how to do afb staining?
153319
['Step 2: Smear Preparation (Review) Add one loopful of sterile water to a microscope slide. ... ', 'Cover the smear with carbolfuchsin dye. ... ', 'Dry heat for 2 minutes.', 'Cool and rinse with water. ... ', 'Wash the top and bottom of slide with water and clean the slide bottom well.', 'Counterstain with Methylene Blue for 30 seconds to 1 minute.']
42413
Dip a cotton swab into some gentle isopropyl alcohol (rubbing alcohol) and brush it in the area surrounding each key. You may need to use multiple swabs, depending on the amount of dirt.
311372
['Laboratory for processing and cutting sections. ... ', 'Separate area for staining slides either manually or by machine.', 'Separate area for applying coverslips to slides.', 'Separate room or dedicated area with adequate ventilation/fume extraction for macroscopic examination of formalin-fixed specimens.']
1054885
Wet a cloth or cotton swab with isopropyl alcohol. Rub the cloth over the jeans starting an inch or two outside the spot and moving towards the middle of the spot. The alcohol will loosen the dye on the jeans and the cloth will drag it over to the faded area.
1474676
Pour a small amount of peroxide directly onto an ink stain made from a pen or marker. Allow the peroxide to sit until the stain seems to be dissolving; check it every 10 minutes or so. Absorb the liquid by blotting it with a soft rag. Then wipe it with a damp sponge.
929614
To remove water spots, wash the stain with a gentle detergent and water; use a soft bristled brush to scrub lightly. Rinse with clean water and dry. For slightly more stubborn stains, make a paste of baking soda and water, or talc with a diluted solution of ammonia, bleach or hydrogen peroxide.
1140200
['Place the sample in 1 ml of saline and agitate to mix. Take a drop of this mixture and place it on a slide.', 'Place a drop of saline on a slide and add a small amount of the discharge.']
1295516
['1 Formaldehyde.', '2 Glutaraldehyde.', '3 Ortho-phthalaldehyde.', '4 Hydrogen peroxide.', '5 Peracetic acid.', '6 Hydrogen peroxide/peracetic acid combination.', '7 Sodium hypochlorite.', '8 Iodophors.']
1397597
['Plastic protective capes.', 'Color mixing bowls.', 'Color brushes.', 'Tube squeezers.', 'Bleach applicators.', 'Color removal wipes.', 'Cotton rolls.', 'Cotton balls.']
1117513
['Take a reasonable piece of blue tack and work between your fingers to warm it up.', 'Take your hand soap, and add a little at a time, working it into the blu tack. It will get softer and more malleable as you work. ... ', 'If you want to, you can jazz it up by adding either paint or food colouring at the end.']
1209219
['Select a pure culture plate of one of the organisms to be tested.', 'Aseptically emulsify a colony from the plate in the sterile saline solution. ... ', 'Repeat until the turbidity of the saline solution visually match that of the standard turbidity.', 'Take a sterile swab and dip it into the broth culture of organism.']
102650
Apply stain only at the proper temperature. For most stains, 70 degrees Fahrenheit is the optimum, with the safety range from 50 to 90 degrees Fahrenheit. Stains do vary, so check the label first.
850973
Viscose is extremely absorbent so remove stains using a damp cloth and a mild cleanser. For tough stains, use the stain solution suggested above and gently apply the foam that rises to the top to the stained area. Blot with a clean damp cloth to finish. Try to keep a viscose rug in a cool and dry location.
393941
Place a paper towel under the stain and sponge it with rubbing alcohol. Use an eyedropper to apply alcohol directly onto the stain or, for a larger spot, pour the alcohol into a small dish, immerse the stained area and soak for 15 minutes. The ink should begin to dissolve almost immediately.
935729
Metal stains must be removed with a poultice. (See section on Making & Using a Poultice) Deep-seated, rusty stains are extremely difficult to remove and the stone may be permanently stained. Clean with diluted (1/2 cup in a gallon of water) ammonia OR bleach OR hydrogen peroxide. DO NOT MIX BLEACH AND AMMONIA!
524972
['APPLY a thin layer of IBX over the nail plate.', 'HEAT gently for 4 minutes under the IBX Heat Lamp.', 'BLOT the excess from the surface lightly if needed.', 'CURE with an LED or UV Lamp for the same amount of time your lamp cures your Gel Polish colour coat.']
1098855
['Step 2: Smear Preparation (Review) ... ', 'Cover the smear with carbolfuchsin dye. ... ', 'Dry heat for 2 minutes.', 'Cool and rinse with water. ... ', 'Wash the top and bottom of slide with water and clean the slide bottom well.', 'Counterstain with Methylene Blue for 30 seconds to 1 minute.']