f the strong points of this study is that it should provide pregnant women with accumulated information on preventive knowledge against allergy that can be effective in some cases, and the women can apply a combination of these behaviors before and after pregnancy. However, our trial has a few limitations. First, the trial may not provide clear data on the association between individual preventive measures and risk factors for allergy because the mothers themselves will be responsible for selecting and applying a combination of preventive measures. The second limitation is the study population because our sample is a relatively homogeneous group of pregnant women who are giving birth in a single hospital. Therefore, our findings may not be generalizable to other institutions or communities. However, at least in present-day Japan, a system for pregnant women to receive education is already in place, so we hope that all hospitals will have the opportunity to conduct our educational program.

Currently, most pregnant women in Japan are concerned about the potential development of allergies in their babies. If the results of the study indicate that the educational program has a positive effect on preventing allergies, "prenatal education to prevent allergy in infants" should be added to the existing childbirth educational program provided by medical institutions and local governments or the physician's instructions provided during infant examinations. This may decrease the anxiety of pregnant women and prevent allergy in infants. Our educational program is based on research findings from papers published in international medical journals. Thus, this program, which is the first study to determine the effect of education on allergy prevention, is expected to be effective both within and outside the Japanese population. The results of our program will be publicized to help change the behaviors of mothers and, if the program is effective for preventing allergies in infants, it will be disclosed worldwide as a new prevent