what mediums,preferably easily available,can b used to grow bacteria? household materials are also preferable.mediums that will grow the culture relatively fast.
206451
There  ate tons of mediums commercially available to culture bacteria, they come in the from of liquid (broth) or  solid media (agar). They can be non selective, selective or to differentiate.\nBut is you are looking for a household material you can use  baby food or gelatin. It depends on what you want to cultivate.\n For example if you use the baby food there will also grow fungi and other microorganism.
216289
Actually, your teacher is wrong.  It all depends on the growth phase of the bacterial culture.   There are 3 phases in the growth of a bacterial culture; the lag phase, the log phase, and the stationary phase.  The lag phase is exactly what it describes...the bacteria divide veeeeerrrryyy slowly.  In simple terms, this is mainly due to low amounts of certain "growth signals".  It usually takes bacteria several hours to get out of lag phase and into log phase.  \n\nLog phase is the stage where the bacteria are dividing at a constant rate.  This is when you're going to take measurements for the growth rate.  Say, for example, that your cells have a doubling time of 1hr.  So if your beginning OD is 0.8 your end OD after an hour would be 1.6.  In this case B is twice b.  However using the same example, if we grow for 2 hours instead of 1, you've gone thorugh 2 doubling times and the final OD would be 3.2 verses 0.8 (B is 4x b).  So the relation between b and B is strictly dependant on the time (which is why "t" is a component of the equation) and growth rate of the cells.\n\nThe final phase of bacterial growth is the stationary phase.  This is where the number of bacteria increases to such a high density that further division is inhibited (by physical limitations such as nutrients, pH, cell/cell inhibition, etc) and the growth substatially slows.\n\nI hope this clearly answers your question.
617634
there are many biological tools. few of them are,\n\nIncubators : which are used for growing microbes on laboratory media...they are maintaiined at different temperatures for bacteria, fungi and algae.\n\nLaminar air flow and saftey cabinets: used for carrying out microbiological work aseptically.\n\nAutoclave : Used for sterilization of microbiological medias\n\nBalances : Used for weighing media's\n\nhope that's enough for you.
167019
There are both culture-dependent and culture-independent ways of doing this...\n\nCulture-dependent: you can centrifuge a soil sample and then plate out the supernantant onto various types of agar.  If you are looking for bacteria, you can use agars that enrich for certain species like Streptomyces or Actinomyces Isolation Agar, or you can use more general agar like Tryptic Soy Agar.  Just be sure to include cyclohexamide in the media to prevent fungi from taking over!  If you are looking for viruses, you will have to do a virus/phage prep from the soil supernant and then plate onto lawns of bacteria or fungi, depending on what types of viruses you are looking for.  If you are looking for fungi, you can use the same supernatant but use media for fungi enrichment, such as Potato Dextrose Agar or Worts Agar.\n\nCulture-independent:  you can isolate DNA from the soil and do sequence analysis to see what kind of bacterial/viral/fungal commuunity there is in the soil.  There are various kits you can buy to do this, dependding on which organism's DNA you want.  This is a much faster method than growing them on agar, but you miss out actually seeing the organisms, as well as enumerating them.
519517
your taking microbio hu? well good luck. H2O2 doesn't damage cells but it does kill bacteria that can't stand too much oxygen.
609125
This is a great source for you:\nhttp://www.blackwellpublishing.com/ogunseitan/student.asp\nEnjoy the microbes!\n;-D
417823
1- Some bacteria can photosynthesize, like cyanobacter,  the bacteria that makes vitamin B12, cyanocobalamine. \n\n2- Some bacteria have an outer membrane that gives them their shape just like cell wall in plants.
646756
check out hydroponics, soilless growth, with sodium halide lamps. Amazing growth rate but expensive for electricity. you can find the right kit on ebay. happy growing, man:)
503053
Firstly, is the culture tube to be a nutrient broth or a nutrient agar?\n\nWhichever it is, both will have to be autoclaved to remove all other organisms. After autoclaving, don't open until you want to insert the sample. Make sure you have a naked flame in front of you. The area just around the flame is your sterile area. \n\nFor a nutrient agar, heat your innoculating loop in the flame until it is red-hot then remove from the flame and flick it to cool it. Test on one side of the agar to make sure it is not too hot. If too hot, the agar will hiss. Collect your sample of E. coli with the innoculating loop and use your other hand to hold the culture tube. Open the culture tube with as few fingers as possible. Streak the E. coli on the nutrient agar making sure to spread it evenly. Always keep the mouth of the tube near the flame. The innoculating loop should also always be near the flame until the process is complete. Close the culture tube and heat the innoculating loop again.\n\nIf it is nutrient broth, then follow the same steps as above except that you insert the innoculating loop into the broth and shake it. Then close as above.\n\n*Remember... when opening the tubes, always keep the mouth of the tube close to the flame and always sterilise the innoculating loops.*\n\nEDIT: Answer to the replied question.\n\nYeah a micropipette would be easier. You'll need to autoclave the micropipette tips before using and like I said, all the procedures have to be carried out right beside the flame. The mouth of the culture tube has to be in or right beside the flame so that no contamination happens.\n\nIn any case, the innoculating loop or the pipette can be used because the nutrient broth is there for the bacteria to develop colonies and grow so the end result will still be the same.
629816
It is only A, Bacteria that applies to your question.
343961
macroscopic. you "farm out" the bacteria on a agar plate, then describe what grows (usually after 48 hours, but can both be more or less- depending on the species)\nNote: there are VERY many possible culture media (usually Agar based), and some bacteria will grow only on a few of them- that helps with the ID\nAlso- some bacteria need special culture conditions- eg. anaerobes- which die if they are cultured in contact with air. Also helps with the ID.\n\nMicroscopic. First of all look at the bacteria under a microscope- that gives you some info.- eg are they round (cocci) or elongated (bacili) or curved (vibrio) or spiral, or any of a multitude of shapes.\nsome bacteria are transparent and can only be seen in a "dark field" (reflected light from bacteria seen on an otherwise unlighted space) typical for Treponema pallidum- causes syphilis.\n\nThen the bacteria can be stained- very many possible stains and methods- but the most commonly known is the Gram stain (hence some bacteria are called "Gram positive" or Gram negative" - depending if the stain "caught" on the cell wall.\nSome stains are then removed with acid preparations- and in some bacteria it works, in others not. eg. the TB bacteria are known as "acid fast"- meaning an acid bath does not remove the colour of the original stain\n\nEssentially you are supposed to go through all the steps and look up in a book what fits your particular bug.\nThe bug can also be a fungus- yeast or mould
195113
One way that transformation can be helpful for bacteria is that this is a main way for the bacterium to gain genes for antibiotic resistance, environmental stress resistance (like heat or pH), or an advantage against the host's immune system.  Think of it as a big "fruit basket turnover" where one bacterium's DNA (along with its advantages) can be trasferred to another bacterium simply by the cells making contact.\n\nAnother example is that scientists use transformation as a tool in molecular biology.  We can put our DNA of interest into a bacterial cell (usually E. coli) and basically make it do whatever we want it to do.  It's a pretty handy trick, actually.
405556
Bacteria and viruses vary greatly in size- bacteria can be anywhere from 10 to 100 times larger than viruses. Basically, bacteria contain everything for them to reproduce- genetic information in the form of DNA or RNA, ribosomes (necessary for translating the genetic information into enzymes required for its survival), and plasmids which convey extra genetic information for a variety of purposes (bacterial "sex" for example- bacteria normally reproduce asexually, but with certain plasmids genetic material can be passed between bacteria). Viruses, on the other hand, generally only have genetic information, have no way of replicating that information and also lack the machinery needed for making its own proteins. They need to infiltrate living cells to be able to reproduce. You may have seen pictures of viruses looking like moon-landers- the legs attach onto the surface of the cell, then the genetic material contained inside the head of the virus is injected into the cell. This genetic material can either use the cell's machinery to produce its own proteins and/or virus bits, or it can be integrated into the cell's DNA/RNA and then translated later. When enough "baby" viruses are produced the cell bursts, releasing the new viral particles.\n\nThey also differ in structure- bacteria have cell walls (in addition to their cell membranes), which contain peptidoglycan polymers and provide structural definition. Another answer mentioned the fact that antibiotics can kill bacteria but not viruses. This is because many antibiotics interfere with the production of peptidoglycans for cell walls. Viruses contain a simple protein coat on the outside. They lack peptidoglycans, therefore are not affected by antibiotics.
401443
A living cell (like a cell from the liver, lungs, brain, etc...) will die soon after it was removed from its original "place" (liver, lungs, brain, etc.) as it has no more nutrients-supply (by blood venes, for example!) except it is kept in an artificial environment, a culture medium, which is suited to it's very specific needs.\n\nA living cell that is an organism needs a special suited environment, too (a diatom won't survive in the desert...). But it is not depending on an EXTERNAL supply of nutrients, as it "searches" its nutrients on its own. Bacteria, for example, don't need blood venes to grow in order to make cell division, they just need an environment which supplies all the needed nutrients. That's why bacteria can grow nearly everywhere, while lung cells  would die short after you dispersed them on an old dank sponge.\n\nHope I helped you. Excuse my English, please, it's not my first language. Good luck for tomorrow! ;-)
114895
what are you looking for exactly. how to grow it at how . give me some details and iam sure i can help.
329836
http://www.sigmaaldrich.com/Area_of_Interest/Analytical__Chromatography/Microbiology/Product_Lines/Culture_Media_Index.html has a listing of different culture media.
148887
PBS usually comes sterile and it has a salt balance that can prevent any hyoer/hypotonic situations, but it is not always used to make growth media. Actually, I have used PBS in molecular techniques, but never in making media.