 is dominant on gentle slopes in the centre of the reserve. Common plant species include southern blue gum (Eucalyptus bicostata), broad-leaved peppermint (Eucalyptus dives), red stringybark (Eucalyptus macrorhyncha), Robertson's peppermint (Eucalyptus robertsonii ssp. robertsonii), common bracken (Pteridium esculentum), silver wattle (Acacia dealbata), shiny cassinia (Cassinia longifolia), Hovea linearis, honeypots (Acrotriche serrulata), Platylobium formosum ssp. formosum, pale wedge pea (Gompholobium huegelii), Monotoca scoparia, twyning glycine (Glycine clandestina), rough bedstraw (Galium gaudichaudii), native geranium (Geranium solanderi var. solanderi), Plantago varia, Brachyscome spathulata, pink fairy orchid (Caladenia carnea var. carnea), kidneyweed (Dichondra repens), cudweed (Euchiton gymnocephalus), nodding greenhood (Pterostylis nutans), prickly starwort (Stellaria pungens), Australian bluebell (Wahlenbergia stricta ssp. stricta), common wheatgrass (Elymus scaber var. scaber), wattle mat-rush (Lomandra filiformis ssp. filiformis) and snow grass (Poa sieberiana var. hirtella and Poa sieberiana var. cyanophylla).

Threatened species
Two species listed on the  are known to exist in the reserve. The Yass daisy is listed as vulnerable, and the crimson spider orchid is listed as endangered. There are thought to be about 100 crimson spider orchid plants in the reserve, which constitute one of four known populations of the species in New South Wales.

Grevillea iaspicula meets the criteria for listing as Critically Endangered in NSW under the . Seven sub-populations of the plant are known to occur in the Wee-Jasper–Burrinjuck area; one of these sub-populations is located in the reserve.

Fauna
3 amphibian species, 9 reptile species, 32 mammal species and 179 bird species have been recorded in the reserve since the late 1970s.

Threatened species
16 animal species known to exist in the reserve are listed under the . All are listed as vulnerable. They are the gang-gang cockatoo (Callocephalon fimbriatum), t