFO??CAN U PLZZ EXPLAIN WHT I ASKED.\nis it pole scene or psl science??
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http://southernfood.about.com/library/info/blfluidmeasure.htm
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Are you looking at any protein in particular?  Look up in situ hybrdization for proteins ( at least it will give you an idea where it is localised).  I think that it would really depend on the system ad proteins that you want to study, so I am not sure how much else I can tell you, sorry.
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You gave very little info so this is only a guess.\n\nMaybe this patient had some kind of hypersensitivity reaction.\n\nNot sure.
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may be bleaching is the answer
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http://chemistry.about.com/library/weekly/blsapon.htm\n\n\n\nSimple web search
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One is a simple binding assay, in which the ligand (usually radiolabeled) is added to a hemogenate of the tissue-a number of washes are done, in the presence of cold ligand, in order to retain only that ligand that has bound with high affinity.\nThe others are all protein-based.  Western blotting with specific antibodies, immunohistochemistry to determine the presence and cellular localization.  RT-PCR or Northern blotting will tell you if the mRNA is present, but not if the receptor protein itself it there.
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The over simplified answer: all the yolk is concentrated at the bottom pole (I forget if this is called 'south' or 'negative' or whatever). This isn't the only difference, but its the first obvious one during development.
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I would have to guess that it involves the neutralization reaction of more than 2 substances.\n\nJust a guess
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I am not sure what you are asking.  AP?  Alkaline phosphatase?  SDS-PAGE - Sodium Dodecyl Sulfate Polyacrylamide Gel Electrophoresis?\nSDS-PAGE separes proteins according to their sizes.  Large proteins migrate slower than small proteins.  Once the proteins are separated, a size marker next to the protein can identify its size.  Western-blot can be used to detect the protein with antibodies that recognizes the protein.  There are also antibodies that recognize phosphorylated form of the protein.
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can you elucidate it a little more please?