i'm looking for a protease assay for a science lab. Our teacher is going to give us unknown samples and we have to find out which one contains protease. We have to find a lab that will help us find protease in a sample. we're not allowed to use kits. I tried searching on google, but all I find are kits. Please help ASAP!
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is something about hiv... from what i know. it is a game but it might work. u shout to the teacher to make him pay attention to you and when he looks at you, u take a sample in your hand and try to drink it. the teacher will then say that is something in that sample or not.
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Whenever I pellet DNA in the lab it often comes down in a white pellet because of the salts we use to precipitate it. This may or may not be contamination depending on your point of view and what you are trying to do (for us it is not contamination).
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I use a 1 KB DNA ladder for electrophoresis and it comes with a paper to actually tell you which band represents which size fragment. Maybe you could look on the tech company's website. If you do not need to know the actual fragment size of each band, maybe you could just compare to make sure the band you need is there in the first place.
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:) Thanks for taking all the trouble in finding me to send me a question. I Feel flattered. I am not a chemist..but i would suggest that you try searching google/yahoo with the following word combination:\n1. "Voltaic cell"+experiment+laboratory\n2. "Voltaic cel"+"kitchen laboratory"\n\nand so on.. I am sure you will find some answers. good luck! I will try to pull out more answers and try to send them to you. by the way my email id is same as the handle..scientist_atlarge.
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Hi purplepansy.  Cute 'name'.  Amino acid imbalances are common and can cause significant health problems.  Getting tested, therefore, may be a very good idea.\n\nUnfortunately, because I am not aware of the saliva test your doctor is recommending, I can't give you any advice on that.  Ask your doctor this question.  I am sure he/she will be happy to answer your concerns and provide you with documentation about the test's accuracy, reliability, etc.\n\nIf you are really in doubt, have BOTH tests done.  Serum amino acid profiles are valid and would provide a good comparison for the saliva test.  If the results are similar, then you can do future/followup testing with the saliva method.\n\nBest wishes and good luck.\n\nP.S.  I just received the results for my own serum amino acid profile yesterday.  I get mine tested (along with many other tests) annually.  I use Metamatrix Labs and their ION profile.
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Since we don't know what "this lab" or "this investigation" refers to, we probably can't really help you.  Try asking a classmate or the teacher for help, since they know what "this" refers to.
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Here are some Lab Apparatus \nhttp://library.advanced.org/11430/research/apparatus.htm\nhttp://www.charltonsci.co.uk/charlton_scientific_015.htm\n\nhere are all the Lab Apparatus (click on them to get info one them)\nhttp://en.wikipedia.org/wiki/Category:Laboratory_equipment
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I can definitely tell you the enzyme is not an initiator. The DNA polymerase has a sequence that initiates replication. \n\nThe protein you are talking about is called SSB (Single Starnd Binding) protein. I am very amazed where you got this question from. I have never before heard such a function related name for SSB protein. But if it is a must, I would go for straightener since SSB unbound single starnded DNA forms folds (loops) as in RNA. But it definitely is not initiator.
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Walk over to the science lab and ask one of the professors, or if you are a middle school student, ask one of the teachers.  I am assuming this is for a legal purpose of course.
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try this\n\nhttp://en.wikipedia.org/wiki/Category:Laboratory_equipment
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http://www.fhcrc.org/science/shared_resources/proteomics/peptide_synthesis/basics/spps.html\nTry this one. I hope it helps
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Maybe.\n\nSerum protein electrophoresis generally separates the proteins into five distinct bands.  For such different creatures, I would expect the population of the various bands to be fairly different, and therefore it would be a good tool to distinguish between those different species, I suppose.\n\nIf, on the other hand, you wanted to compare SPECIFIC proteins in these different creatures, electrophoresis would probably be a poor way to get a good sample.  I'm thinking the right kind of chromatograph or a targetted binding reaction would be better for that.
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Resources for the student including tutorials, animations, project ideas, and sample quizzes.\n\nPhysics Classroom Projects\n\nThese projects were designed for my College Prep, Honors and  AP Physics B students\n\nAll Science Fair Projects Over 500 FREE Science Fair Project Ideas!
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You might want to use different paper...   i would get the blotting paper from a blot spot, they'll have variants.
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There are so many different enzymes in your body. They are mostly for digesting food. Like in saliva, you have amalayse which starts the breakdown of food. You can get more info at webmd.com.
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Do you need to convince a lab to investigate tardigrades? Or are you looking to join a lab?\n\nThere are very few tardigrade biologists who also do molecular work. I have found this guy for you:\n\nDr Garey http://chuma.cas.usf.edu/~garey\n\nHe seems to be a big fan of tardigrades, and if you can't join his lab (which would only be due to some sort of visa problem) he should be able to give you the names of some other profs. He ought to know, since he ran the Ninth International Tardigrade Symposium in 2003.
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A large number of biological compounds can be separated by thin layer chromatography (TLC). The specificity comes from the type of substrate that is deposited on the plates to use for separation and then the solvent systems that are used to develop the plates and separate the compounds.\n\nOne of the commonly used methods in research labs today is to differentiate between lipids or amino acids that have been phosphorylated by the action of a kinase enzyme. For instance, the presence of a newly added phosphate molecule onto a lipid like phosphatidyl inositol will create a new compound that can be separated from the original by TLC. Another example is when a protein is phosphorylated by the action of a kinase, you can digest the protein and compare it to an untreated sample and you will find a new spot corresponding to the phosphorylated peptide.\n\nDifferent types of sugars can also be separated by TLC. As can metabolites of various biological reactions. It is a little difficult to separate full-length intact proteins by TLC, but it is not impossible. The key features boil down to the choice of substrate and the mobile phase. In forensics, while I am not certain (not my area of expertise), I am relatively confident that drug metabolites can be isolated and verified by TLC.\n\nWhile TLC has a number of uses, it is most traditionally used to characterize substances that have undergone some sort of chemical alteration so that they can be identified when compared to an untreated sample of the same substance, for example, one sample plus or minus the addition of phosphate.