 Moreover, yeast transformants expressing a known efflux pump (PDR5) showed similar trends in propanol transport inhibition compared to 4D. ABC transporters, such as PDR5, are ubiquitous in many different groups of microorganisms ([@bib28]), and some act as efflux pumps for a wide spectrum of substances ([@bib28]). PDR5 has been recently found to be actively produced even under conditions of nutrient limitation, because it is important for the survival in the presence of toxic compounds that can be pumped out of cells ([@bib23]). The relative expression of 4D under nutrient-limiting conditions is unknown, and was beyond the scope of the present study. Further research is warranted to examine the potential for 4D to transport mycotoxins other than deoxynivalenol. We don\'t currently know the level of specificity of 4D for DON, and comparisons with the parent DON-sensitive yeast strain in the screening experiments suggest that there may be some cost to expressing the transporter. Our work extends previous studies that have demonstrated the potential of microbial strategies in DON reduction. The transporter 4D, along with other candidates from our library of microbial DNA fragments, could be used to develop transgenic plants to limit DON accumulations in seeds. For example, resistance of tobacco plants to *Fusarium* was increased by the insertion of *PDR5* ([@bib20]).

CRediT authorship contribution statement {#sec5}
========================================

**Celia Jimenez-Sanchez:** Writing - review & editing. **Nina Wilson:** Writing - review & editing. **Nicole McMaster:** Writing - review & editing. **Dash Gantulga:** Writing - review & editing. **Benjamin G. Freedman:** Writing - review & editing. **Ryan Senger:** Writing - review & editing. **David G. Schmale:** Writing - review & editing.

Declaration of competing interest
=================================

The authors declare no conflict of interest.

Abbreviations {#appsec1}
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GC/MSGas Chromatography Mass SpectrometeryDOP-PCROligonucleotide Pr