what is tntc in microbiology?
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TNTC. Too numerous to count Lab medicine A popular abbreviation for a 'lawn' of bacteria on a culture plate that may be seen in UTIs–confluent growth is equal to ±105 colonies Medtalk An abbreviation facetiously used for a hypochondriac Pt's list of complaints.
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The MTT assay is a colorimetric assay for assessing cell metabolic activity. NAD(P)H-dependent cellular oxidoreductase enzymes may, under defined conditions, reflect the number of viable cells present. ... With WST-1, which is cell-impermeable, reduction occurs outside the cell via plasma membrane electron transport.
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colonies. ◦ TNTC (Too Numerous To Count): more than. 300 colonies. ◦ TFTC (Too Few To Count): less than 30 colonies.
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MTT and related tetrazolium salts The degree of light absorption is dependent on the degree of formazan concentration accumulated inside the cell and on the cell surface. The greater the formazan concentration, the deeper the purple colour and thus the higher the absorbance.
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ATCC or the American Type Culture Collection is a nonprofit organization which collects, stores, and distributes standard reference microorganisms, cell lines and other materials for research and development.
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The microtubule-organizing center (MTOC) is a structure found in eukaryotic cells from which microtubules emerge. MTOCs have two main functions: the organization of eukaryotic flagella and cilia and the organization of the mitotic and meiotic spindle apparatus, which separate the chromosomes during cell division.
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Drug resistance can be detected using special laboratory tests which test the bacteria for sensitivity to the drugs or detect resistance patterns. These tests can be molecular in type (such as Xpert MTB/RIF) or else culture-based.
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The TST stands for Time, Steam and Temperature. These are three critical parameter which need to be achieved to guarantee the effectiveness of the sterilisation process. These test strips are chemical indicators and gives instantaneous results.
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The MTT assay is used to measure cellular metabolic activity as an indicator of cell viability, proliferation and cytotoxicity. ... The viable cells contain NAD(P)H-dependent oxidoreductase enzymes which reduce the MTT to formazan.
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abbreviation. TNT is defined as an abbreviation for the explosive trinitrotoluene. An example of TNT is the compound used in dynamite.
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Enterobacteriaceae is a family of Gram-negative, facultatively anaerobic, non-spore-forming rods. Characteristics of this family include being motile, catalase positive, and oxidase negative; reduction of nitrate to nitrite; and acid production from glucose fermentation. However, there are also many exceptions.
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More about Travel Bugs and trackables. TFTC. "Thanks For The Cache". An acronym written by geocachers in logbooks or online when logging cache finds. Occasionally written as T4TC.
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Principle. The assay is based on the cleavage of the tetrazolium salt XTT in the presence of an electron-coupling reagent, producing a soluble formazan salt. This conversion only occurs in viable cells. Cells grown in a 96-well tissue culture plate are incubated with the XTT labeling mixture for 2 - 20 hours.
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DNA gyrase and topoisomerase IV are the two type II topoisomerases present in bacteria. ... Gyrase is involved primarily in supporting nascent chain elongation during replication of the chromosome, whereas topoisomerase IV separates the topologically linked daughter chromosomes during the terminal stage of DNA replication.
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Bacteria. The ATCC Bacteriology Collection is a diversified assemblage of prokaryotes, containing more than 18,000 strains in over 750 genera. The Collection holds more than 3,600 type cultures of validly described species, and nearly 500 bacteriophages..
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Basically, applied microbiology is a subset of microbiology. Microbiology refers to the general study of microscopic organisms, while applied microbiology is the study of the way the microbial world interacts with our own and how we can utilize microbes in various processes.
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Taq is a DNA polymerase. The enzyme manufactures a strand of DNA that is complimentary to a single strand of DNA. All bacteria possess DNA polymerase. The reason that the taq polymerase has become so significant to biotechnological processes is because of the resistance of the enzyme to heat.